Protective Effects ofElaeagnus angustifoliaLeaf Extract against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart

The purpose of this study is to clarify the cardioprotective property of the aqueous extract ofElaeagnus angustifoliaL. leaf (EA) against myocardial ischemia/reperfusion injury in isolated rat heart. The myocardial ischemia/reperfusion (I/R) injury model of isolated rat heart was set up by the use of improved Langendorff retrograde perfusion technology. Compared with the ischemia/reperfusion (I/R) group, the aqueous extract ofElaeagnus angustifoliaL. leaf (0.5 mg/mL, 1.0 mg/mL) pretreatment markedly improved the coronary flow (CF) and raised left ventricular developed pressure (LVDP) and maximum rise/down velocity (±dp/dtmax). The infarct size of the EA-treated hearts was smaller than that of I/R group. After treatment with EA, the superoxide dismutase (SOD) activity increased; malondialdehyde (MDA) and protein carbonyl content reduced more obviously (P<0.01) than that of I/R injury myocardial tissue.Conclusion. Results from the present study showed that the aqueous extract ofElaeagnus angustifoliaL. leaf has obvious protective effects on myocardial I/R injury, which may be related to the improvement of myocardial oxidative stress states.

Download Full-text

Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.06.068 ◽

2017 ◽

Vol 93 ◽

pp. 626-635 ◽

Cited By ~ 10

Author(s):

Zhibin Wang ◽

Gaosong Wu ◽

Hua Liu ◽

Na Xing ◽

Yanping Sun ◽

...

Keyword(s):

Myocardial Ischemia ◽

Reperfusion Injury ◽

Rat Heart ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Isolated Rat Heart ◽

Cardioprotective Effect ◽

Myocardial Ischemia Reperfusion Injury ◽

Myocardial Ischemia Reperfusion ◽

Isolated Rat

Download Full-text

Cardioprotective Effects of Astragalin against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/8194690 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 21

Author(s):

Daoxu Qu ◽

Jichun Han ◽

Huanhuan Ren ◽

Wenxiao Yang ◽

Xinjie Zhang ◽

...

Keyword(s):

Myocardial Ischemia ◽

Rat Heart ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Isolated Rat Heart ◽

Sprague Dawley ◽

Sod Activity ◽

Cardioprotective Effects ◽

Myocardial Ischemia Reperfusion ◽

Isolated Rat

This study aims to evaluate the cardioprotective effects of astragalin against myocardial ischemia/reperfusion (I/R) injury in isolated rat heart. The cardioprotective effects of astragalin on myocardial I/R injury were investigated on Langendorff apparatus. Adult male Sprague-Dawley rats were randomly divided into five groups. The results showed that astragalin pretreatment improved myocardial function. Compared with I/R group, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in coronary flow decreased in astragalin pretreatment groups, whereas superoxide dismutase (SOD) activity and glutathione/glutathione disulfide (GSH/GSSG) ratio significantly increased. The levels of malondialdehyde (MDA), intracellular reactive oxygen species (ROS), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6) decreased in astragalin-treated groups. The infarct size (IS) and apoptosis rate in hearts from astragalin-treated groups were lower than those in hearts from the I/R group. Western blot analysis also revealed that astragalin preconditioning significantly reduced Bax level, whereas Bcl-2 was increased in the myocardium. Therefore, astragalin exhibited cardioprotective effects via its antioxidative, antiapoptotic, and anti-inflammatory activities.

Download Full-text