scholarly journals Cationic Porphyrin Induced a Telomeric DNA to G-Quadruplex Form in Water

2008 ◽  
Vol 2008 ◽  
pp. 1-5 ◽  
Author(s):  
Takashi Murashima ◽  
Daichi Sakiyama ◽  
Daisuke Miyoshi ◽  
Masanori Kuriyama ◽  
Takashi Yamada ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Circular Dichroism Spectroscopy ◽  
Telomeric Repeat ◽  
Telomeric Dna ◽  
Cationic Porphyrin ◽  
G Quadruplex ◽  
Circular Dichroïsm

The formation of the DNA G-quadruplex is induced by the addition of a novel porphyrin carrying four cationic tethers. Circular dichroism spectroscopy reveals that the porphyrin binds toTetrahymenatelomeric repeat to form G-quadruplex under stabilizing-cation-deficient and no buffer conditions.

THE INFLUENCE OF UREA ON G-QUADRUPLEX AND i-MOTIF STRUCTURES IN COMPLEMENTARY DNA SEQUENCES

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 115-122
Author(s):  
Ye.B. Dalyan ◽  
L.G. Aslanyan ◽  
I.V. Vardanyan
Keyword(s):  
Circular Dichroism ◽  
Dna Sequences ◽  
Thermal Denaturation ◽  
Temperature Scale ◽  
Single Strand ◽  
Structural Transitions ◽  
Telomeric Dna ◽  
Complementary Dna ◽  
G Quadruplex ◽  
Circular Dichroïsm

In the present study, the methods of circular dichroism and UV/Vis spectrophotometry were used to study the influence of urea on the structural transitions i-motif $\leftrightarrows$ unfolded single strand in cytosine-rich ${\text{d[3}^{\prime}\text{-(CCCAAT)}_{3}\text{CCC-5)}^{\prime}]}$ region of telomeric DNA (Tel22C) and G-quadruplex $\leftrightarrows$ unfolded single strand in complementary guanine-rich strand ${\text{d[5}^{\prime}\text{-A(GGGTTA)}_{3}\text{GGG-3}^{\prime}]}$ (Tel22G) at pH 5.5 and 400 mM Na+. Under these conditions, Tel22C and Tel22G were found to form stable i-motif and G-quadruplex structures. It has been shown that urea (0-8 M) destabilizes the i-motif and G-quadruplex structures, but unlike thermal denaturation, it does not destroy the structures completely. The melting processes of G-quadruplex and i-motif are separated in the temperature scale (at any concentration of urea, the melting of the G-quadruplex starts at temperatures where the melting of the i-motifs has already been completed).

scholarly journals Interaction of 9-Methoxyluminarine with Different G-Quadruplex Topologies: Fluorescence and Circular Dichroism Studies

2021 ◽  
Vol 22 (19) ◽  
pp. 10399
Author(s):  
Joanna Nowak-Karnowska ◽  
Agata Głuszyńska ◽  
Joanna Kosman ◽  
Grażyna Neunert ◽  
Anna Dembska
Keyword(s):  
Circular Dichroism ◽  
Molecular Targets ◽  
Circular Dichroism Spectroscopy ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Circular Dichroïsm

The interactions of G–quadruplexes of different topologies with highly fluorescent 9-methoxyluminarine ligand 9-MeLM were investigated by fluorescence and circular dichroism spectroscopy. The results showed that 9-methoxyluminarine was able to interact and did not destabilize any investigated molecular targets. The studied compound was selectively quenched by parallel c-MYC G-quadruplex DNA, whereas hybrid and antiparallel G4 topology caused only a negligible decrease in the fluorescence of the ligand. A high decrease of fluorescence of the ligand after binding with c-MYC G-quadruplex suggests that this molecule can be used as a selective probe for parallel G-quadruplexes.

scholarly journals G-Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy

2018 ◽  
Vol 57 (24) ◽  
pp. 7171-7175 ◽  
Author(s):  
Rafael del Villar-Guerra ◽  
John O. Trent ◽  
Jonathan B. Chaires
Keyword(s):  
Circular Dichroism ◽  
Secondary Structure ◽  
Circular Dichroism Spectroscopy ◽  
G Quadruplex ◽  
Circular Dichroïsm

G-Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy

2018 ◽  
Vol 130 (24) ◽  
pp. 7289-7293 ◽  
Author(s):  
Rafael del Villar-Guerra ◽  
John O. Trent ◽  
Jonathan B. Chaires
Keyword(s):  
Circular Dichroism ◽  
Secondary Structure ◽  
Circular Dichroism Spectroscopy ◽  
G Quadruplex ◽  
Circular Dichroïsm
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