Increased heat-escape/cold-seeking behavior following hypertonic saline injection in rats

2001 ◽  
Vol 280 (4) ◽  
pp. R1031-R1036 ◽  
Author(s):  
Kei Nagashima ◽  
Sadamu Nakai ◽  
Masahiro Konishi ◽  
Liu Su ◽  
Kazuyuki Kanosue
Keyword(s):  
Osmotic Stress ◽  
Hypertonic Saline ◽  
Core Temperature ◽  
Defense Mechanisms ◽  
Specific Area ◽  
Saline Injection ◽  
Lower Baseline ◽  
Seeking Behavior ◽  
Hypertonic Saline Injection

We examined the effect of hypertonic saline injection on heat-escape/cold-seeking behavior in desalivated rats. Rats were exposed to 40°C heat after normal (154 mM NaCl, control) or hypertonic saline (2,500 mM NaCl) injection (1 ml/100 g body wt). The rats received a 0°C air for 30 s when they entered a specific area in an experimental box. Core temperature (Tc) surpassed 40°C in both conditions when 0°C air was not available. Hypertonic saline injection produced a lower baseline Tcthan control [36.9 ± 0.2 and 37.9 ± 0.2°C (means ± SE), P < 0.05] and a greater number of 0°C air rewards during the 2-h heat with lower Tc at the end (48 ± 1 and 34 ± 2, 37.6 ± 0.1, and 37.3 ± 0.1°C in the control and hypertonic saline injection trial, respectively, P < 0.05, n = 6). However, Tc was similar (37.7 ± 0.2 and 37.6 ± 0.4°C in the control and hypertonic saline injection trial, n = 5) when 0°C air was automatically and intermittently (35 times) given during the heat. Rats augment heat-defense mechanisms in response to osmotic stress by lowering the baseline Tc and increasing heat-escape/cold-seeking behavior.

Use of hypertonic saline injection in trauma

2010 ◽  
Vol 67 (22) ◽  
pp. 1920-1928 ◽  
Author(s):  
Asad E. Patanwala ◽  
Albert Amini ◽  
Brian L. Erstad
Keyword(s):  
Hypertonic Saline ◽  
Saline Injection ◽  
Hypertonic Saline Injection

Long-term Potentiation of Orofacial Sensorimotor Processing by Noxious Input from the Semispinal Neck Muscle in Mice

Cephalalgia ◽  
2005 ◽  
Vol 25 (2) ◽  
pp. 109-116 ◽  
Author(s):  
A Makowska ◽  
C Panfil ◽  
J Ellrich
Keyword(s):  
Hypertonic Saline ◽  
Long Term Potentiation ◽  
Tension Type Headache ◽  
Neck Muscle ◽  
Saline Injection ◽  
Sensorimotor Processing ◽  
Term Potentiation ◽  
Type Headache ◽  
Hypertonic Saline Injection

Tension-type headache is the most common type of primary headaches but no conclusive concept of pathophysiology exists. This may be due to a lack of an appropriate animal model. This study addressed the hypothesis that noxious neck muscle input induces central sensitization of orofacial sensorimotor processing. The effect of hypertonic saline injection into the semispinal neck muscle on the jaw-opening reflex (JOR) was investigated in anaesthetized mice ( n = 11). Hypertonic saline injection into the neck muscle facilitated the JOR for at least one hour: integral (+94.5%) and duration (+18.7%) increased, latency decreased (-7.5%). The reflex threshold decreased to 61% after injection. Isotonic saline injection into the neck muscle ( n = 11) or hypertonic saline injection into a hindpaw muscle ( n = 10) did neither change the reflex integral nor the threshold. Long-term potentiation of the JOR by noxious neck muscle input may be an appropriate model to investigate tension-type headache pathophysiology.

scholarly journals The Efficacy of Ozone Prolotherapy Compared to Intra-Articular Hypertonic Saline Injection in Reducing Pain and Improving the Function of Patients with Knee Osteoarthritis: A Randomized Clinical Trial

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Hamid reza Farpour ◽  
Alireza Ashraf ◽  
Seyed Saeed Hosseini
Keyword(s):  
Clinical Trial ◽  
Knee Osteoarthritis ◽  
Hypertonic Saline ◽  
Womac Pain ◽  
Saline Injection ◽  
Study Results ◽  
Significant Difference ◽  
The Mean ◽  
Hypertonic Saline Injection ◽  
And Function

Background. Knee osteoarthritis is a common disease that is associated with chronic pain and disability in patients. Prolotherapy is a complementary therapeutic approach for improving pain and function in patients with osteoarthritis. We aimed to compare the effect of hypertonic saline with ozone plus hypertonic saline in improving the symptoms of osteoarthritis in the patients. Materials and Method. In this clinical trial, thirty-four adults with painful primary knee osteoarthritis for at least three months were randomized to two groups: ozone plus hypertonic saline 5% and hypertonic saline 5% alone. Prolotherapy and thrice follow-up with two-week intervals were done. The outcome measures included Oxford Knee Scale (OKS), Western Ontario McMaster University Osteoarthritis Index (WOMAC), and Visual Analog Scale (VAS), which were obtained from the patients before the injection and after the 2nd and 4th weeks after the start of the study. Results. The mean age of the participants was 60.12 ± 7.54 years. There were no statistically significant differences between demographic characteristics before the injection between the two groups ( p  > 0.05). The results showed that VAS and OKS values decreased over time ( p  < 0.001) in each group, but there was no significant difference in the reduction of those between the two treatment groups ( p  = 0.734 and p  = 0.734, respectively). Both interventions improved the mean values of WOMAC pain, WOMAC stiffness, WOMAC act, and WOMAC total. However, there was no significant difference in WOMAC pain reduction rate ( p  = 0.465), WOMAC stiffness rate ( p  = 0.656), WOMAC act rate ( p  = 0.376), and WOMAC total rate between the two methods ( p  = 0.528). Conclusion. The results showed that intra-articular prolozone therapy and hypertonic saline injection can lead to improvement of pain and function in patients with knee osteoarthritis at the same status without any significant difference.

Sign in / Sign up

Export Citation Format

Share Document