scholarly journals Incidence of Malaria in the Interior Division of Sabah, Malaysian Borneo, Based on Nested PCR

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Wan Fen Joveen-Neoh ◽  
Ka Lung Chong ◽  
Clemente Michael Vui Ling Wong ◽  
Tiek Ying Lau
Keyword(s):  
Ribosomal Rna ◽  
Nested Pcr ◽  
Malaria Parasite ◽  
Molecular Detection ◽  
Small Subunit ◽  
Blood Film ◽  
Malaria Parasites ◽  
Human Malaria ◽  
Blood Spot ◽  
Malaysian Borneo

Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genusPlasmodium. Misidentification of human malaria parasites especiallyP. knowlesibased on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites.Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study.Results. Nested PCR findings showed that there was noP. malariaeinfection while the highest prevalent malaria parasite wasP. knowlesi, followed byP. vivax,P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results.Conclusion. The preliminary findings from molecular detection of malaria showed thatP. knowlesiwas the most prevalentPlasmodiumspecies in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of differentPlasmodiumspecies in this region.

Blood and sporozoite stage-specific small subunit ribosomal RNA-encoding genes of the human malaria parasite Plasmodium vivax

Gene ◽  
1994 ◽  
Vol 150 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Shoukat H. Qar ◽  
Ira F. Goldman ◽  
Norman J. Pieniazek ◽  
William E. Collins ◽  
Altaf A. Lal
Keyword(s):  
Plasmodium Vivax ◽  
Ribosomal Rna ◽  
Malaria Parasite ◽  
Small Subunit ◽  
Human Malaria Parasite ◽  
Human Malaria ◽  
Small Subunit Ribosomal Rna ◽  
Parasite Plasmodium ◽  
Encoding Genes

Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene

1997 ◽  
Vol 46 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Masatsugu Kimura ◽  
Osamu Kaneko ◽  
Qing Liu ◽  
Mian Zhou ◽  
Fumihiko Kawamoto ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Malaria Parasites ◽  
Human Malaria ◽  
Small Subunit Rrna Gene

scholarly journals A cornucopia of research resources for the fourth rodent malaria parasite species

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jane M. Carlton
Keyword(s):  
Malaria Parasite ◽  
Parasite Species ◽  
First Century ◽  
Model Systems ◽  
Rodent Malaria Parasite ◽  
Malaria Parasites ◽  
Rodent Malaria ◽  
Human Malaria ◽  
Plasmodium Vinckei ◽  
Research Resources

AbstractThe study of human malaria caused by species of Plasmodium has undoubtedly been enriched by the use of model systems, such as the rodent malaria parasites originally isolated from African thicket rats. A significant gap in the arsenal of resources of the species that make up the rodent malaria parasites has been the lack of any such tools for the fourth of the species, Plasmodium vinckei. This has recently been rectified by Abhinay Ramaprasad and colleagues, whose pivotal paper published in BMC Biology describes a cornucopia of new P. vinckei ‘omics datasets, mosquito transmission experiments, transfection protocols, and virulence phenotypes, to propel this species firmly into the twenty-first century.

scholarly journals Molecular Detection and Characterization of Goat Isolate ofTaenia hydatigenain Turkey

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Armagan Erdem Utuk ◽  
Fatma Cigdem Piskin
Keyword(s):  
Ribosomal Rna ◽  
Molecular Detection ◽  
Sequence Data ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Molecular Tools ◽  
Taenia Hydatigena ◽  
Nucleotide Sequence Data ◽  
First Time

The aim of this study was to provide molecular detection and characterization of the goat isolate ofTaenia hydatigenafrom Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified asTaeniaspp., and partial sequence of mt-CO1 gene was corresponding toT. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate ofT. hydatigenawas submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization ofT. hydatigenawas done for the first time in Turkey.

scholarly journals New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi

2020 ◽  
Author(s):  
Joshua Ang Xin De ◽  
Khamisah Abdul Kadir ◽  
Dayang Shuaisah Awang Mohamad ◽  
Asmad Matusop ◽  
Paul Cliff Simon Divis ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Malaria Parasite ◽  
Plasmodium Knowlesi ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Malaria Vectors ◽  
Zoonotic Malaria ◽  
Parasite Plasmodium ◽  
Malaysian Borneo

Abstract BackgroundThe vectors for Plasmodium knowlesi, a significant cause of human malaria in Southeast Asia, identified previously in nature all belong to the Anopheles Leucosphyrus Group. Only one study has been previously undertaken in Sarawak, Malaysian Borneo, to identify vectors of P. knowlesi, where Anopheles latens was incriminated as the vector in Kapit, central Sarawak. A study was therefore undertaken to identify malaria vectors in a different location in Sarawak. MethodsMosquitoes found landing on humans and resting on leaves over a 5-day period at two sites in the Lawas District of northern Sarawak were collected and identified. DNA samples extracted from salivary glands of anophelines were subjected to nested PCR malaria-detection assays. The small sub-unit ribosomal RNA (SSUrRNA) genes of Plasmodium, and the internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) sequences of the mosquitoes were derived from the Plasmodium-positive samples for phylogenetic analyses. ResultsA total of 65 anophelines and 127 culicines were collected. By PCR, six An. balabacensis and five An. barbirostris were found to have single P. knowlesi infecions while three other An. balabacensis had either single, double or triple infections with P. inui, P. fieldi, P. cynomolgi and P. knowlesi. Phylogenetic analyses of the Plasmodium SSUrRNA genes confirmed 3 An. barbirostris and 3 An. balabacensis with single P. knowlesi infections, while 3 other An. balabacensis had two or more Plasmodium species of P. inui, P. knowlesi, P. cynomolgi and possibly novel species of Plasmodium. Phylogenies inferred from the ITS2 and CO1 sequences of An. balabacensis and An. barbirostris indicate that the former is genetically indistinguishable from An. balabacensis in Borneo while the latter is a novel sibling species belonging to the Anopheles Barbirostris Subgroup. ConclusionsNew vectors for P. knowlesi in Sarawak were identified, including An. barbirostris, which is a species that does not belong to the Anopheles Leucosphyrus Group.

Generation of Transgenic Rodent Malaria Parasites Expressing Human Malaria Parasite Proteins

2015 ◽  
pp. 257-286 ◽  
Author(s):  
Ahmed M. Salman ◽  
Catherin Marin Mogollon ◽  
Jing-wen Lin ◽  
Fiona J. A. van Pul ◽  
Chris J. Janse ◽  
...  
Keyword(s):  
Malaria Parasite ◽  
Malaria Parasites ◽  
Human Malaria Parasite ◽  
Rodent Malaria ◽  
Human Malaria

scholarly journals Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium

2013 ◽  
Vol 58 (No. 10) ◽  
pp. 535-542 ◽  
Author(s):  
MCM Couto ◽  
AP Sudre ◽  
MF Lima ◽  
TCB Bomfim
Keyword(s):  
Dna Extraction ◽  
Ethidium Bromide ◽  
Ribosomal Rna ◽  
Nested Pcr ◽  
Extraction Methods ◽  
Small Subunit ◽  
Molecular Techniques ◽  
Comparison Of Techniques ◽  
Low Toxicity ◽  
Dna Extraction Methods

Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. However, if these tools are to be used correctly and accurately, the techniques used must be standardised. In the present study, two molecular techniques for diagnosing Cryptosporidium infection in cows were compared to determine the optimal methods. For each technique, we tested two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA), and the GP60 (60 kDa glycoprotein) genes, and two types of DNA staining reagents, ethidium bromide and GelRed<sup>TM</sup>. We determined that one of the tested protocols yields a higher purity of extracted DNA. Additionally, optimised temperatures for the nested PCR of the 18S and GP60 genes were established. Finally, we determined that the GelRed<sup>TM</sup> dye was more sensitive than ethidium bromide, and its low toxicity facilitates handling and disposal and reduces environmental contamination.

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