Regulation of cholesteryl ester transfer activity in adipose tissue: comparison between hamster and rat species

1995 ◽  
Vol 269 (1) ◽  
pp. E99-E107 ◽  
Author(s):  
G. X. Shen ◽  
A. Angel
Keyword(s):  
Adipose Tissue ◽  
Cholesteryl Ester ◽  
Cholesteryl Ester Transfer Protein ◽  
Saturated Fat ◽  
Transfer Protein ◽  
Transfer Activity ◽  
Dose Dependent Fashion ◽  
Rat Adipose Tissue

The present study demonstrates cholesteryl ester transfer activity (CETA) in cultured hamster and rat adipose tissue. Cultured hamster and rat adipose tissue fragments released CETA into the conditioned medium, and this was associated with a reciprocal decrease in adipose tissue CETA. Regional variations in adipose CETA were observed. The levels of CETA released from cultured hamster and rat adipocytes were higher than those from adipose tissue fragments. In hamsters but not in rats, the secretion of CETA from cultured adipose tissue was increased by insulin and inhibited by EDTA in a dose-dependent fashion. Monoclonal antibodies against human cholesteryl ester transfer protein inhibited the CETA secreted from hamster adipose tissue but not that from rat adipose tissue. Fasting for 24 h and a high-cholesterol saturated fat-rich diet increased adipose CETA in hamsters and rats, and this was associated with an elevation of plasma CETA only in hamsters. This supports the view that, in hamsters, adipose CETA has in situ and intravascular functions, whereas in rats the role of adipose CETA is restricted to tissue-specific functions. Hamster cholesteryl ester transfer protein may differ from rat adipose-associated CETA in the structure of the active site and the regulatory mechanism for its secretion.

Novel role of cholesteryl ester transfer protein (CETP): attenuation of adiposity by enhancing lipolysis and brown adipose tissue activity

Metabolism ◽  
2021 ◽  
Vol 114 ◽  
pp. 154429
Author(s):  
Helena F. Raposo ◽  
Patricia Forsythe ◽  
Bruno Chausse ◽  
Júlia Z. Castelli ◽  
Pedro M. Moraes-Vieira ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Cholesteryl Ester ◽  
Brown Adipose Tissue ◽  
Cholesteryl Ester Transfer Protein ◽  
Transfer Protein ◽  
Brown Adipose ◽  
Brown Adipose Tissue Activity

scholarly journals Genotyping of 2 Cholesteryl Ester Transfer Protein Gene Variants Among Coronary Artery Disease Patients

2020 ◽  
Vol 5 (3) ◽  
pp. 96-100
Author(s):  
Saeid Morovvati ◽  
Nima Kazemi Koohbanani ◽  
Iman Salahshouri Far ◽  
Fatemeh Karami
Keyword(s):  
Coronary Artery ◽  
Cholesteryl Ester ◽  
Cholesteryl Ester Transfer Protein ◽  
Protein Function ◽  
Cholesterol Metabolism ◽  
Density Lipoprotein ◽  
Transfer Protein ◽  
Polymerase Chain ◽  
Artery Disease

Introduction: Coronary artery diseases (CAD) are still among the top causes of death in most populations. The polymorphisms of the cholesteryl ester transfer protein (CETP) gene can influence the risk of CAD through modulating cholesterol metabolism. In this regard, the current study aimed to determine the role of the 2 important CETP gene polymorphisms in CAD patients. Methods: To this end, DNA was extracted from the whole blood of 100 CAD patients and 100 healthy controls and then subjected to polymerase chain reaction-restriction fragment length polymorphism for the genotyping of rs5882 and rs708272 polymorphisms.Results: Based on the results, no meaningful association was found between rs5882 and rs708272 polymorphisms, neither separately nor in combination, and the risk of CAD. However, the risk of CAD significantly increased in male rs5882 polymorphism carriers (P = 0.01). Finally, no significant association was demonstrated between serum high-density lipoprotein levels and the genotypes or alleles of neither rs5882 nor rs708272 polymorphism. Conclusion: Despite the finding regarding the lack of an association between CAD and the studied polymorphisms of the CETP gene, the importance of those variants in CETP protein function and CAD pathogenesis warrants further investigation on larger populations.

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