Evaluation of a New Lipase fromStaphylococcussp. for Detergent Additive Capability

Lipases are the enzymes of choice for laundry detergent industries owing to their triglyceride removing ability from the soiled fabric which eventually reduces the usage of phosphate-based chemical cleansers in the detergent formulation. In the present study, a partially purified bacterial lipase fromStaphylococcus arlettaeJPBW-1 isolated from the rock salt mine has been assessed for its triglyceride removing ability by developing a presoak solution so as to use lipase as an additive in laundry detergent formulations. The effects of selected surfactants, commercial detergents, and oxidizing agents on lipase stability were studied in a preliminary evaluation for its further usage in the industrial environment. Partially purified lipase has shown good stability in presence of surfactants, commercial detergents, and oxidizing agents. Washing efficiency has been found to be enhanced while using lipase with 0.5% nonionic detergent than the anioinic detergent. The wash performance using 0.5% wheel with 40 U lipase at 40°C in 45 min results in maximum oil removal (62%) from the soiled cotton fabric. Hence, the present study opens the new era in enzyme-based detergent sector for formulation of chemical-free detergent using alkaline bacterial lipase.

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Potential of a Novel Thermotolerant Lipase Bacillus stearothermophilus nr22 (Lip.nr-22) as Additive in High Temperature Operated-Neutral pH Liquid Detergent

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.735.136 ◽

2017 ◽

Vol 735 ◽

pp. 136-142 ◽

Cited By ~ 1

Author(s):

Nik Raikhan Nik Him ◽

Nurul Shafika Azmi

Keyword(s):

High Temperature ◽

Bacillus Stearothermophilus ◽

Laundry Detergent ◽

Detergent Formulation ◽

Time Interval ◽

Oil Removal ◽

Optimum Conditions ◽

Detergent Concentration ◽

Neutral Ph ◽

Commercial Detergents

Enzyme-added detergent must have the capability to operate at high temperature to support the enzyme proteins to clean soiled-fabrics at optimum conditions. Lipase from Bacillus stearothermophilus nr22 (Lip.nr-22) has improved the oil removal from soiled-cotton fabric by 38.8-51.4% in 4 types of local commercial detergents. The later was the oil removal from an unrevealed detergent. The optimum conditions were 108U/ml Lip.nr-22 in 0.1M, pH 7.0, washing temperature and washing time interval as 80°C and 40 min, respectively; shaking wash at 300 rpm and percentage of detergent concentration as 0.5. Lip.nr-22 is a very potential enzyme in high temperature-neutral pH operated laundry detergent formulations. It has exhibited a very excellent thermostability at 80°C, was very stable with surfactants, commercial detergents as well as with oxidizing agents (H2O2, NaBO3H2O and NaClO). Lip.nr-22 as additive in detergent formulation is a promise for better detergent formulation.

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Production of an oxidant and SDS-stable alkaline protease from an alkaophilic Bacillus clausii I-52 by submerged fermentation: Feasibility as a laundry detergent additive

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2005.05.008 ◽

2006 ◽

Vol 38 (1-2) ◽

pp. 176-183 ◽

Cited By ~ 21

Author(s):

Han-Seung Joo ◽

Chung-Soon Chang

Keyword(s):

Alkaline Protease ◽

Submerged Fermentation ◽

Laundry Detergent ◽

Detergent Additive ◽

Bacillus Clausii

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Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive

Process Biochemistry ◽

10.1016/s0032-9592(99)00053-9 ◽

1999 ◽

Vol 35 (1-2) ◽

pp. 213-219 ◽

Cited By ~ 119

Author(s):

Uttam Chand Banerjee ◽

Rajesh Kumar Sani ◽

Wamik Azmi ◽

Raman Soni

Keyword(s):

Alkaline Protease ◽

Laundry Detergent ◽

Detergent Additive ◽

Bacillus Brevis

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Albacore tuna spleen trypsin: Potential application as laundry detergent additive and in carotenoprotein extraction from Pacific white shrimp shells

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2019.01.013 ◽

2019 ◽

Vol 17 ◽

pp. 638-646 ◽

Cited By ~ 1

Author(s):

Tanchanok Poonsin ◽

Benjamin K. Simpson ◽

Soottawat Benjakul ◽

Wonnop Visessanguan ◽

Asami Yoshida ◽

...

Keyword(s):

Potential Application ◽

Laundry Detergent ◽

Pacific White Shrimp ◽

White Shrimp ◽

Detergent Additive ◽

Albacore Tuna ◽

Shrimp Shells

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Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: Purification, characterization and potential application as a laundry detergent additive

Bioresource Technology ◽

10.1016/j.biortech.2009.01.061 ◽

2009 ◽

Vol 100 (13) ◽

pp. 3366-3373 ◽

Cited By ~ 167

Author(s):

Anissa Haddar ◽

Rym Agrebi ◽

Ali Bougatef ◽

Noomen Hmidet ◽

Alya Sellami-Kamoun ◽

...

Keyword(s):

Potential Application ◽

Serine Proteases ◽

Laundry Detergent ◽

Detergent Additive ◽

Bacillus Mojavensis

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An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-009-8703-z ◽

2009 ◽

Vol 160 (8) ◽

pp. 2308-2321 ◽

Cited By ~ 38

Author(s):

Laila Manni ◽

Kemel Jellouli ◽

Olfa Ghorbel-Bellaaj ◽

Rym Agrebi ◽

Anissa Haddar ◽

...

Keyword(s):

Bacillus Cereus ◽

Laundry Detergent ◽

Detergent Additive ◽

Shrimp Wastes ◽

Solvent Stable

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Laundry detergent additive containing zeolite, polycarboxylate, and perborate

Zeolites ◽

10.1016/0144-2449(91)80394-f ◽

1991 ◽

Vol 11 (1) ◽

pp. 90 ◽

Cited By ~ 3

Author(s):

H. Upadek ◽

E. Smulders ◽

J. Poethkow

Keyword(s):

Laundry Detergent ◽

Detergent Additive

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Microbial Proteases and Application as Laundry Detergent Additive

Research Journal of Microbiology ◽

10.3923/jm.2008.661.672 ◽

2008 ◽

Vol 3 (12) ◽

pp. 661-672 ◽

Cited By ~ 47

Author(s):

D. Kumar ◽

Savitri . ◽

N. Thakur ◽

R. Verma ◽

T. C. Bhalla

Keyword(s):

Laundry Detergent ◽

Detergent Additive

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A Purified Alkaline and Detergent-Tolerant Lipase from Aspergillus fumigatus with Potential Application in Removal of Mustard Oil Stains from Cotton Fabric

Tenside Surfactants Detergents ◽

10.1515/tsd-2021-2378 ◽

2021 ◽

Vol 58 (6) ◽

pp. 442-451

Author(s):

Akshita Mehta ◽

Aashna Gupta ◽

Kamal Kumar Bhardwaj ◽

Reena Gupta

Keyword(s):

Hydrogen Peroxide ◽

Aspergillus Fumigatus ◽

Cotton Fabric ◽

Tween 80 ◽

Maximum Activity ◽

Mustard Oil ◽

Oil Removal ◽

Oxidizing Agents ◽

Wash Temperature ◽

Optimized Conditions

Abstract In the present study, the lipase from Aspergillus fumigatus was purified which was found to be stable to commercial detergents and oxidising agents. A purification fold of 6.96 and yield of 11.03% were achieved when the enzyme was purified using Octyl Sepharose column chromatography. In presence of various oxidizing agents, the highest activity of lipase was 15.56 U/mg with hydrogen peroxide. Among various surfactants used, the maximum activity exhibited by lipase was with Tween 80. While studying the effect of various detergents, the highest activity of 9.3 U/mg was achieved with "Vanish" detergent. Wash performance was studied with various detergents out of which "Vanish" showed highest oil removal of 79%. Lipase from Aspergillus fumigatus possessed better stability with various surfactants and oxidizing agents. The results of this study have shown that the lipase from Aspergillus fumigatus along with detergent "Vanish" (0.7%) under optimized conditions (5 μg/ml lipase, 40°C wash temperature and 40 min wash duration) improved oil removal from cotton fabric stained with mustard oil by 84%.

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