A role for NPY overexpression in the dorsomedial hypothalamus in hyperphagia and obesity of OLETF rats

2001 ◽  
Vol 281 (1) ◽  
pp. R254-R260 ◽  
Author(s):  
Sheng Bi ◽  
Ellen E. Ladenheim ◽  
Gary J. Schwartz ◽  
Timothy H. Moran
Keyword(s):  
Mrna Expression ◽  
Potential Role ◽  
Arcuate Nucleus ◽  
Leptin Receptor ◽  
Dorsomedial Hypothalamus ◽  
Oletf Rats ◽  
Receptor Mrna ◽  
Ad Libitum ◽  
Long Evans

Otsuka Long-Evans Tokushima Fatty (OLETF) rats lacking CCK-A receptors are hyperphagic, obese, and diabetic. We have previously demonstrated that these rats have a peripheral satiety deficit resulting in increased meal size. To examine the potential role of hypothalamic pathways in the hyperphagia and obesity of OLETF rats, we compared patterns of hypothalamic neuropeptide Y (NPY), proopiomelanocortin (POMC), and leptin receptor mRNA expression in ad libitum-fed Long-Evans Tokushima (LETO) and OLETF rats and food-restricted OLETF rats that were pair-fed to the intake of LETO controls. Pair feeding OLETF rats prevented their increased body weight and elevated levels of plasma insulin and leptin and normalized their elevated POMC and decreased NPY mRNA expression in the arcuate nucleus. In contrast, NPY expression was upregulated in the dorsomedial hypothalamus (DMH) in pair-fed OLETF rats. A similar DMH NPY overexpression was evident in 5-wk-old preobese OLETF rats. These findings suggest a role for DMH NPY upregulation in the etiology of OLETF hyperphagia and obesity.

The Potential Role of Neuropeptide Y Expression in the Dorsomedial Hypothalamus in Thermoregulation during Lactation and Diet-Induced Obesity

2011 ◽  
pp. P2-431-P2-431
Author(s):  
Shin J Draper ◽  
Alberto Loche ◽  
Chris J Madden ◽  
Susan Smith ◽  
Shaun F Morrison ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Potential Role ◽  
Dorsomedial Hypothalamus ◽  
Diet Induced Obesity

Role of Quercetin on PCBs (Aroclor-1254) Induced Impairment of Dopaminergic Receptor mRNA Expression in Cerebral Cortex of Adult Male Rats

2011 ◽  
Vol 36 (8) ◽  
pp. 1344-1352 ◽  
Author(s):  
Rasiah Pratheepa Kumari ◽  
Kandaswamy Selvakumar ◽  
Senthamilselvan Bavithra ◽  
Rafiq Zumaana ◽  
Gunasekaran Krishnamoorthy ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Mrna Expression ◽  
Adult Male ◽  
Male Rats ◽  
Aroclor 1254 ◽  
Dopaminergic Receptor ◽  
Receptor Mrna

scholarly journals Estrogenic-dependent glutamatergic neurotransmission from kisspeptin neurons governs feeding circuits in females

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Jian Qiu ◽  
Heidi M Rivera ◽  
Martha A Bosch ◽  
Stephanie L Padilla ◽  
Todd L Stincic ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Arcuate Nucleus ◽  
Neuronal Excitability ◽  
Glutamate Release ◽  
Metabotropic Receptors ◽  
Hypothalamic Arcuate Nucleus ◽  
17Β Estradiol ◽  
Arcuate Neurons ◽  
Feeding Circuits

The neuropeptides tachykinin2 (Tac2) and kisspeptin (Kiss1) in hypothalamic arcuate nucleus Kiss1 (Kiss1ARH) neurons are essential for pulsatile release of GnRH and reproduction. Since 17β-estradiol (E2) decreases Kiss1 and Tac2 mRNA expression in Kiss1ARH neurons, the role of Kiss1ARH neurons during E2-driven anorexigenic states and their coordination of POMC and NPY/AgRP feeding circuits have been largely ignored. Presently, we show that E2 augmented the excitability of Kiss1ARH neurons by amplifying Cacna1g, Hcn1 and Hcn2 mRNA expression and T-type calcium and h-currents. E2 increased Slc17a6 mRNA expression and glutamatergic synaptic input to arcuate neurons, which excited POMC and inhibited NPY/AgRP neurons via metabotropic receptors. Deleting Slc17a6 in Kiss1 neurons eliminated glutamate release and led to conditioned place preference for sucrose in E2-treated KO female mice. Therefore, the E2-driven increase in Kiss1 neuronal excitability and glutamate neurotransmission may play a key role in governing the motivational drive for palatable food in females.

scholarly journals Arcuate nucleus kisspeptin response to increased nutrition in rams

2019 ◽  
Vol 31 (11) ◽  
pp. 1682 ◽  
Author(s):  
S. E. Rietema ◽  
P. A. R. Hawken ◽  
C. J. Scott ◽  
M. N. Lehman ◽  
G. B. Martin ◽  
...  
Keyword(s):  
Arcuate Nucleus ◽  
Preoptic Area ◽  
Pulse Generator ◽  
Pulse Frequency ◽  
Nutritional Supplementation ◽  
Environmental Cues ◽  
Dorsomedial Hypothalamus ◽  
Gonadotrophin Releasing Hormone ◽  
Kndy Neurons

Rams respond to acute nutritional supplementation by increasing the frequency of gonadotrophin-releasing hormone (GnRH) pulses. Kisspeptin neurons may mediate the effect of environmental cues on GnRH secretion, so we tested whether the ram response to nutrition involves activation of kisspeptin neurons in the arcuate nucleus (ARC), namely kisspeptin, neurokin B, dynorphin (KNDy) neurons. Rams were given extra lupin grain with their normal ration. Blood was sampled before feeding, and continued until animals were killed for collection of brain tissue at 2 or 11h after supplementation. In supplemented rams, LH pulse frequency increased after feeding, whereas control animals showed no change. Within the caudal ARC, there were more kisspeptin neurons in supplemented rams than in controls and a higher proportion of kisspeptin cells coexpressed Fos, regardless of the time the rams were killed. There were more Fos cells in the mid-ARC and mid-dorsomedial hypothalamus of the supplemented compared with control rams. No effect of nutrition was found on kisspeptin expression in the rostral or mid-ARC, or on GnRH expression in the preoptic area. Kisspeptin neurons in the caudal ARC appear to mediate the increase in GnRH and LH production due to acute nutritional supplementation, supporting the hypothesised role of the KNDy neurons as the pulse generator for GnRH.

Role of 5-ht1b receptors in entrainment disorder of otsuka long evans tokushima fatty (oletf) rats

Neuroscience ◽  
2004 ◽  
Vol 123 (1) ◽  
pp. 201-205 ◽  
Author(s):  
T Shimazoe ◽  
S Nakamura ◽  
K Kobayashi ◽  
S Watanabe ◽  
K Miyasaka ◽  
...  
Keyword(s):  
Oletf Rats ◽  
Long Evans
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