Viewport Rendering Algorithm with a Curved Surface for a Wide FOV in 360° Images

Omnidirectional visual content has attracted the attention of customers in a variety of applications because it provides viewers with a realistic experience. Among the techniques that are used to construct omnidirectional media, a viewport rendering technique is one of the most important modules. That is because users can perceptually evaluate the quality of the omnidirectional service based on the quality of the viewport picture. To increase the perceptual quality of an omnidirectional service, we propose an efficient algorithm to render the viewport. In this study, we analyzed the distortions in the viewport picture to be the result of sampling. This is because the image data for the unit sphere in the omnidirectional visual system are non-uniformly sampled to provide the viewport picture in the conventional algorithms. We propose an advanced algorithm to construct the viewport picture, in which the viewport plane is constructed using a curved surface, whereas conventional methods use flat square surfaces. The curved surface makes the sampling interval more equally spaced than conventional techniques. The simulation results show that the proposed technique outperforms the conventional algorithms with respect to the objective quality based on the criteria of straightness, conformality, and subjective perceptual quality.

A Novel Approach to Blood Smear Analysis Based on Specimen Topology: Implications for Human and Artificial Intelligence Decision Making

Complete blood count (CBC) analysis is one of the most commonly ordered laboratory tests and is a critical first step in patients' clinical evaluation. However, CBC analyzers are limited in their ability to positively identify several types of white blood cells (WBC), and cells with substantial clinical significance, such as immature granulocytes or blasts, are merely marked as flags. Also, CBC analyzers fall short of recognizing informative red blood cell (RBC) morphology, such as schistocytes, and often provide inaccurate platelets count. Flags and clinically non-sufficient CBC-derived data reflex to generation of blood smear (BS), and BS review comprises a substantial portion of the workload in routine hematology laboratories. For accurate identification and classification of WBC, BS analysis (BSA) requires detailed observation of cells with high-magnification objective (60-100X), which provides a relatively narrow Field of View (FOV). This physical limitation restricts current BSA to either low resolution/wide FOV or to high resolution/narrow FOV data generation (Fig. 1A). Hence, key issues of BSA such as the effects of the smearing process on the distribution of blood components, the effects of cells distribution on their morphology and further classification, as well as many other attributes, are addressed only qualitatively or empirically, leaving the real topology of the BS obscure. The computational imaging microscopy system presented herein uses a low resolution and wide FOV objective, and records a plurality of images under different illumination conditions, of the same sample area (Fig. 1B). An algorithm reconstructs a high resolution and aberration free image of whole specimens, as can be observed in the attached link (https://tinyurl.com/Scopio-Labs-X100-ASH-2020). High resolution images are critical not only for manual BSA, but also for artificial intelligence (AI)-derived BSA, since data quality is of prime importance for deep-learning processes, and to a large extent determine their outcome. Thus, the combination of high resolution/wide FOV turns each BS into a big data analytic field, rendering the measurement of yet undetermined cell characteristics. In order to elucidate the basic topology, 60 normal BS (28 females, 32 males) were subjected to analysis utilizing this novel computational imaging microscopy. For convenience of analysis and comparison with current BSA methodology, BS were segmented into strips according to RBC density (Fig. 1C, D). The average length of smear from females (F) was higher by nearly 28% compared with smear from males (M), and the presence of acute inflammation (A) resulted in a significant 33% increase in overall smear length compared to normal (N) average (Fig. 1E). As expected, RBC density formed a linear gradient (Fig. 1C) along the axis of sample smearing, however, RBC morphology was affected by location within the BS. For example, strips 4-5 contained RBC with the appearance of spherocytes (Fig. 1F; arrows), while in strips with increased RBC density, cells aggregated resembling rouleaux formation (Fig. 1F; arrowheads). Platelets distribution was non-linear, with only a few of them reaching the feathered edge of the smear (Fig. 1G). Since the variance of both RBC/FOV and platelets/FOV concentrations drops starting with strip 4, BS-derived platelets number estimates should not be performed in strips 1-3. On average, a normal BS contains 890+399 WBC in the scanned area (strips 1-8). Similar to RBC, the location of individual WBC throughout the BS may affect their morphology, and hence their classification. WBC in the feathered edge (strips 1-3) are generally more stretched, and often squeezed between RBC, rendering their classification by AI-based tools challenging (Fig. 1H). In strips 4-7, WBC morphology is optimal for a classification task, enabling favorable outcomes for either manual or AI cell analysis (Fig. 1H). These data indicate that BSA can be taken to a sensitivity level of at least 10-3 of WBC analysis, provided that a large portion of the BS is scanned. Our system provides a novel combination of computational imaging microscopy and AI-based classification tools to unravel the complex topology of blood smears, and upgrade the data obtained in BSA. This approach enables the establishment of quantitative rules to scientifically direct the objective analysis of cellular blood components both manually, and by AI-tools. Figure Disclosures Katz: Scopio Labs: Consultancy.