Comparative Assessment of Factors Involved in Acetoin Synthesis by Bacillus subtilis 168

Acetoin is widely used as flavor agent and serves as a precursor for chemical synthesis. Here we focused on identifying the best physiological conditions (initial substrate concentrations, pH, temperature, and agitation) for enhanced acetoin accumulation by Bacillus subtilis 168. The optimal physiological conditions support maximum acetoin accumulation by minimizing byproduct (acetate and butanediol) synthesis and a maximum of 75% enhancement in acetoin yield could be achieved. Additionally, the effect of change in ALS (acetolactate synthase) and ALDC (acetolactate decarboxylase) activities was evaluated on acetoin accumulation. Increasing ALS and ALDC enzyme activities led to efficient utilization of pyruvate towards acetoin accumulation and about 80% enhancement in acetoin accumulation was observed.

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Faculty Opinions recommendation of Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1029612.347484 ◽

2005 ◽

Author(s):

John Glass

Keyword(s):

Bacillus Subtilis ◽

Synechocystis Pcc6803 ◽

Bacillus Subtilis 168

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Faculty Opinions recommendation of The Min system and nucleoid occlusion are not required for identifying the division site in Bacillus subtilis but ensure its efficient utilization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.14263040.15775167 ◽

2012 ◽

Author(s):

Petra Levin

Keyword(s):

Bacillus Subtilis ◽

Efficient Utilization ◽

Division Site

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Phosphorus Release Kinetics in Biofilm Reactors

Water Science & Technology ◽

10.2166/wst.1992.0436 ◽

1992 ◽

Vol 26 (3-4) ◽

pp. 567-576 ◽

Cited By ~ 2

Author(s):

F. A. Ruiz-Treviño ◽

S. González-Martínez ◽

C. Doria-Serrano ◽

M. Hernández-Esparza

Keyword(s):

Phosphorus Removal ◽

Release Kinetics ◽

Phosphorus Release ◽

Organic Substrate ◽

Biofilm Reactor ◽

Substrate Uptake ◽

Biofilm Reactors ◽

Initial Substrate ◽

Linear Behaviour ◽

Substrate Concentrations

This paper presents the kinetic analysis, using Generalized Power-Law equations to describe the results of an experimental investigation conducted on a batch submerged biofilm reactor for phosphorus removal under an anaerobic/aerobic cycle. The observed rates and amounts of phosphorus release and organic substrate uptake in the anaerobic phase leads to a kinetic model in which these two variables are dependent on each other with a non-linear behaviour and reach equilibrium values in both cases, at different times and are function of rate constants ratio. The model has a good fit with experimental data except for C uptake at anaerobic contact times longer than four hours, where other kinetics are implied. Kinetic parameters were obtained with different initial substrate concentrations, anaerobic contact cycles, and type of substrates.

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