Identification and Quality Assessment of Chrysanthemum Buds by CE Fingerprinting

A simple and efficient fingerprinting method for chrysanthemum buds was developed with the aim of establishing a quality control protocol based on biochemical makeup. Chrysanthemum bud samples were successively extracted by water and alcohol. The fingerprints of the chrysanthemum buds samples were obtained using capillary electrophoresis and electrochemical detection (CE-ED) employing copper and carbon working electrodes to capture all of the chemical information. 10 batches of chrysanthemum buds were collected from different regions and various factories to establish the baseline fingerprint. The experimental data of 10 batches electropherogram buds by CE were analyzed by correlation coefficient and the included angle cosine methods. A standard chrysanthemum bud fingerprint including 24 common peaks was established, 12 from each electrode, which was successfully applied to identify and distinguish between chrysanthemum buds from 2 other chrysanthemum species. These results demonstrate that fingerprint analysis can be used as an important criterion for chrysanthemum buds quality control.

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Quality Assessment and Origin Identification on Four Species of Curcumae Radix by HPLC Assay and Fingerprint Analysis

10.21203/rs.3.rs-838863/v1 ◽

2021 ◽

Author(s):

Yu-Ling Ho ◽

Chi-Ren Liao ◽

Chun-Pin Kao ◽

Kun-Chang Wu ◽

Shang-Chih Lai ◽

...

Keyword(s):

Quality Control ◽

Chinese Medicine ◽

Quality Assessment ◽

The Other ◽

Hplc Assay ◽

Fingerprint Analysis ◽

Relative Peak Area ◽

Simple Method ◽

Definite Difference ◽

Origin Identification

Abstract BackgroundCurcumae Radix is a multi-source Chinese medicine called Yujin. It is derived from the root tubers of four Curcuma species including Curcuma wenyujin , C. kwangsiensis , C. phaeocaulis and C. longa. The identification of the root tubers of the four Curcuma species often caused confusions. MethodsIn this study, we developed HPLC/PDA methods to differentiate these four species of Curcumae Radix by HPLC assay and fingerprint analysis. The methods developed were also validated with their precision, repeatability and accuracy. ResultsCurdione could only be detected in C. wenyujin . For C. kwangsiensis , C. phaeocaulis , if the relative peak area value of peak 1 and germacrone peak of the samples was more than 1.024, it could be identified as C. phaeocaulis of Curcumae Radix. Curcumin, desmethoxycurcumin and bisdesmethoxycurcumin could only be detected in C. longa . We also developed a simple method to determine curcumin, desmethoxycurcumin and bisdesmethoxycurcumin in C. lon ga and germacrone in the other three species of Curcumae Radix. ConclusionsBecause of the definite difference of main ingredients between curcumin-free group (C. wenyujin , C. kwangsiensis and C. phaeocaulis ) and curcumin containing C. longa from this study, four Curcuma species of Curcumae Radix in the pharmacopeia should be separated into two monographs, with curcumin-free and germacrone containing species as one and curcumin containing C. longa as the second monograph. The HPLC/PDA methods of HPLC assay and fingerprint analysis developed in this study could be used for the quality control of these four species of Curcumae Radix. Keywords: Curcumae Radix, HPLC, assay, fingerprint, curcumin, desmethoxycurcumin, bisdesmethoxycurcumin, germacrone, curdione

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Quality-control results for ground-water and surface-water data, Sacramento River Basin, California, National Water-Quality Assessment, 1996-1998

10.3133/wri024201 ◽

2003 ◽

Keyword(s):

Water Quality ◽

Quality Control ◽

Surface Water ◽

Quality Assessment ◽

Ground Water ◽

River Basin ◽

Water Quality Assessment ◽

Sacramento River ◽

National Water

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Chemical Fingerprint Analysis and Simultaneous Determination of Nucleosides and Amino Acids in Kang Fu Xin Liquid by High Performance Liquid Chromatography with Diode Array Detector

Current Pharmaceutical Analysis ◽

10.2174/1573412915666190328215231 ◽

2020 ◽

Vol 16 (7) ◽

pp. 831-843

Author(s):

Yuwen Wang ◽

Shuping Li ◽

Liuhong Zhang ◽

Shenglan Qi ◽

Huida Guan ◽

...

Keyword(s):

Amino Acids ◽

Quality Control ◽

Uric Acid ◽

Control Method ◽

Principal Component ◽

Gradient Elution ◽

Hplc Method ◽

Array Detector ◽

Fingerprint Analysis ◽

Wavelength Switching

Background and Objective: Kang Fu Xin liquid (KFX) is an official preparation made from the ethanol extract product from P. Americana. The present quality control method cannot control the quality of the preparation well. The aim of the present study is to establish a convenient HPLC method for multicomponents determination combined with fingerprint analysis for quality control of KFX. Methods: An HPLC-DAD method with gradient elution and detective wavelength switching program was developed to establish HPLC fingerprints of KFX, and 38 batches of KFX were compared and evaluated by similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA). Meanwhile, six nucleosides and three amino acids, including uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan in KFX were determined based on the HPLC fingerprints. Results: An HPLC method assisted with gradient elution and wavelength switching program was established and validated for multicomponents determination combined with fingerprint analysis of KFX. The results demonstrated that the similarity values of the KFX samples were more than 0.845. PCA indicated that peaks 4 (hypoxanthine), 7 (xanthine), 9 (tyrosine), 11, 13 and 17 might be the characteristic contributed components. The nine constituents in KFX, uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan, showed good regression (R2 > 0.9997) within test ranges and the recoveries of the method for all analytes were in the range from 96.74 to 104.24%. The limits of detections and quantifications for nine constituents in DAD were less than 0.22 and 0.43 μg•mL-1, respectively. Conclusion: The qualitative analysis of chemical fingerprints and the quantitative analysis of multiple indicators provide a powerful and rational way to control the KFX quality for pharmaceutical companies.

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