Statistical analysis of ocular monochromatic aberrations in Chinese population for adaptive optics ophthalmoscope design

It is necessary to know the distribution of the Chinese eye’s aberrations in clinical environment to guide high-resolution retinal imaging system design for large Chinese population application. We collected the monochromatic wave aberration of 332 healthy eyes and 344 diseased eyes in Chinese population across a 6.0-mm pupil. The aberration statistics of Chinese eyes including healthy eyes and diseased eyes were analyzed, and some differences of aberrations between the Chinese and European race were concluded. On this basis, the requirement for adaptive optics (AO) correction of the Chinese eye’s monochromatic aberrations was analyzed. The result showed that a stroke of 20[Formula: see text][Formula: see text]m and ability to correct aberrations up to the 8th Zernike order were needed for reflective wavefront correctors to achieve near diffraction-limited imaging in both groups for a reference wavelength of 550[Formula: see text]nm and a pupil diameter of 6.0[Formula: see text]mm. To verify the analysis mentioned above, an AO flood-illumination system was established, and high-resolution retinal imaging in vivo was achieved for Chinese eye including both healthy and diseased eyes.

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In vivo high-resolution fluorescence retinal imaging with adaptive optics

Frontiers in Optics ◽

10.1364/fio.2006.fmm2 ◽

2006 ◽

Author(s):

Daniel C. Gray ◽

William Merigan ◽

Bernard P. Gee ◽

Jessica I. Wolfing ◽

Jason Porter ◽

...

Keyword(s):

High Resolution ◽

Adaptive Optics ◽

Retinal Imaging

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High Resolution Retinal Imaging Based on Ocular Aberration Measurement and Compensation

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.246-247.213 ◽

2012 ◽

Vol 246-247 ◽

pp. 213-218

Author(s):

Chun Liang ◽

Jian Xin Shen ◽

Sai Sai Niu

Keyword(s):

High Resolution ◽

Adaptive Optics ◽

Imaging System ◽

Low Cost ◽

Critical Role ◽

Retinal Imaging ◽

Retinal Disease ◽

Retinal Cell ◽

Wavefront Aberration ◽

Diagnostic Modality

Ocular retinal imaging is a major diagnostic modality for retinal disease, and can play a critical role for diagnosing systemic diseases such as diabetes and eye-specific diseases such as macular degeneration and diabetic retinopathy, the leading causes of blindness. In order to get high-resolution retinal imaging and develop the low-cost and compact retinal imaging system, we employ micro adaptive optics, which is consisted of wavefront sensor, wavefront corrector and control system. In this paper, the theory, design and testing of the ocular retinal microscopy is detailed, with an emphasis on the eye wavefront aberration describing, aberration detecting method with Hartmann-Shack wavefront sensing and close-loop aberration compensating by micromachined membrane deformable mirrors(MMDM).The ocular retinal microscopy experimental setup is built, the retinal cell imaging had been snapped. It is showed in this work that the ocular retinal microscopy based on adaptive optics system can enable diffraction-limited imaging of micro-scale features of the retina, through real-time compensation of aberrations introduced by the eye.

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Challenges and possibilities for developing adaptive optics: ultra-high resolution optical coherence tomography for clinical in vivo retinal imaging

10.1117/12.819169 ◽

2008 ◽

Author(s):

Robert J. Zawadzki ◽

Stacey S. Choi ◽

Julia W. Evans ◽

John S. Werner

Keyword(s):

Optical Coherence Tomography ◽

High Resolution ◽

Adaptive Optics ◽

Retinal Imaging ◽

Optical Coherence

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Adaptive-optics optical coherence tomography for high-resolution and high-speed in vivo retinal imaging

10.1117/12.632962 ◽

2005 ◽

Author(s):

Robert J. Zawadzki ◽

Stacey Choi ◽

Sophie Laut ◽

John S. Werner ◽

Steven M. Jones ◽

...

Keyword(s):

Optical Coherence Tomography ◽

High Resolution ◽

Adaptive Optics ◽

High Speed ◽

Retinal Imaging ◽

Optical Coherence

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High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

10.1101/2020.09.12.294421 ◽

2020 ◽

Author(s):

Congping Chen ◽

Zhongya Qin ◽

Sicong He ◽

Shaojun Liu ◽

Shun-Fat Lau ◽

...

Keyword(s):

High Resolution ◽

Adaptive Optics ◽

Pyramidal Neurons ◽

Imaging System ◽

Superficial Layer ◽

Wavefront Sensing ◽

Two Photon Microscopy ◽

Two Photon ◽

Transcranial Imaging

AbstractImaging of the brain in its native state at high resolution poses major challenges to visualization techniques. Two-photon microscopy integrated with the thinned-skull or optical clearing skull technique provides a minimally invasive tool for in vivo imaging of the cortex of mice without activating immune response and inducing brain injury. However, the imaging contrast and resolution are severely compromised by the optical heterogeneity of the skull, limiting the imaging depth to the superficial layer. Here, we develop adaptive optics two-photon microscopy for high-resolution transcranial imaging of layer 5 pyramidal neurons up to 700 μm below pia in living mice. In particular, an optimized configuration of imaging system and new wavefront sensing algorithm are proposed for accurate correction for the aberrations induced by the skull window and brain tissue. We investigated microglia-plaque interaction in living brain of Alzheimer’s disease and demonstrated high-precision laser dendrotomy and single-spine ablation.

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