BIOASSAY OF STEROID HORMONES USING AQUEOUS SODIUM LAURYL SULFATE SOLUTION AS THE DISPERSING AGENT

1947 ◽  
Vol 150 (3) ◽  
pp. 444-450 ◽  
Author(s):  
Fritz Bischoff ◽  
Harry R. Pilhorn
1970 ◽  
Vol 74 (11) ◽  
pp. 2324-2326
Author(s):  
Kenneth Beard ◽  
Michael Rios ◽  
Douglas Currell ◽  
Richard Reis

1994 ◽  
Vol 77 (5) ◽  
pp. 1153-1156
Author(s):  
Marvin J Nakashima

Abstract A collaborative study was conducted on an alternative sieving method for the extraction of light filth from cheeses. The alternative method was developed that is applicable to broad variety of cheeses. A 225 g test portion is dispersed in a solution of 5.7% HCI, Igepal CO-730, and Igepal DM-710. Digested cheese is wet-sieved on a No. 230 sieve. The residue is treated with Tergitol Anionic 4, transferred to 1% sodium lauryl sulfate solution, heated, and maintained at 65°-75°C for 10 min. The residue is washed with these 2 surfactants a maximum of 4 times until it is reduced to an amount that is filterable. The residue is filtered and the filter papers are examined microscopically at a magnification of ca 30×. Average recoveries by 9 collaborators for 3 spike levels of rat hairs (5,10, and 15) were 80, 68, and 81%, respectively; for insect fragments (5,15, and 30) recoveries were 97, 90, and 92%, respectively. The alternative sieving method for extraction of light filth from cheeses has been adopted first action by AOAC INTERNATIONAL.


Cosmetics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 6
Author(s):  
Sabrina Leoty-Okombi ◽  
Florence Gillaizeau ◽  
Sébastien Leuillet ◽  
Benoit Douillard ◽  
Sophie Le Fresne-Languille ◽  
...  

In this study, we assessed the change in skin microbiota composition, relative abundance, and diversity with skin physiology disruption induced by SLS patch. Healthy women declaring to have a reactive skin were submitted to a 0.5% aqueous sodium lauryl sulfate solution application under occlusive patch condition for 24 h. Skin properties were characterized by tewametry, corneometry, and colorimetry and bacterial diversity was assessed by 16S rRNA sequencing. Analysis before and one day after SLS patch removal revealed an increase of skin redness and a decrease of stratum corneum hydration and skin barrier function. The relative abundance of taxa containing potential pathogens increase (Firmicutes: Staphylococcaceae; Proteobacteria: Enterobacteriaceae, Pantoea) while some of the most occurring Actinobacteria with valuable skin protection and repair capacities decreased (Micrococcus, Kocuria, and Corynebacterium). We observed an impaired skin barrier function and dehydration induced by SLS patch disturb the subtle balance of skin microbiota towards skin bacterial community dysbiosis. This study provides new insights on the skin bacterial composition and skin physiology simultaneously impaired by a SLS patch.


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