Nucleotide Sequencing and Identification of Some Wild Mushrooms

The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned. The sequences were aligned using ClustalW software program. The aligned sequences revealed identity (homology percentage from GenBank data base) ofAmanita hemibapha[CN (Chota Nagpur) 1, % identity 99 (JX844716.1)],Amanitasp. [CN 2, % identity 98 (JX844763.1)],Astraeus hygrometricus[CN 3, % identity 87 (FJ536664.1)],Termitomycessp. [CN 4, % identity 90 (JF746992.1)],Termitomycessp. [CN 5, % identity 99 (GU001667.1)],T. microcarpus[CN 6, % identity 82 (EF421077.1)],Termitomycessp. [CN 7, % identity 76 (JF746993.1)], andVolvariella volvacea[CN 8, % identity 100 (JN086680.1)]. Although out of 8 mushrooms 4 could be identified up to species level, the nucleotide sequences of the rest may be relevant to further characterization. A phylogenetic tree is constructed using Neighbor-Joining method showing interrelationship between/among the mushrooms. The determined nucleotide sequences of the mushrooms may provide additional information enriching GenBank database aiding to molecular taxonomy and facilitating its domestication and characterization for human benefits.