scholarly journals Electroanalytical Methodology for the Direct Determination of 2,4-Dichlorophenoxyacetic Acid in Soil Samples Using a Graphite-Polyurethane Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Fernanda Ramos de Andrade ◽  
Renata Alves de Toledo ◽  
Carlos Manoel Pedro Vaz
Keyword(s):  
Irreversible Process ◽  
Direct Determination ◽  
Parameters Optimization ◽  
Dichlorophenoxyacetic Acid ◽  
Reduction Peak ◽  
Undisturbed Soil ◽  
Polyurethane Composite ◽  
Experimental Parameters ◽  
2,4 Dichlorophenoxyacetic Acid

An electroanalytical methodology was developed for the direct determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) using a graphite-polyurethane composite electrode and square wave voltammetry (SWV). 2,4-D exhibited one reduction peak with characteristics of an irreversible process at −0.54 V (versus Ag/AgCl), which is controlled by the diffusion of the reagent on the electrode surface. After the experimental parameters optimization (pH 2.0,f=50 s−1,a=0.50 V, andΔEi=0.03 V), analytical curves were constructed in the range of 0.66 mg L−1to 2.62  mg L−1. Detection (LD) and quantification (LQ) limits were 17.6 μg L−1and 58.6 μg L−1, respectively. The methodology was successfully applied to measure the percolation of the herbicide 2,4-D in undisturbed soil columns of different granulometric compositions.

Amplifying Bienzyme Cycle-linked Immunoassays for Determination of 2,4-Dichlorophenoxyacetic Acid

1996 ◽  
Vol 799 (1 Enzyme Engine) ◽  
pp. 519-524 ◽  
Author(s):  
FRANK F. BIER ◽  
EVA EHRENTREICH-FÕRSTER ◽  
FRIEDER W. SCHELLER
Keyword(s):  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

scholarly journals Optimization of the Conditions for Determining 2,4-D and Deltamethrin by HPLC

2020 ◽  
Vol 20 (4) ◽  
pp. 372-377
Author(s):  
Elena V. Melikhova ◽  
◽  
Olga V. Farafonova ◽  
Keyword(s):  
River Water ◽  
Chromatographic Determination ◽  
Capillary Columns ◽  
Dichlorophenoxyacetic Acid ◽  
Separate Determination ◽  
Isocratic Elution ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Gradient Mode

The paper presents the results of optimizing the conditions for the chromatographic determination of 2,4-dichlorophenoxyacetic acid and deltamethrin for two capillary columns (Supelcosil LC-18, Kromasil C-18). The modes of individual chromatography of pesticides were selected. The use of the isocratic elution mode in the joint presence of 2,4-dichlorophenoxyacetic acid and deltamethrin was shown and experimentally proved to be invalid. Three options were proposed for the conditions of the gradient mode of the separate detection of analytes in a mixture. The developed technique for the chromatographic separate determination of 2,4-D and deltamethrin with their joint presence was tested via analysis of river water and potato samples.

scholarly journals Characterization of Hapten-Protein Conjugates: Antibody Generation and Immunoassay Development for Chlorophenoxyacetic Acid Pesticides

2009 ◽  
Vol 92 (6) ◽  
pp. 1773-1779 ◽  
Author(s):  
Robin C Boro ◽  
K Vikas Singh ◽  
C Raman Suri
Keyword(s):  
Inhibitory Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Protein Conjugation ◽  
Protein Conjugates ◽  
Highly Sensitive ◽  
Ic50 Values ◽  
Chlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Abstract The generation of specific and sensitive antibodies against small molecules is greatly dependent upon the characteristics of the hapten-protein conjugates. In this study, we report a new fluorescence-based method for the characterization of hapten-protein conjugates. The method is based on an effect promoted by hapten-protein conjugation density upon the fluorescence intensity of the intrinsic tryptophan chromophore molecules of the protein. The proposed methodology is applied to quantify the hapten-protein conjugation density for two different chlorophenoxyacetic acid pesticides, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenoxybutyric acid (2,4-DB), coupled to carrier protein. Highly sensitive anti-2,4-D and anti-2,4-DB antibodies were obtained using these well-characterized hapten-protein conjugates. The generated antibodies were used in an immunoassay format demonstrating inhibitory concentration (IC50) values equal to 30 and 7 ng/mL for 2,4-D and 2,4-DB, respectively. Linearity was observed in the concentration range between 0.1500 ng/mL with LODs around 4 and 3 ng/mL for 2,4-D and 2,4-DB, respectively, in standard water samples. The proposed method was successfully applied for the determination of the extent of hapten-protein conjugation to produce specific antibodies for immunoassay development against pesticides.

Determination of the stoichiometry of 2,4-dichlorophenoxyacetic acid-β-cyclodextrin complexes in solution and in solid state

1998 ◽  
Vol 321 (1-2) ◽  
pp. 53-58 ◽  
Author(s):  
J.M. Ginés ◽  
M.J. Arias ◽  
J.I. Pérez-Martı́nez ◽  
J.R. Moyano ◽  
E. Morillo ◽  
...  
Keyword(s):  
Solid State ◽  
Dichlorophenoxyacetic Acid ◽  
Cyclodextrin Complexes ◽  
2,4 Dichlorophenoxyacetic Acid
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