Photobiomodulation for Cobalt Chloride-Induced Hypoxic Damage of RF/6A Cells by 670 nm Light-Emitting Diode Irradiation

Objective. The goal of this study was to investigate the therapeutic efficacy of 670 nm light-emitting diode (LED) irradiation on the diabetic retinopathy (DR) using hypoxic rhesus monkey choroid-retinal (RF/6A) cells as the model system.Background Data. Treatment with light in the spectrum from red to near-infrared region has beneficial effect on tissue injury and 670 nm LED is currently under clinical investigation for retinoprotective therapy.Methods. Studies were conducted in the cultured cells under hypoxia treated by cobalt chloride (CoCl2). After irradiation by 670 nm LED with different power densities, cell viability, cytochrome C oxidase activity, and ATP concentration were measured.Results. The irradiation of 670 nm LED significantly improved cell viability, cytochrome C oxidase activity, and ATP concentration in the hypoxia RF/6A cells.Conclusion. 670 nm LED irradiation could recover the hypoxia damage caused by CoCl2. Photobiomodulation of 670 nm LED plays a potential role for the treatment of diabetic retinopathy.

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Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation

Journal of Biophotonics ◽

10.1002/jbio.201400087 ◽

2014 ◽

Vol 8 (9) ◽

pp. 740-754 ◽

Cited By ~ 34

Author(s):

Cleber Ferraresi ◽

Nivaldo Antonio Parizotto ◽

Marcelo Victor Pires de Sousa ◽

Beatriz Kaippert ◽

Ying-Ying Huang ◽

...

Keyword(s):

Cell Proliferation ◽

Cytochrome C ◽

Cytochrome C Oxidase ◽

Oxidase Activity ◽

Light Emitting Diode ◽

Muscle Performance ◽

Light Emitting

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Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation [J. Biophotonics 8, No. 9, 740-754 (2015)]

Journal of Biophotonics ◽

10.1002/jbio.201680087 ◽

2016 ◽

Vol 9 (9) ◽

pp. 976-976 ◽

Cited By ~ 3

Author(s):

Cleber Ferraresi ◽

Nivaldo Antonio Parizotto ◽

Marcelo Victor Pires de Sousa ◽

Beatriz Kaippert ◽

Ying-Ying Huang ◽

...

Keyword(s):

Cell Proliferation ◽

Cytochrome C ◽

Cytochrome C Oxidase ◽

Oxidase Activity ◽

Light Emitting Diode ◽

Muscle Performance ◽

Light Emitting

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Photoactivation of cytochrome с oxidase activity in liver mitochondria of Japanese quail by therapeutic doses of blue and red LED irradiation

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2018.03.25-30 ◽

2018 ◽

pp. 25-30

Author(s):

А.Е. Донцов ◽

Н.Б. Сережникова ◽

Л.С. Погодина ◽

Т.С. Гурьева ◽

П.П. Зак

Keyword(s):

Cytochrome C ◽

Cytochrome C Oxidase ◽

Blue Light ◽

Japanese Quail ◽

Oxidase Activity ◽

Red Light ◽

Liver Mitochondria ◽

Coturnix Japonica ◽

Red Led ◽

Led Irradiation

Цель исследования - выяснение влияния синего (450 нм) и красного (630 нм) светодиодного облучения различной энергетической экспозиции на активность митохондриальной цитохром с-оксидазы. Методики. Объектом исследования служили митохондрии, изолированные из печени японского перепела Coturnix japonica . Цитохром с-оксидазную активность измеряли по скорости окисления восстановленного тетраметил-р-фенилендиамина в присутствии ротенона. Результаты. Показано, что облучение митохондрий синим светом с энергетическими экспозициями, не превышающими 4 Дж/см вызывает примерно 5-15-процентную стимуляцию активности цитохром с-оксидазы, а дозы больше 5 Дж/см приводят к ингибированию активности фермента. Облучение митохондрий красным светом также оказывает стимулирующее действие (10-20%) на цитохром с-оксидазную активность по сравнению с необлученными образцами. Заключение. Предполагается, что низкодозовое облучение синим светом может иметь сходное с красным светом терапевтическое действие при фотобиомодуляции. The aim was to study the effect of blue (450 nm) and red (630 nm) LED irradiation with different energy exposures on activity of mitochondrial cytochrome c oxidase. Methods. The study was performed on mitochondria isolated from the liver of Japanese quail Coturnix japonica . Cytochrome c oxidase activity was measured by the rate of oxidation of reduced tetramethyl-p-phenylenediamine in the presence of rotenone. Results. Irradiation of mitochondria with blue light at doses not exceeding 4 J/cm caused approximately 5-15% stimulation of cytochrome c oxidase activity while doses higher than 5 J/cm led to inhibition of this enzyme. Irradiation of mitochondria with red light also exerted a slight stimulating effect (10-20%) on cytochrome c oxidase activity compared to unirradiated samples. Conclusion. The study suggested that low-dose irradiation with blue light may produce a therapeutic effect similar to red light in photobiomodulation.

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