scholarly journals Effect of Naphthalene Acetic Acid on the Adventitious Rooting in Shoot Cuttings ofAndrographis paniculata(Burm.f.) Wall. ex Nees: An Important Therapeutical Herb

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Sanower Hossain ◽  
Zannat Urbi
Keyword(s):  
Adventitious Rooting ◽  
Peat Moss ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Medical Systems ◽  
Once Daily ◽  
Apical Shoot ◽  
Commercial Scale ◽  
Rooting Response

Andrographis paniculatais one of the most important therapeutical herbs, widely used in traditional medical systems for the treatment of diverse diseases for thousands of years. This study was carried out to assess the effect of 1-naphthaleneacetic acid (NAA) on adventitious rooting inA. paniculatashoot cuttings. The cuttings were treated with six concentrations of NAA (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mM) by applying soaking method and cuttings without hormone (soaking in distilled water) were considered as control. The cuttings were then inoculated into peat moss in the planting tray and incubated under complete shade for root induction. Water was sprayed on peat moss once daily to moisten it. The results showed that different concentrations of NAA significantly (P≤0.05) affected the rooting characteristics ofA. paniculataand 2.5 mM of NAA was found to be more effective to induce rooting in young apical shoot (YAS) cuttings compared to other concentrations and old apical shoot (OAS). This study also postulates that adventitious rooting response depends on the juvenility of plant material and concentration of growth regulator. This report describes a technique for adventitious rooting inA.paniculata, which could be feasible to use for commercial scale propagation of this plant.

scholarly journals Adventitious Rooting of Lavandula x intermedia Cuttings

2021 ◽  
Vol 39 (4) ◽  
pp. 150-159
Author(s):  
Derek Reed ◽  
Thayne Montague ◽  
Catherine Simpson
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Adventitious Rooting ◽  
Peat Moss ◽  
Media Selection ◽  
Naphthaleneacetic Acid ◽  
Effects Of Media ◽  
Root Quality

Abstract Due to challenges associated with lavender propagation, Texas hybrid lavender (lavandin) growers require revised propagation information. Therefore, experiments investigated effects of media and plant growth regulators (PGR) on rooting of three different lavandin (Lavandula x intermedia) cultivars. PGRs applied to cuttings were naphthaleneacetic acid (NAA), or indole-3-butyric acid (IBA) at three concentrations. In addition, four rooting media were examined. Cuttings exposed to a peat moss/sand medium had the greatest mean overall rooting percentage, number of roots, and regardless of cultivar, cuttings exposed to the peat moss/sand medium had the longest roots. In general, mean rooting data were also greatest for cuttings dipped in NAA at 0.5 and 1.0 g·L−1 (500 and 1000 ppm, respectively). Overall, across all media and PGR treatments ‘Grosso' cuttings tended to have the greatest rooting percentage and longest roots. In addition, compared to the no PGR treatment, ‘Provence' and ‘Hidcote Giant' cuttings exposed to the peat moss/sand medium and greater PGR concentrations produced cuttings with an increased mean rooting percentage and longer roots. When rooting lavandin cultivars, results indicate correct media selection, PGR formulation, and PGR concentration determine propagation success. Index words: lavandin, Lavandula x intermedia Emeric ex Loisel, peat moss, perlite, rooting percentage, root quality, sand. Chemicals used in this study: indole-3-butyric acid (IBA; 0.5, 1.0, and 1.5 g.L−1); naphthaleneacetic acid (NAA; 0.5, 1.0, and 1.5 g.L−1). Species used in this study: ‘Grosso', ‘Provence', and ‘Hidcote Giant' lavandin (Lavandula x intermedia Emeric ex Loisel.).

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Development of a robust method for propagation of Leptospermum scoparium J.R.Forst. & G.Forst. from semi-hardwood cuttings

2020 ◽  
Author(s):  
Tate Jason James Hancox ◽  
Rachel A Burton ◽  
Kate Louise Delaporte
Keyword(s):  
Clonal Propagation ◽  
Natural Populations ◽  
Naphthalene Acetic Acid ◽  
Maturity Stage ◽  
Root Induction ◽  
Indole Butyric Acid ◽  
Transplant Survival ◽  
Detailed Method ◽  
Significant Difference ◽  
Leptospermum Scoparium

Abstract BackgroundLeptospermum scoparium J.R.Forst. & G.Forst. is highly valued for the production of antimicrobial honey. Both researchers and the Australian honey industry are interested in the clonal propagation of these plants to be used for experiments and plantation establishment. However, little information is available on clonal propagation by cuttings. Here we sought to develop a detailed method for L. scoparium propagation by semi-hardwood cuttings by testing the influence of genotype and auxin (indole butyric acid (IBA) and IBA + naphthalene acetic acid (NAA)) concentration on plants originating from four natural populations in Victoria, Australia. ResultsThere was no significant difference in root induction, which ranged from 50.9 – 68.0%, in the four clonal source plants selected. The application of 3 g/L IBA increased root induction from 46.6% of the control cuttings to 77.6% of treated cuttings and stimulated longer roots. The application of 8 g/L IBA increased leaf fall, the presence of necrosis, from 22.5% on the controls to 82.8% on dipped cuttings, and cutting death.Although there was no significant difference in root induction, cuttings that were ‘watered-in’ after planting dropped significantly fewer leaves and had significantly lower rates of necrosis than cuttings watered in after 2-3 days. It was also found that the maturity stage of semi-hardwood cuttings did not significantly affect any of the variables measured. Transplant survival ranged from 78.1 – 89.0% for cuttings treated with auxin and 77.3 to 92.2% for the four clonal source plants. ConclusionThis study found that the clonal source plants selected did not affect the success of propagation. Overall for maximum success, semi-hardwood cuttings can be collected from the current or previous seasons growth, be treated with 3 g/L IBA gel, ‘watered in’ and placed into a propagation tent for approximately six weeks. Cuttings that form roots will transplant easily with high transplant survival.

Effect of polar auxin transport and gibberellins on xylem formation in pine cuttings under adventitious rooting conditions

2020 ◽  
Vol 67 (1-2) ◽  
pp. 27-39 ◽  
Author(s):  
Alberto Pizarro ◽  
Carmen Díaz-Sala
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Auxin Transport ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
Polar Auxin Transport ◽  
Root Induction ◽  
Xylem Differentiation ◽  
Xylem Formation ◽  
Hypocotyl Cuttings

Maturation-related decline of adventitious root formation is one of the major factors affecting adventitious rooting in forest tree species. We demonstrate that inhibition of polar auxin transport promoted cambium and xylem differentiation in rooting-competent hypocotyl cuttings from Pinus radiata under conditions of adventitious root formation. Treatments with bioactive gibberellins inhibited rooting while at the same time inducing both the differentiation of a continuous ring of cambium and xylem formation. Treatments with inhibitors of gibberellin biosynthesis did not affect the rooting response. The results demonstrate that xylem parenchyma and procambial cells at the xylem poles of rooting-competent hypocotyl cuttings after excision and under conditions of adventitious root induction become adventitious root meristems or xylem, depending on the directional auxin flow. Gibberellin may interact with this pathway, inducing xylem differentiation and inhibiting rooting. We conclude that modifications of auxin flow at the rooting sites, and the priming of cambial cells to differentiate into xylem during tree ageing, may be associated with the maturation-related decline of adventitious root formation.

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals Establishment of a Rapid and Efficient Micropropagation System for Succulent Plant Haworthia turgida Haw.

HortScience ◽  
2017 ◽  
Vol 52 (9) ◽  
pp. 1278-1282 ◽  
Author(s):  
Boling Liu ◽  
Hongzhou Fang ◽  
Chaorong Meng ◽  
Ming Chen ◽  
Qingdong Chai ◽  
...  
Keyword(s):  
Callus Induction ◽  
Adventitious Shoots ◽  
Germplasm Conservation ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Succulent Plant

In the present study, the effect of plant growth regulators (PGRs) on callus regeneration, adventitious shoot differentiation, and root formation of Haworthia turgida Haw. was investigated. The greatest callus induction percentage (95.6%) was achieved with leaf explants inoculated on Murashige and Skoog (MS) medium with 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 1-naphthaleneacetic acid (NAA), and this callus induction medium supplemented with 2.5 mg·L−1 thidiazuron (TDZ) was optimal for callus proliferation. The maximum number of shoots (25.7) was obtained when the callus was cultured on MS medium supplemented with 1.0 mg·L−1 BA and 0.2 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest number of roots per shoot (6.2) and highest rooting frequency (82.0%) were obtained when adventitious shoots were inoculated on MS medium with 0.05 mg·L−1 NAA. Regenerated plantlets were transferred to a mixture of vermiculite and soil and acclimated in a greenhouse. The survival rate of the transplanted plantlets was about 91.6%. The rate of ex vitro rooting was 83.3%, indicating that this technique is effective for root induction in H. turgida. This study has established a rapid and efficient micropropagation system that can be beneficial for commercial cultivation and germplasm conservation of H. turgida.

scholarly journals Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Saccharum Officinarum ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

scholarly journals Micropropagation and Quantification of Bioactive Compounds in Mertensia maritima (L.) Gray

2019 ◽  
Vol 20 (9) ◽  
pp. 2141 ◽  
Author(s):  
Han Yong Park ◽  
Doo Hwan Kim ◽  
Ramesh Kumar Saini ◽  
Judy Gopal ◽  
Young-Soo Keum ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Large Scale ◽  
Shoot Tip ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Leaf Tissues

The goal of this study was to establish an efficient protocol for the large-scale propagation of Mertensia maritima (L.) Gray, and evaluate the carotenoid, fatty acid, and tocopherol contents in the leaves of in vitro regenerated shoots. Surface-disinfected node and shoot tip explants were placed on semisolid Murashige and Skoog (MS) medium with 0–16 µM N6-benzyladenine (BA), kinetin, (KN), and thidiazuron (TDZ) alone, or in combination with, 1 or 2 µM α-naphthaleneacetic acid (NAA). Of the three different cytokinins employed, TDZ elicited the best results for axillary shoot proliferation. A maximum frequency of shoot initiation above 84%, with a mean of 8.9 and 4.8 shoots per node and shoot tip, respectively, was achieved on the culture medium supplemented with 4 µM TDZ. A combination of TDZ + NAA significantly increased the percentage of multiple shoot formation and number of shoots per explant. The best shoot induction response occurred on MS medium with 4 µM TDZ and 1 µM NAA. On this medium, the node (93.8%) and shoot tip (95.9%) explants produced an average of 17.7 and 8.6 shoots, respectively. The highest root induction frequency (97.4%) and number of roots per shoot (25.4), as well as the greatest root length (4.2 cm), were obtained on half-strength MS medium supplemented with 4 µM indole-3-butyric acid (IBA). The presence of six carotenoids and α-tocopherol in the leaf tissues of M. maritima was confirmed by HPLC. Gas chromatography-mass spectrometry analysis confirmed the presence of 10 fatty acids, including γ-linolenic acid and stearidonic acid in the leaf tissues of M. maritima. All-E-lutein (18.49 μg g−1 fresh weight, FW), α-tocopherol (3.82 μg g−1 FW) and α-linolenic acid (30.37%) were found to be the significant compounds in M. maritima. For the first time, a successful protocol has been established for the mass propagation of M. maritima with promising prospects for harnessing its bioactive reserves.

An improved culture medium for growing the orchid Cypripedium reginae axenically

1982 ◽  
Vol 60 (12) ◽  
pp. 2547-2555 ◽  
Author(s):  
Gaëtan Harvais
Keyword(s):  
Acetic Acid ◽  
Membrane Filtration ◽  
Ammonium Citrate ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Growth And Survival ◽  
Murashige Skoog ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Germination And Growth

A new medium for growing Cypripedium reginae Walt. axenically from seed was designed. Liquid culture proved unsuitable, hence a 1% agar medium supplemented with 5% potato extract was used to investigate optimal mineral element, vitamin, amino acid, sugar, and growth regulator supplements for germination, and subsequent growth. A modified Pfeffer solution with 1400 mg/L NH4NO3 + 19 mg/L ammonium citrate + 2% dextrose + 10 mg/L niacin + 5 mg/L calcium pantothenate + 5 mg/L thiamine HCl + 1 mg/L kinetin + 0.1 mg/L α-naphthaleneacetic acid gave best germination and growth to 2 years with little or no phenolic production. Gamborg's B5 medium and Murashige–Skoog (MS) medium were less than optimal when tested against the above medium. Growth regulators were more active when sterilized by membrane filtration instead of autoclaving. Of the three aminopurines tested, kinetin, benzylaminopurine (BAP), and 6(γ,γ-dimethylallylamino) purine (γγ), the order of activity was initially γγ → BAP → kinetin, but kinetin produced better greening of protocorms and plantlets, and eventually greater survival. Hence, it was chosen for further study. The auxins indole-3-acetic acid (IAA), naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) were also tested alone and in combination with the aminopurines. They did not stimulate germination, but improved growth and survival when combined with aminopurines. The most active of the auxins were NAA → IAA → IBA → 2,4-D. A kinetin:NAA ratio of 10:1 was very satisfactory.

scholarly journals Optimization of the micropropagation of elite adult trees of Sequoia sempervirens: forest species of interest in the Basque Country, Spain

Bionatura ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 1511-1519
Author(s):  
Alejandra Rojas Vargas ◽  
A Castander-Olarieta ◽  
P Moncaleán ◽  
I.A Montalbán
Keyword(s):  
Climate Change ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Pinus Radiata ◽  
Basque Country ◽  
Sequoia Sempervirens ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Forest Species ◽  
Forestry Sector

Forest trees are renewable sources of timber and other valuable non-timber products. Nowadays, the increase in population and demand for forestry products results in overexploitation of forestry. Therefore, there is an urgent need to produce elite plants with higher productivity under stress derived from climate change to have available to afforestation. For this reason, propagation methods should be improved to be more efficient in terms of quality and productivity. The main species planted in the Basque Country is Pinus radiata; during the last three years, Pinus radiata plantations have suffered a fungus attack affecting mainly needles until the tree's death. This crisis is caused by the combined action of two fungi of the genus Dothistroma and Lecanosticta acicola, whose expansion seems to have been enhanced by weather-related factors, such as humid and hot summers. Although we have evidence of this disease's presence in our mountains since 1942, the disease has had a speedy expansion with an aggressive effect for reasons that are not scientifically known today. For the above, Basque Country forestry sector is looking for alternative species to be used in its plantations. Part of the forestry sector considers that Sequoia sempervirens could be a good choice for plantations. Besides, its high-quality wood and its tolerance to the attack of several pathogens and other diseases derived from climate change are characteristics that could confer some advantage over other forest species. The main goal of this study was to optimize the micropropagation of adult elite trees of Sequoia sempervirens. The effect of 6-benzylaminopurine, meta-Topolin and Kinetin, and 4 types of explant in the multiplication stage were analyzed to carry out this objective. Furthermore, the effect of two types of auxins: 1-naphthalene acetic acid, indole-3-butyric acid, and a mixture of both, were evaluated on the induction of roots and their subsequent effect on the acclimatization process. The best multiplication index was obtained when 4.4 µM 6-benzylaminopurine and apical explants longer than 1.5 cm of length were used. The root induction percentage was 75% in the most responsive genotype analyzed when 4.4 µM 6-benzylaminopurine was used on the induction stage, and 50 µM of 1-naphthalene acetic acid was used for rooting. Finally, after 3 months in the greenhouse, the explants cultured with Kinetin and rooted in a culture medium with indole-3-butyric acid showed the highest acclimatization success (94%).

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