Role of 5-HT1A receptors in control of lower urinary tract function in anesthetized rats

The role of 5-hydroxytryptamine (5-HT) 1A (5-HT1A) receptors in lower urinary tract function was examined in urethane-anesthetized female Sprague-Dawley rats. Bladder pressure and the external urethral sphincter electromyogram (EUS EMG) activity were recorded during continuous-infusion transvesical cystometrograms (TV-CMGs) to allow voiding and during transurethral-CMGs (TU-CMGs) which prevented voiding and allowed recording of isovolumetric bladder contractions. 8-Hydroxy-2-(di- n-propylamino)-tetralin (8-OH-DPAT), a 5-HT1A receptor agonist, decreased volume threshold (VT) for initiating voiding and increased contraction amplitude (CA) during TU-CMGs but decreased CA during TV-CMGs. 8-OH-DPAT prolonged EUS bursting as well as the intrabursting silent periods (SP) during voiding. N-[2-[4-(2-methoxyphenyl)-1- piperazinyl]ethyl]- N-(2-pyridinyl)cyclohexanecarboxamine trihydrochloride (WAY-100635), a 5-HT1A antagonist, increased VT, increased residual volume, markedly decreased voiding efficiency, decreased the amplitude of micturition contractions recorded under isovolumetric conditions, and decreased the SP of EUS bursting. These results indicate that activation of 5-HT1A receptors by endogenous 5-HT lowers the threshold for initiating reflex voiding and promotes voiding function by enhancing the duration of EUS relaxation, which should reduce urethral outlet resistance.

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Role of spinal metabotropic glutamate receptors in regulation of lower urinary tract function in the decerebrate unanesthetized rat

Neuroscience Letters ◽

10.1016/j.neulet.2007.04.002 ◽

2007 ◽

Vol 420 (1) ◽

pp. 18-22 ◽

Cited By ~ 14

Author(s):

Mitsuharu Yoshiyama ◽

William C. de Groat

Keyword(s):

Urinary Tract ◽

Glutamate Receptors ◽

Lower Urinary Tract ◽

Metabotropic Glutamate Receptors ◽

Metabotropic Glutamate ◽

Lower Urinary Tract Function ◽

Tract Function ◽

Lower Urinary

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Role of pelvic floor in lower urinary tract function

Autonomic Neuroscience ◽

10.1016/j.autneu.2015.06.003 ◽

2016 ◽

Vol 200 ◽

pp. 43-48 ◽

Cited By ~ 6

Author(s):

Christopher J. Chermansky ◽

Pamela A. Moalli

Keyword(s):

Urinary Tract ◽

Pelvic Floor ◽

Lower Urinary Tract ◽

Lower Urinary Tract Function ◽

Tract Function ◽

Lower Urinary

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Role of Nitric Oxide in Fetal Lower Urinary Tract Function

The Journal of Urology ◽

10.1016/s0022-5347(17)32784-2 ◽

1994 ◽

Vol 152 (2 Part 1) ◽

pp. 510-514 ◽

Cited By ~ 41

Author(s):

Robert A. Mevorach ◽

Guy A. Bogaert ◽

Barry A. Kogan

Keyword(s):

Nitric Oxide ◽

Urinary Tract ◽

Lower Urinary Tract ◽

Lower Urinary Tract Function ◽

Tract Function ◽

Lower Urinary

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The Role of the Periaqueductal Gray Matter in Lower Urinary Tract Function

Molecular Neurobiology ◽

10.1007/s12035-018-1131-8 ◽

2018 ◽

Vol 56 (2) ◽

pp. 920-934 ◽

Cited By ~ 6

Author(s):

Aryo Zare ◽

Ali Jahanshahi ◽

Mohammad-Sajjad Rahnama’i ◽

Sandra Schipper ◽

Gommert A. van Koeveringe

Keyword(s):

Urinary Tract ◽

Gray Matter ◽

Lower Urinary Tract ◽

Periaqueductal Gray ◽

Periaqueductal Gray Matter ◽

Lower Urinary Tract Function ◽

Tract Function ◽

Lower Urinary

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Effects of urethane on reflex activity of lower urinary tract in decerebrate unanesthetized rats

AJP Renal Physiology ◽

10.1152/ajprenal.00574.2012 ◽

2013 ◽

Vol 304 (4) ◽

pp. F390-F396 ◽

Cited By ~ 19

Author(s):

Mitsuharu Yoshiyama ◽

James R. Roppolo ◽

Masayuki Takeda ◽

William C. de Groat

Keyword(s):

Urinary Tract ◽

Lower Urinary Tract ◽

Nmda Antagonist ◽

Suppressive Effect ◽

External Urethral Sphincter ◽

Mk 801 ◽

Pressure Threshold ◽

Lower Urinary Tract Function ◽

Tract Function ◽

Lower Urinary

Effects of urethane on lower urinary tract function were examined in decerebrate unanesthetized rats. During single slow infusion (0.04 ml/min) cystometrograms (CMGs), urethane (0.3 g/kg) increased micturition pressure threshold (PT) by 73%, postvoid residual volume (RV) by 425%, and decreased voiding efficiency (VE) by 57%, but did not change maximal voiding pressure (MVP), closing peak pressure (CPP), bladder compliance, bladder contraction duration (BCD), or volume threshold (VT) for inducing micturition. Lower doses (0.01–0.1 g/kg) did not alter any parameter. During continuous fast infusion (0.21 ml/min) CMGs, urethane at doses of 0.6–1.2 g/kg (iv) markedly decreased CPP by 69–85%, whereas only the largest dose (1.2 g/kg iv) decreased MVP and external urethral sphincter electromyogram activity by 42 and by 80%, respectively. Doses of 0.001–0.6 g/kg did not alter the intercontraction interval and BCD. Taken together, these results suggest that urethral activity, which is essential for efficient voiding, is more sensitive to the suppressive effect of urethane than afferent or efferent mechanisms controlling the bladder. The threshold dose of MK-801 (0.3 mg/kg), an NMDA antagonist, required to decrease MVP and increase VT in urethane (1.2 g/kg)-anesthetized rats, only increased VT in rats treated with a subanesthetic dose of urethane (0.3 g/kg), suggesting a higher sensitivity of the afferent vs. efferent limb of the micturition reflex pathway to urethane-MK-801 interactions. Because effects of urethane persisted after removal of the forebrain, they must be mediated by actions on the brain stem, spinal cord, or peripheral nervous system.

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