scholarly journals Iron Oxide Magnetic Nanoparticles: Characterization and Toxicity Evaluation byIn VitroandIn VivoAssays

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Alina Mihaela Prodan ◽  
Simona Liliana Iconaru ◽  
Carmen Steluta Ciobanu ◽  
Mariana Carmen Chifiriuc ◽  
Mihai Stoicea ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hela Cells ◽  
Intraperitoneal Injection ◽  
Colorimetric Assay ◽  
Brown Norway ◽  
Oxide Nanoparticles

The aim of this study was to evaluate the biological properties of iron oxide nanoparticles (IO-NPs) obtained in the aqueous suspension. The iron oxide nanoparticles were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The biocompatibility of the iron oxide was demonstrated by thein vitroquantification of HeLa cells viability using propidium iodide (PI) and fluorescein diacetate (FdA) and the MTT colorimetric assay. The toxicity of small size iron oxide nanoparticles was also evaluated by means of histological examination on male Brown Norway rats after intraperitoneal injection. At the tested concentrations, the nanoparticles proved to be not cytotoxic on HeLa cells. The rat’s behavior, as well as the histopathological aspect of liver, kidney, lung, and spleen tissues at 48 h after intraperitoneal injection did not present any modifications. Thein vivoandin vitroassays suggested that the IO-NPs could be further used for developing newin vivomedical applications.

Efficient and Rapid Labeling of Transplanted Cell Populations with Superparamagnetic Iron Oxide Nanoparticles Using Cell Surface Chemical Biotinylation for in Vivo Monitoring by MRI

2010 ◽  
Vol 19 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Po-Wah So ◽  
Tammy Kalber ◽  
David Hunt ◽  
Michael Farquharson ◽  
Alia Al-Ebraheem ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Cell Populations ◽  
Oxide Nanoparticles ◽  
Signal Loss

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.

Polyethylenimine-coated superparamagnetic iron oxide nanoparticles impair in vitro and in vivo angiogenesis

2019 ◽  
Vol 21 ◽  
pp. 102063 ◽  
Author(s):  
Vladimir Mulens-Arias ◽  
José Manuel Rojas ◽  
Laura Sanz-Ortega ◽  
Yadileiny Portilla ◽  
Sonia Pérez-Yagüe ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Tumor Cell Targeting by Iron Oxide Nanoparticles Is Dominated by Different Factors In Vitro versus In Vivo

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0115636 ◽  
Author(s):  
Christian NDong ◽  
Jennifer A. Tate ◽  
Warren C. Kett ◽  
Jaya Batra ◽  
Eugene Demidenko ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Tumor Cell ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cell Targeting

scholarly journals Compact zwitterion-coated iron oxide nanoparticles for in vitro and in vivo imaging

2012 ◽  
Vol 5 (1) ◽  
pp. 108-114 ◽  
Author(s):  
He Wei ◽  
Oliver T. Bruns ◽  
Ou Chen ◽  
Moungi G. Bawendi
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
In Vivo Imaging ◽  
Oxide Nanoparticles

scholarly journals In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

2015 ◽  
Vol 16 (10) ◽  
pp. 24417-24450 ◽  
Author(s):  
Ujwal Patil ◽  
Shiva Adireddy ◽  
Ashvin Jaiswal ◽  
Sree Mandava ◽  
Benjamin Lee ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Toxicity Evaluation ◽  
In Vivo Toxicity

Casein-Coated Iron Oxide Nanoparticles for in vitro Hyperthermia for Cancer Therapy

SPIN ◽  
2019 ◽  
Vol 09 (02) ◽  
pp. 1940003 ◽  
Author(s):  
Milad Salimi Bani ◽  
Shadie Hatamie ◽  
Mohammad Haghpanahi ◽  
Hossein Bahreinizad ◽  
Mohammad Hossein Shahsavari Alavijeh ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Iron Oxide ◽  
Cancer Therapy ◽  
Iron Oxide Nanoparticles ◽  
Surrounding Medium ◽  
Treatment Method ◽  
Oxide Nanoparticles ◽  
One Pot ◽  
X Ray

Iron oxide nanoparticles (NPs) have been a very appealing choice in magnetic-mediated hyperthermia for cancer therapy. The responses of NPs to hyperthermia as a cancer treatment method are complex and variable. Herein, the heating properties of the casein-coated magnetic NPs (MNPs) under an alternating magnetic field were investigated. The casein-coated MNPs were synthesized via one-pot chemical method. The casein-coated MNPs were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), zeta potential, dynamic light scattering (DLS), and vibrating sample magnetometer (VSM) analysis. TEM images of casein-coated MNPs show that their shapes are spherical and their core sizes are between 20[Formula: see text]nm and 25[Formula: see text]nm. The FTIR and EDAX results confirmed the presence of casein on the surface of MNPs. The VSM shows the superparamagnetic nature of iron oxide and casein-coated iron oxide NPs with the magnetic saturation of 60[Formula: see text]emu/g and 44.86[Formula: see text]emu/g, respectively, at room temperature. Furthermore, hyperthermia tests for casein-coated MNPs with various concentrations and frequencies are performed. Hyperthermia results show that lower concentrations of casein-coated MNPs dispatch higher heating into their surrounding medium, whereas maximum specific absorption rate occurs at the concentration of 1[Formula: see text]mg/mL for the frequency of 150[Formula: see text]kHz. Findings of this study suggest that casein-coated MNPs have great potential as an anticancer agent in hyperthermia cancer therapy.

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