Exercise modulates antioxidant enzyme gene expression in rat myocardium and liver

2000 ◽  
Vol 88 (5) ◽  
pp. 1791-1796 ◽  
Author(s):  
D. O. Wilson ◽  
P. Johnson
Keyword(s):  
Glutathione Peroxidase ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Antioxidant Enzyme Activities ◽  
Mrna Levels ◽  
Rnase Protection ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto

Our previous studies have shown that exercise caused changes in the tissue activities of the antioxidant enzymes glutathione peroxidase, superoxide dismutase, and catalase in spontaneously hypertensive (SH) and Wistar-Kyoto (WKY) rats. To determine whether the changes observed were due to changes in mRNA levels of the enzymes, levels of tissue mRNA were determined by quantitative RNase protection assay. Comparisons of tissue enzyme activities and mRNA levels in sedentary and exercised animals showed that, in some cases (e.g., glutathione peroxidase in SH and WKY myocardium), parallel changes in enzyme activity and mRNA levels occurred, whereas in other cases (e.g., catalase in SH and WKY liver), nonparallel changes were found. Exercise of hypertensive rats altered antioxidant enzyme mRNA levels to those seen in normotensive animals in some, but not all, cases. The results suggest that transcriptional control over changes in exercise-related antioxidant enzyme activities is operative in some cases, although in other cases posttranscriptional regulatory mechanisms may exist.

scholarly journals Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression.

1988 ◽  
Vol 8 (11) ◽  
pp. 4851-4857 ◽  
Author(s):  
P Bornstein ◽  
J McKay ◽  
D J Liska ◽  
S Apone ◽  
S Devarayalu
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Human Growth ◽  
Collagen Gene ◽  
Mrna Levels ◽  
3T3 Cells ◽  
Rnase Protection ◽  
First Intron

The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblasts and NIH 3T3 cells. In chick tendon fibroblasts, but not in 3T3 cells, inversion of intronic sequences containing a previously studied 274-base-pair segment, A274, resulted in markedly reduced human growth hormone mRNA levels as determined by an RNase protection assay. This inhibitory effect was largely alleviated when deletions were introduced in the collagen promoter of plasmids containing negatively oriented intronic sequences. Evidence for interaction of the promoter with the intronic segment, A274, was obtained by gel mobility shift assays. We suggest that promoter-intron interactions, mediated by DNA-binding proteins, regulate collagen gene transcription. Inversion of intronic segments containing critical interactive elements might then lead to an altered geometry and reduced activity of a transcriptional complex in those cells with sufficiently high levels of appropriate transcription factors. We further suggest that the deleted promoter segment plays a key role in directing DNA interactions involved in transcriptional control.

Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats

2007 ◽  
Vol 128 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Rafael H. Lambertucci ◽  
Adriana Cristina Levada-Pires ◽  
Luciana V. Rossoni ◽  
Rui Curi ◽  
Tania C. Pithon-Curi
Keyword(s):  
Exercise Training ◽  
Aerobic Exercise ◽  
Antioxidant Enzyme ◽  
Soleus Muscle ◽  
Enzyme Activities ◽  
Aerobic Exercise Training ◽  
Antioxidant Enzyme Activities ◽  
Mrna Levels ◽  
Aged Rats

scholarly journals Synergistic Effects of Zn, Cu, and Ni and Bacillus Thuringiensis On the Hemocyte Count and the Antioxidant Activities of Hyphantria Cunea Drury (Lepidoptera: Arctiidae) Larvae

2021 ◽  
Author(s):  
Oğuzhan Yanar ◽  
Elif F. Topkara ◽  
Fatma G. Solmaz ◽  
Sevcan Mercan
Keyword(s):  
Superoxide Dismutase ◽  
Bacillus Thuringiensis ◽  
Glutathione Peroxidase ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Activities ◽  
Model Organisms ◽  
Metal Exposure ◽  
Antioxidant Enzyme Activities ◽  
Hyphantria Cunea

Abstract Insects are model organisms for immunological studies. The cellular and the antioxidant enzyme responses of insects are major bioindicators against environmental stresses (metal exposure, infection, etc.). In our study, the differences in the hemocyte counts and the antioxidant enzyme activities of Hyphantria cunea larvae exposed to the different amounts of zinc, copper, and nickel and Bacillus thuringiensis infection were determined. With metal exposure, the superoxide dismutase, catalase, and glutathione peroxidase activities increased, but the hemocyte counts decreased. Additionally, both the hemocyte counts and the enzyme activities increased with Bacillus thuringiensis infection. As a result of this study, we found that the superoxide dismutase, catalase, and glutathione peroxidase and the hemocyte counts varied in response to both metal exposure and bacterial infection.

Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression

1988 ◽  
Vol 8 (11) ◽  
pp. 4851-4857
Author(s):  
P Bornstein ◽  
J McKay ◽  
D J Liska ◽  
S Apone ◽  
S Devarayalu
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Human Growth ◽  
Collagen Gene ◽  
Mrna Levels ◽  
3T3 Cells ◽  
Rnase Protection ◽  
First Intron

The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblasts and NIH 3T3 cells. In chick tendon fibroblasts, but not in 3T3 cells, inversion of intronic sequences containing a previously studied 274-base-pair segment, A274, resulted in markedly reduced human growth hormone mRNA levels as determined by an RNase protection assay. This inhibitory effect was largely alleviated when deletions were introduced in the collagen promoter of plasmids containing negatively oriented intronic sequences. Evidence for interaction of the promoter with the intronic segment, A274, was obtained by gel mobility shift assays. We suggest that promoter-intron interactions, mediated by DNA-binding proteins, regulate collagen gene transcription. Inversion of intronic segments containing critical interactive elements might then lead to an altered geometry and reduced activity of a transcriptional complex in those cells with sufficiently high levels of appropriate transcription factors. We further suggest that the deleted promoter segment plays a key role in directing DNA interactions involved in transcriptional control.

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