(Invited) Elucidating the Role of Mo during the Passivation of Ni-Cr-Mo Alloys: Real Time Analysis of Elemental Dissolution and Surface Enrichment

2020 ◽  
Vol MA2020-02 (12) ◽  
pp. 1262-1262
Author(s):  
Xuejie Li ◽  
Jeffrey D. Henderson ◽  
David W. Shoesmith ◽  
James Noel ◽  
Kevin Ogle
Keyword(s):  
Real Time ◽  
Surface Enrichment ◽  
Time Analysis ◽  
Real Time Analysis

scholarly journals Correction: Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm

2005 ◽  
Vol 170 (7) ◽  
pp. 1171-1171
Author(s):  
Christopher D. Wood ◽  
Takuya Nishigaki ◽  
Toshiaki Furuta ◽  
Shoji A. Baba ◽  
Alberto Darszon
Keyword(s):  
Real Time ◽  
Sea Urchin ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Sea Urchin Sperm

scholarly journals Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics

2019 ◽  
Author(s):  
Nicole C. Robb ◽  
Aartjan J.W. te Velthuis ◽  
Ervin Fodor ◽  
Achillefs N. Kapanidis
Keyword(s):  
Real Time ◽  
Virus Replication ◽  
Dynamic Equilibrium ◽  
Influenza Viruses ◽  
Time Analysis ◽  
Template Strand ◽  
Real Time Analysis ◽  
Influenza Virus Replication ◽  
Multiple Conformations

ABSTRACTThe viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilised vRNA and cRNA initiation complexes in real-time. Our results show that, while the 3’ terminus of the vRNA promoter exists in dynamic equilibrium between pre-initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics. Two residues in the proximal 3’ region of the cRNA promoter (residues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active site, limiting promoter dynamics. Our results highlight promoter-dependent differences in influenza initiation mechanisms, and advance our understanding of virus replication.

scholarly journals Real-time analysis of single influenza virus replication complexes reveals large promoter-dependent differences in initiation dynamics

2019 ◽  
Vol 47 (12) ◽  
pp. 6466-6477 ◽  
Author(s):  
Nicole C Robb ◽  
Aartjan J W te Velthuis ◽  
Ervin Fodor ◽  
Achillefs N Kapanidis
Keyword(s):  
Real Time ◽  
Virus Replication ◽  
Dynamic Equilibrium ◽  
Influenza Viruses ◽  
Time Analysis ◽  
Template Strand ◽  
Real Time Analysis ◽  
Influenza Virus Replication ◽  
Multiple Conformations

AbstractThe viral RNA (vRNA) genome of influenza viruses is replicated by the RNA-dependent RNA polymerase (RNAP) via a complementary RNA (cRNA) intermediate. The vRNA promoter can adopt multiple conformations when bound by the RNAP. However, the dynamics, determinants, and biological role of these conformations are unknown; further, little is known about cRNA promoter conformations. To probe the RNA conformations adopted during initial replication, we monitored single, surface-immobilized vRNA and cRNA initiation complexes in real-time. Our results show that, while the 3′ terminus of the vRNA promoter exists in dynamic equilibrium between pre-initiation and initiation conformations, the cRNA promoter exhibited very limited dynamics. Two residues in the proximal 3′ region of the cRNA promoter (residues absent in the vRNA promoter) allowed the cRNA template strand to reach further into the active site, limiting promoter dynamics. Our results highlight promoter-dependent differences in influenza initiation mechanisms, and advance our understanding of virus replication.

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

scholarly journals Real-Time Analysis of Animal Feeding Behavior With a Low-Calculation-Power CPU

2020 ◽  
Vol 67 (4) ◽  
pp. 1197-1205 ◽  
Author(s):  
Yuki Totani ◽  
Susumu Kotani ◽  
Kei Odai ◽  
Etsuro Ito ◽  
Manabu Sakakibara
Keyword(s):  
Feeding Behavior ◽  
Real Time ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Animal Feeding

A threshold-based, real-time analysis in early detection of endpoint anomalies using SIEM expertise

2021 ◽  
Vol 2021 (4) ◽  
pp. 7-16
Author(s):  
Sivaraman Eswaran ◽  
Aruna Srinivasan ◽  
Prasad Honnavalli
Keyword(s):  
Early Detection ◽  
Real Time ◽  
Time Analysis ◽  
Real Time Analysis

Chromo-fluorogenic sensors for chemical warfare agents in real-time analysis: journey towards accurate detection and differentiation

2021 ◽  
Vol 57 (28) ◽  
pp. 3430-3444
Author(s):  
Vinod Kumar
Keyword(s):  
Real Time ◽  
Chemical Warfare Agents ◽  
Chemical Warfare ◽  
Chemical Probes ◽  
Time Analysis ◽  
Specific Detection ◽  
Success Stories ◽  
Real Time Analysis ◽  
Warfare Agents ◽  
Unique Chemical

This article describes our journey and success stories in the development of chemical warfare detection, detailing the range of unique chemical probes and methods explored to achieve the specific detection of individual agents in realistic environments.

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