Optimal cutting temperature medium embedding and cryostat sectioning are valid for cardiac myofilament function assessment
2020 ◽
Vol 319
(1)
◽
pp. H235-H241
Keyword(s):
Myocardial tissue in optimal cutting temperature (OCT) fixation and cryostat sectioning was tested as a means of storing and preparing tissue for myofilament function analysis in relation to conventional liquid nitrogen freezing and dissection. Actomyosin interaction, Ca2+ force activation, and passive compliance were tested. The study concluded that OCT storage and cryostat sectioning do not interfere with the actomyosin cross-bridge dynamics or Ca2+ activation but that absolute tension values suffer and may not be investigated by this method.
1995 ◽
Vol 268
(6)
◽
pp. C1348-C1353
◽
Keyword(s):
GW28-e1113 Pim-1 Kinase Phosphorylates Cardiac Troponin I and Regulates Cardiac Myofilament Function
2017 ◽
Vol 70
(16)
◽
pp. C44
Keyword(s):
Keyword(s):
2006 ◽
Vol 40
(6)
◽
pp. 893-894
2013 ◽
Vol 21
(12)
◽
pp. 567-571
◽
2021 ◽
Vol 63
(1/2)
◽
pp. 1
Keyword(s):