scholarly journals Comparison of Signaling Pathways Gene Expression in CD34−Umbilical Cord Blood and Bone Marrow Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Rafał Stojko ◽  
Monika Bojdys-Szyndlar ◽  
Agnieszka Drosdzol-Cop ◽  
Andrzej Madej ◽  
Krzysztof Wilk
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cord Blood ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Biological Processes ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

The aim of the study was to compare the biological activity of the total pool of genes in CD34−umbilical cord blood and bone marrow stem cells and to search for the differences in signaling pathway gene expression responsible for the biological processes. The introductory analysis revealed a big similarity of gene expression among stem cells. When analyzing GO terms for biological processes, we observed an increased activity of JAK-STAT signaling pathway, calcium-mediated, cytokine-mediated, integrin-mediated signaling pathway, and MAPK in a cluster of upregulating genes in CD34−umbilical cord blood stem cells. At the same time, we observed a decreased activity of BMP signaling pathways, TGF-beta pathway, and VEGF receptor signaling pathway in a cluster of downregulating genes in CD34−umbilical cord blood stem cells. In accordance with KEGG classification, the cytokine-cytokine receptor interaction, toll-like receptor signaling pathway, and JAK-STAT signaling pathway are overrepresented in CD34−umbilical cord blood stem cells. A similar gene expression in both CD34−UCB and BM stem cells was characteristic for such biological processes as cell division, cell cycle gene expression, mitosis, telomere maintenance with telomerase, RNA and DNA treatment processes during cell division, and similar genes activity of Notch and Wnt signaling pathways.

scholarly journals From cord to caudate: characterizing umbilical cord blood stem cells and their paracrine interactions with the injured brain

2017 ◽  
Vol 83 (1-2) ◽  
pp. 205-213 ◽  
Author(s):  
Priya F Maillacheruvu ◽  
Lauren M Engel ◽  
Isaiah T Crum ◽  
Devendra K Agrawal ◽  
Eric S Peeples
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Injured Brain ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

scholarly journals Directed engineering of umbilical cord blood stem cells to produce C-peptide and insulin

2007 ◽  
Vol 40 (3) ◽  
pp. 367-380 ◽  
Author(s):  
L. Denner ◽  
Y. Bodenburg ◽  
J. G. Zhao ◽  
M. Howe ◽  
J. Cappo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
C Peptide ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Effects of interruptions of controlled-rate freezing on the viability of umbilical cord blood stem cells

Transfusion ◽  
2014 ◽  
Vol 55 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Hongyou Yang ◽  
Alla Pidgorna ◽  
Mona R. Loutfy ◽  
Paul Shuen
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Umbilical Cord Blood Stem Cells Induced into Myeloid, Erythroid, or Megakaryocytic Pathways Are Susceptible to Infection by Porcine Endogenous Retroviruses (PERV).

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4189-4189
Author(s):  
Doris A. Morgan ◽  
Takele Argaw ◽  
Carolyn A. Wilson
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Endogenous Retroviruses ◽  
Nuclear Staining ◽  
Porcine Endogenous Retroviruses ◽  
Cd34 Cells ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Abstract Susceptibility of human hematopoietic stem cells to infection with porcine endogenous retroviruses (PERV) is of prime importance in determining the feasibility and safety of transplanting pig organs into humans. Under the influence of lineage specific cytokines, umbilical cord blood stem cells were evaluated for their permissiveness to PERV prior to linage commitment as well as after induction into the myeloid, erythroid or megakaryocytic pathways. CD34+ cells were plated in serum-containing nutrient medium supplemented with stem cell factor (SCF) to induce proliferation - a requirement for successful retroviral infection. Various microenvironment conditions included addition of lineage specific secondary cytokines for myeloid (G-CSF), erythroid (EPO), and megakaryocytic (TPO) commitment. After seven days, one-half of each culture was exposed to retroviral pseudotypes composed of replication competent wildtype PERV-NIH virions that also carry an MLV-based retroviral vector genome encoding β-galactosidase (β-gal) (Wilson et al, J. Vir,2000); control cultures were not exposed to the virus. Analyses for PERV infection were implemented after 72 hours’ viral exposure with the following observations: (1) exposure to PERV had no detected effect on proliferation nor on terminal differentiation of all three hematopoietic lineages; (2)PERV infected (β-gal +) cells (Fig.1) were detected in all cultures exposed to PERV regardless of microenvironment variations; and (3) PERV reverse transcription (DNA) and gene expression (RNA) as analyzed by PCR were positive only in virus exposed cultures (Fig. 2). Control cultures were negative in all analyses for PERV entry and expression. Preliminary observations indicate the erythroid lineage (SCF+E) may be more efficient in supporting PERV infection than the uninduced CD34+ cells (SCF) as well as those committed to the myeloid (SCF+G) and megakaryocytic (SCF+T) lineages. These results extend the initial observation that PERV could infect established hematopoietic cells lines to now show that the in vitro tropism of PERV also includes primary hematopoietic cells representing three lineages. Fig. 1 Nuclear staining of PERV+ cells. Upper cell is in mitosis Fig. 1. Nuclear staining of PERV+ cells. Upper cell is in mitosis Fig. 2 qPCR Data of PERV-Exposed Cells Fig. 2. qPCR Data of PERV-Exposed Cells

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