scholarly journals Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Iwona Jedrzejczyk ◽  
Elwira Sliwinska
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Woody Species ◽  
Size Estimation ◽  
Flow Cytometric ◽  
Cell Cytosol ◽  
High Concentrations ◽  
First Time

The presence of some secondary metabolites in the cell cytosol can cause a stoichiometric error in the flow cytometric estimation of nuclear DNA content. There is no fully reliable method to completely eliminate the effect of these compounds on nuclei fluorescence, and therefore using plant organs/parts free of staining inhibitors is recommended. Eleven species of Rosaceae with high concentrations of propidium-iodide-staining inhibitors were studied to check the possibility of using seeds instead of leaves for genome size estimation. Despite optimizing the concentration and composition of antioxidants in nuclei-isolation buffer for each species, the effect of cytosolic compounds present in the leaves could not be avoided entirely. None of the seeds of the studied species contained inhibitors, and they produced histograms of good quality. The genome size of the studied species ranged from 1.15 to 3.17 pg/2C; for 10 species the DNA content was estimated for the first time.

Peaceful revolution in genome size: polyploidy in the Nabidae (Heteroptera); autosomes and nuclear DNA content doubling

2020 ◽  
Author(s):  
David Sadílek ◽  
Jitka Vilímová ◽  
Tomáš Urfus
Keyword(s):  
Genome Size ◽  
X Chromosome ◽  
Dna Content ◽  
18S Rdna ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome Breakage ◽  
Polyploidy Event ◽  
First Time

Abstract Genome size and the position of 18S ribosomal DNA (rDNA) were analysed in two Himacerus, eight Nabis and two Prostemma species from the family Nabidae using flow cytometry and fluorescence in situ hybrization techniques. The karyotypes of Nabis biformis and Nabis maoricus, each with 2n = 16 + XY, and Prostemma aeneicolle, with 2n = 26 + XY, were recorded for the first time. All the species displayed one or two 18S rDNA signals on the X chromosome and up to two signals on the Y chromosome. Several females exhibited two different types of X chromosome breakage, namely within or outside of the 18S rDNA region. Measurements of nuclear DNA content revealed significant differences between all three genera under study. Most notably, the nuclear DNA content of Himacerus species, with 2n = 32/36 + XY (2C = 9–10 pg), was double that of Nabis species, with 2n = 16 + XY (2C = 4–6 pg). Therefore, the previously rejected theory of an autosomal polyploidy event in the evolution of the genus Himacerus is strongly supported by the results of the present study and is now being resurrected.

First report of genome size and ploidy of the underutilized leguminous tuber crop Yam Bean (Pachyrhizus erosus and P. tuberosus) by flow cytometry

2019 ◽  
Vol 17 (5) ◽  
pp. 456-459 ◽  
Author(s):  
Kalidas Pati ◽  
Fangning Zhang ◽  
Jacqueline Batley
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
First Report ◽  
Flow Cytometric ◽  
Pachyrhizus Erosus ◽  
Tuber Crop

AbstractCytological understanding is an important parameter to understand the genetic architecture of yam bean. The ploidy level and genome size of two cultivated species of yam bean (Pachyrhizus erosus and P. tuberosus) were estimated using flow cytometric analysis of young leaf tissue, with propidium iodide as a fluorescent dye. Six genotypes of P. erosus and three genotypes of P. tuberosus were analysed. Rice (Oryza sativa cv. Nipponbare) and Mung bean (Vigna radiata cv Berken) were used as internal reference standards. Variation of 2C nuclear DNA content among the six P. erousus lines was 4.18%, ranging from 1.17 to 1.22 pg, whereas only 1.8% variation was observed among the three P. tuberosus lines, which ranged from 1.07 to 1.09 pg. Moreover, it was found that the nuclear DNA content of P. tuberosus was lower than that of P. erosus. The result of the flow cytometric analysis showed that all the species were diploid (2n = 2x) and coefficient of variation (CV%) of all the accessions of the two species was less than 3.5%. This is the first report of ploidy analysis and genome size estimation of the leguminous underutilized tuber crop yam bean using flow cytometry. This result will be helpful for yam bean genome sequencing and crop improvement programmes.

Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae

1988 ◽  
Vol 7 (1) ◽  
pp. 74-77 ◽  
Author(s):  
Jan Fahleson ◽  
Johan Dixelius ◽  
Eva Sundberg ◽  
Kristina Glimelius
Keyword(s):  
Chromosome Number ◽  
Dna Content ◽  
Nuclear Dna ◽  
Somatic Hybrids ◽  
Nuclear Dna Content ◽  
Flow Cytometric

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

scholarly journals Miscanthus: Inter- and Intraspecific Genome Size Variation Among M. × Giganteus, M. Sinensis, M. Sacchariflorus Accessions

2015 ◽  
Vol 57 (1) ◽  
pp. 104-113
Author(s):  
Sandra Cichorz ◽  
Maria Gośka ◽  
Monika Rewers
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
Nuclear Dna ◽  
Size Variation ◽  
Nuclear Dna Content ◽  
Interspecific Variation ◽  
Genome Size Variation ◽  
2C Dna Content ◽  
Cytological Characteristics ◽  
Stomatal Length

AbstractSinceM. sinensisAnderss.,M. sacchariflorus(Maxim.) Hack. andM. ×giganteusJ.M.Greef & Deuter ex Hodk. and Renvoize have considerably the highest potential for biomass production amongMiscanthusAnderss. species, there is an urgent need to broaden the knowledge about cytological characteristics required for their improvement. In this study our objectives were to assess the genome size variation among eighteenMiscanthusaccessions, as well as estimation of the monoploid genome size (2C and Cx) of theM. sinensiscultivars, which have not been analyzed yet. The characterization of threeMiscanthusspecies was performed with the use of flow cytometry and analysis of the stomatal length. The triploid (2n = 3x = 57)M. sinensis‘Goliath’ andM. ×giganteusclones possessed the highest 2C DNA content (8.34 pg and 7.43 pg, respectively). The intermediate 2C-values were found in the nuclei of the diploid (2n = 2x = 38)M. sinensisaccessions (5.52–5.72 pg), whereas they were the lowest in the diploid (2n = 2x = 38)M. sacchariflorusecotypes (4.58–4.59 pg). The presented study revealed interspecific variation of nuclear DNA content (P<0.01) and therefore allowed for recognition of particular taxa, inter- and intraspecific hybrids and prediction of potential parental components. Moreover, intraspecific genome size variation (P<0.01) was observed inM. sinensiscultivars at 3.62%. The values of the stomatal size obtained for the triploidM. ×giganteus‘Great Britain’ (mean 30.70 μm) or ‘Canada’ (mean 29.67 μm) and diploidM. sinensis‘Graziella’ (mean 29.96 μm) did not differ significantly, therefore this parameter is not recommended for ploidy estimation.

Flow cytometric measurement of nuclear DNA content variations as a potentialin vivo mutagenicity test

Cytometry ◽  
1981 ◽  
Vol 2 (3) ◽  
pp. 189-191 ◽  
Author(s):  
F. J. Otto ◽  
H. Oldiges ◽  
W. Göhde ◽  
V. K. Jain
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Mutagenicity Test ◽  
Flow Cytometric ◽  
Flow Cytometric Measurement

scholarly journals Reliable flow cytometric estimation of nuclear DNA content in coffee trees

Cytometry ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Philippe Barre ◽  
Michel Noirot ◽  
Jacques Louarn ◽  
Christophe Duperray ◽  
Serge Hamon
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Flow Cytometric
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