Analysis of continence reflexes by dynamic urethral pressure recordings in a rat stress urinary incontinence model induced by multiple simulated birth traumas

The present study evaluated real-time changes in urethral pressure during the storage phase using a rat model with stress urinary incontinence (SUI) induced by simulated multiple birth traumas and investigated the relationship between urethral continence function and dynamic parameters associated with the changes in urethral pressure. Sprague-Dawley rats were divided into the following two groups: the sham group, which underwent three catheterizations of the vagina without distension at 2-wk intervals, and the vaginal distension (VD) group, which underwent three VDs at 2-wk intervals. After transection of the T8–T9 spinal cord, simultaneous bladder and urethral pressure recordings were performed during intravesical pressure elevation. Urodynamic parameters such as leak point pressure (LPP), urethral baseline pressure (UBP), maximum urethral pressure (MUP), the MUP-UBP differential (dUP) during intravesical pressure elevation, the bladder pressure when urethral contraction begins (Puc), and the bladder pressure at bladder neck opening (Pno) were then measured and compared. Compared with the sham group, LPP, UBP, dUP, MUP, Puc, and Pno were significantly decreased in the VD group. Pressure differences between LPP and Pno and between LPP and UBP (LPP-UBP) were also significantly different in the two groups. However, difference values of LPP and MUP or Pno and UBP were not altered after VD. Our new methods of simultaneous recordings of dynamic changes in bladder and urethral pressures are useful to fully evaluate the functional alterations in urethral continence function in the SUI model induced by multiple VDs. Moreover, LPP-UBP values, which correspond to the difference between Valsalva LPP and maximum urethral closure pressure in clinical urodynamics, would be useful to evaluate the impaired urethral continence function after simulated birth traumas in animal models.

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Daily bilateral pudendal nerve electrical stimulation improves recovery from stress urinary incontinence

Interface Focus ◽

10.1098/rsfs.2019.0020 ◽

2019 ◽

Vol 9 (4) ◽

pp. 20190020 ◽

Cited By ~ 2

Author(s):

Kangli Deng ◽

Brian M. Balog ◽

Dan Li Lin ◽

Brett Hanzlicek ◽

Qi-Xiang Song ◽

...

Keyword(s):

Urinary Incontinence ◽

Electrical Stimulation ◽

Stress Urinary Incontinence ◽

Pudendal Nerve ◽

Sprague Dawley ◽

External Urethral Sphincter ◽

Bdnf Expression ◽

Nerve Crush ◽

Sprague Dawley Rats ◽

Point Pressure

Stress urinary incontinence (SUI) in women is strongly associated with childbirth which injures the pudendal nerve (PN) and the external urethral sphincter (EUS) during delivery. Electrical stimulation (ES) can increase brain-derived neurotrophic factor (BDNF) expression in injured neurons, activate Schwann cells and promote neuroregeneration after nerve injury. The aim of this study was to determine if more frequent ES would increase recovery from SUI in a rat model. Forty female Sprague–Dawley rats underwent either sham injury or pudendal nerve crush (PNC) and vaginal distention (VD) to establish SUI. Immediately after injury, electrodes were implanted at the pudendal nerve bilaterally. Each injured animal underwent sham ES, twice per week ES (2/week), or daily ES of 1 h duration for two weeks. Urethral and nerve function were assessed with leak point pressure (LPP), EUS electromyography and pudendal nerve sensory branch potential (PNSBP) recordings two weeks after injury. LPP was significantly increased after daily ES compared to 2/week ES. EUS neuromuscular junction innervation was decreased after injury with sham ES, but improved after 2/week or daily ES. This study demonstrates that daily bilateral ES to the pudendal nerve can accelerate recovery from SUI. Daily ES improved urethral function more than 2/week ES.

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CLINICAL SCORE PREDICTIVE OF THE ABDOMINAL LEAK POINT PRESSURE (ALPP) <60 CM H2O IN WOMEN WITH STRESS URINARY INCONTINENCE.

10.26226/morressier.59b63e42d462b802923897e3 ◽

2017 ◽

Author(s):

Leandro Arribillaga

Keyword(s):

Urinary Incontinence ◽

Stress Urinary Incontinence ◽

Clinical Score ◽

Leak Point Pressure ◽

Point Pressure

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Faculty of 1000 evaluation for A Randomized, Controlled Clinical Trial of a Novel Intravesical Pressure-Attenuation Device for the Treatment of Stress Urinary Incontinence.

F1000 - Post-publication peer review of the biomedical literature ◽

10.3410/f.718022676.793480103 ◽

2013 ◽

Author(s):

Altaf Mangera

Keyword(s):

Clinical Trial ◽

Urinary Incontinence ◽

Stress Urinary Incontinence ◽

Controlled Clinical Trial ◽

Randomized Controlled Clinical Trial ◽

Intravesical Pressure ◽

Randomized Controlled

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Transurethral versus suprapubic catheterization to test urethral function in rats

Scientific Reports ◽

10.1038/s41598-021-93772-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Kristine Janssen ◽

Kangli Deng ◽

Steve J. A. Majerus ◽

Dan Li Lin ◽

Brett Hanzlicek ◽

...

Keyword(s):

Outlet Obstruction ◽

Bladder Pressure ◽

Sprague Dawley ◽

External Urethral Sphincter ◽

Emg Amplitude ◽

Suprapubic Catheterization ◽

Sphincter Electromyography ◽

Significant Difference ◽

Point Pressure ◽

Urethral Function

AbstractTransurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.

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