scholarly journals p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ssu-Ming Huang ◽  
Cheng-Fang Tsai ◽  
Dar-Ren Chen ◽  
Min-Ying Wang ◽  
Wei-Lan Yeh

Osthole has been reported to have antitumor activities via the induction of apoptosis and inhibition of cancer cell growth and metastasis. However, the detailed molecular mechanisms underlying the anticancer effects of osthole in human colon cancer remain unclear. In the present study, we have assessed osthole-induced cell death in two different human colon cancer cell lines, HCT116 and SW480. Our results also showed that osthole activated proapoptotic signaling pathways in human colon cancer cells. By using cell culture insert system, osthole reduced cell motility in both human colon cancer cell lines. This study also provides evidence supporting the potential of osthole in p53 activation. Expression of p53, an apoptotic protein, was remarkably upregulated in cells treated with osthole. Importantly, the levels of phosphorylation of p53 on Ser15 (p-p53) and acetylation of p53 on Lys379(acetyl-p53) were increased under osthole treatment. Our results also demonstrated that p53 was activated followed by generation of reactive oxygen species (ROS) and activation of c-Jun N-terminal kinase (JNK). Our study provides novel insights of p53-mediated responses under osthole treatment. Taken together, we concluded that osthole induces cancer cell death and inhibits migratory activity in a controlled manner and is a promising candidate for antitumor drug development.

2010 ◽  
Vol 62 (8) ◽  
pp. 1007-1016 ◽  
Author(s):  
Weimin Guo ◽  
Lin Nie ◽  
Dayong Wu ◽  
Mitchell L. Wise ◽  
F. William Collins ◽  
...  

2012 ◽  
Vol 12 (6) ◽  
pp. 8062-8070 ◽  
Author(s):  
FENG-QI FANG ◽  
HUI-SHU GUO ◽  
JIE ZHANG ◽  
LI-YING BAN ◽  
JI-WEI LIU ◽  
...  

2004 ◽  
Vol 211 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Satoshi Kokura ◽  
Norimasa Yoshida ◽  
Eiko Imamoto ◽  
Miho Ueda ◽  
Takeshi Ishikawa ◽  
...  

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