scholarly journals Is There Any Relationship between Human Herpesvirus-8 and Multiple Myeloma?

Lymphoma ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Mohammad Hadi Sadeghian ◽  
Maryam Mohammadnia Avval ◽  
Hossein Ayatollahi ◽  
Mohammad Reza Keramati ◽  
Bahram Memar ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Plasma Cells ◽  
Human Herpesvirus ◽  
The Body ◽  
Human Herpesvirus 8 ◽  
Bone Marrow Biopsies ◽  
Exact Test ◽  
Herpesvirus 8

Background. Human herpesvirus-8 (HHV-8) is associated with some human diseases including Kaposi’s sarcoma and also some B-cell lymphoproliferative disorders. Few studies have highlighted the potential role of HHV-8 in the development of multiple myeloma (MM) which is known as a malignant proliferation of plasma cells derived from a single clone. Aims. The aim of this study was to find a relationship between HHV-8 and MM using polymerase chain reaction (PCR) method. Materials and Methods. This study was conducted on 30 formalin-fixed, paraffin-embedded (FFPE) bone marrow biopsies of multiple myeloma and 30 normal FFPE bone marrow biopsies. After the sample preparation, Deoxyribonucleic acid (DNA) was extracted by nonheating procedure. PCR for HHV-8 virus was carried out with commercial kit and the PCR products were visualized by gel electrophoresis. Finally, the statistical analysis was performed. Results. HHV-8 virus was not detected by PCR from FFPE blocks of multiple myeloma samples, while only one of the controls showed DNA band of the corrected molecular weights. Fisher’s exact test showed that no statistical differences were found between the two groups (P=0.999). Conclusion. Our report adds to the body of evidence that there is no association between HHV- 8 and MM against a major role of HHV-8 infection in the pathogenesis of clonal plasma cell proliferation.

Detection of Human Herpesvirus-8 DNA in Bone Marrow Biopsies From Patients With Multiple Myeloma and Waldenström's Macroglobulinemia

Blood ◽  
1998 ◽  
Vol 91 (11) ◽  
pp. 4393-4394 ◽  
Author(s):  
Félix Agbalika ◽  
Xavier Mariette ◽  
Jean-Pierre Marolleau ◽  
Jean-Paul Fermand ◽  
Jean-Claude Brouet
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Bone Marrow Biopsies ◽  
Herpesvirus 8

Detection of Human Herpesvirus-8 DNA in Bone Marrow Biopsies From Patients With Multiple Myeloma and Waldenström's Macroglobulinemia

Blood ◽  
1998 ◽  
Vol 91 (11) ◽  
pp. 4393-4394 ◽  
Author(s):  
Félix Agbalika ◽  
Xavier Mariette ◽  
Jean-Pierre Marolleau ◽  
Jean-Paul Fermand ◽  
Jean-Claude Brouet
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Bone Marrow Biopsies ◽  
Herpesvirus 8

Low Incidence of Human Herpesvirus 8 in Bone Marrow Samples from Brazilian Patients with Multiple Myeloma

2001 ◽  
Vol 105 (4) ◽  
pp. 247-248 ◽  
Author(s):  
A. Cunha ◽  
S.C. Costa ◽  
C.S.P. Lima ◽  
M. Ortega ◽  
F.F. Costa
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Low Incidence

Molecular and Serological Examination of the Relationship of Human Herpesvirus 8 to Multiple Myeloma: orf 26 Sequences in Bone Marrow Stroma Are Not Restricted to Myeloma Patients and Other Regions of the Genome Are Not Detected

Blood ◽  
1998 ◽  
Vol 92 (8) ◽  
pp. 2681-2687 ◽  
Author(s):  
John F. Tisdale ◽  
A. Keith Stewart ◽  
Bruce Dickstein ◽  
Richard F. Little ◽  
Ian Dubé ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Limit Of Detection ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Human Control ◽  
Control Subjects ◽  
Control Samples

Abstract Human herpesvirus 8 (HHV-8) genomic sequences were recently detected by polymerase chain reaction (PCR) and in situ hybridization in bone marrow stromal cells grown from multiple myeloma (MM) patients, but not in cells from control subjects (Rettig et al, Science 276:1851, 1997). We sought to confirm these observations in our own group of MM patients (n = 30). DNA was extracted from adherent stromal cells grown under varying conditions and assayed for HHV-8 sequence using PCR to amplify the orf 26 (KS330) sequence (Chang et al,Science 266:1865, 1997), as initially reported. Samples from human control subjects (n = 25) were concurrently extracted and analyzed. After 30 cycles of amplification, we did not detect any positive samples. In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were positive, at about the limit of detection, but orf 26 sequence was also amplified from 11 of 25 (44%) human control samples. However, PCR amplification from other regions of the viral genome (orf 72 and orf 75) was uniformly negative for all MM and control samples, despite equivalent sensitivity. Additionally, all sera from MM patients were negative for HHV-8 IgG by immunofluorescence. Our data do not support a role of HHV-8 in the etiology of MM but may suggest the presence of a related (KS330-containing) virus in MM patients and in some control subjects. This is a US government work. There are no restrictions on its use.

Molecular and Serological Examination of the Relationship of Human Herpesvirus 8 to Multiple Myeloma: orf 26 Sequences in Bone Marrow Stroma Are Not Restricted to Myeloma Patients and Other Regions of the Genome Are Not Detected

Blood ◽  
1998 ◽  
Vol 92 (8) ◽  
pp. 2681-2687 ◽  
Author(s):  
John F. Tisdale ◽  
A. Keith Stewart ◽  
Bruce Dickstein ◽  
Richard F. Little ◽  
Ian Dubé ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Limit Of Detection ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Human Control ◽  
Control Subjects ◽  
Control Samples

Human herpesvirus 8 (HHV-8) genomic sequences were recently detected by polymerase chain reaction (PCR) and in situ hybridization in bone marrow stromal cells grown from multiple myeloma (MM) patients, but not in cells from control subjects (Rettig et al, Science 276:1851, 1997). We sought to confirm these observations in our own group of MM patients (n = 30). DNA was extracted from adherent stromal cells grown under varying conditions and assayed for HHV-8 sequence using PCR to amplify the orf 26 (KS330) sequence (Chang et al,Science 266:1865, 1997), as initially reported. Samples from human control subjects (n = 25) were concurrently extracted and analyzed. After 30 cycles of amplification, we did not detect any positive samples. In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were positive, at about the limit of detection, but orf 26 sequence was also amplified from 11 of 25 (44%) human control samples. However, PCR amplification from other regions of the viral genome (orf 72 and orf 75) was uniformly negative for all MM and control samples, despite equivalent sensitivity. Additionally, all sera from MM patients were negative for HHV-8 IgG by immunofluorescence. Our data do not support a role of HHV-8 in the etiology of MM but may suggest the presence of a related (KS330-containing) virus in MM patients and in some control subjects. This is a US government work. There are no restrictions on its use.

scholarly journals Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM)

Leukemia ◽  
2001 ◽  
Vol 15 (8) ◽  
pp. 1268-1273 ◽  
Author(s):  
M Beksac ◽  
M Ma ◽  
C Akyerli ◽  
M DerDanielian ◽  
L Zhang ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Bone Marrow Biopsy ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
Turkish Patients

THE ROLE OF HUMAN HERPESVIRUS-8 IN THE PATHOGENESIS OF MULTIPLE MYELOMA

1999 ◽  
Vol 13 (6) ◽  
pp. 1159-1167 ◽  
Author(s):  
Nelida N. Sjak-Shie ◽  
Robert A. Vescio ◽  
James R. Berenson
Keyword(s):  
Multiple Myeloma ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8
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