scholarly journals Elevated Transforming Growth Factor-β2 in the Aqueous Humor: A Possible Explanation for High Rate of Capsular Contraction Syndrome in High Myopia

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Keke Zhang ◽  
Xiangjia Zhu ◽  
Minjie Chen ◽  
Xinghuai Sun ◽  
Jin Yang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Western Blot ◽  
Aqueous Humor ◽  
Transforming Growth Factor Beta ◽  
High Myopia ◽  
Transforming Growth Factor ◽  
High Rate ◽  
Histopathological Analysis ◽  
Capsular Contraction ◽  
Transforming Growth Factor Β2

Purpose.The purpose of the study was to elucidate the role of transforming growth factor-beta 2 (TGF-β2) in the development of high myopic capsular contraction syndrome.Methods.Nineteen cases of capsular contraction syndrome, including 14 with high myopia, were collected, and their clinical data were reviewed. Aqueous humor and anterior capsular membranes were obtained during capsulotomy. Hematoxylin-eosin and immunohistochemical staining with anti-TGF-β2 antibody were performed on capsular membranes. TGF-β2 levels in aqueous humor were assayed using ELISA and western blot.Results.High myopia was significantly associated with the incidence of capsular contraction syndrome (odds ratio: 14.74,P<0.001, 95% CI: 5.29–41.05). Histopathological analysis revealed proliferation of fibroblast-like lens epithelial cells on the shrunken anterior capsule, labeled with TGF-β2 antibodies. ELISA and Western blot showed higher levels of TGF-β2 in aqueous humor of patients with capsular contraction syndrome and high myopia.Conclusions.High myopia is a risk factor for capsular contraction syndrome. Elevated TGF-β2 levels in high myopic cataract patients may play an important role in the pathogenesis of capsular contraction syndrome.

Transforming growth factor-β2 levels in aqueous humor of glaucomatous eyes

2001 ◽  
Vol 239 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Masaru Inatani ◽  
Hidenobu Tanihara ◽  
Hideto Katsuta ◽  
Megumi Honjo ◽  
Noriaki Kido ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β2

scholarly journals Active transforming growth factor-β2 in the aqueous humor of posterior polymorphous corneal dystrophy patients

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175509 ◽  
Author(s):  
Andrea Stadnikova ◽  
Lubica Dudakova ◽  
Pavlina Skalicka ◽  
Zdenek Valenta ◽  
Martin Filipec ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Transforming Growth Factor ◽  
Corneal Dystrophy ◽  
Posterior Polymorphous Corneal Dystrophy ◽  
Transforming Growth Factor Β2

scholarly journals Author Response: Transforming Growth Factor Beta Switch in Aqueous Humor of Patients With Fuchs' Endothelial Corneal Dystrophy

2016 ◽  
Vol 57 (2) ◽  
pp. 773 ◽  
Author(s):  
Mario Matthaei ◽  
Johannes Gillessen ◽  
Philipp S. Muether ◽  
Robert Hoerster ◽  
Björn O. Bachmann ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Corneal Dystrophy ◽  
Author Response ◽  
Growth Factor Beta

scholarly journals Inhibition of airway remodeling and inflammatory response by Icariin in asthma

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lingli Hu ◽  
Lulu Li ◽  
Hongying Zhang ◽  
Qiuping Li ◽  
Shan Jiang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Western Blot ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Airway Remodeling ◽  
Lavage Fluid ◽  
Airway Smooth Muscle Cell ◽  
High Dose ◽  
Erk Pathway

Abstract Background Icariin (ICA) is the major active ingredient extracted from Chinese herbal medicine Epimedium, which has the effects of improving cardiovascular function, inducing tumor cell differentiation and increasing bone formation. It is still rarely reported that ICA can exert its therapeutic potential in asthma via anti-airway remodeling. The point of the study was to estimate the role of ICA in anti-. airway remodeling and its possible mechanism of action in a mouse ovalbumin. (OVA)-induced asthma model. Methods Hematoxylin and Eosin Staining were performed for measuring airway remodeling related indicators. ELISA, Western blot and Immunohistochemistr-. y (IHC) were used for analyzing the level of protein. RT-PCR was used for analyzing the level of mRNA. Results On days 1 and 8, mice were sensitized to OVA by intraperitoneal injection. From day 16 to day 43, previously sensitized mice were exposed to OVA once daily by nebulizer. Interventions were performed orally with ICA (ICA low, medium and high dose groups) or dexamethasone 1 h prior to each OVA exposure. ICA improves pulmonary function, attenuates pulmonary inflammation and airway remodeling in mice exposed to OVA. Histological and Western blot analysis of the lungs show that ICA suppressed transforming growth factor beta 1 and vascular endothelial growth factor expression. Increase in interleukin 13 and endothelin-1 in serum and bronchoalveolar lavage fluid in OVA-induced asthmatic mice are also decreased by ICA. ICA attenuates airway smooth muscle cell proliferation, as well as key factors in the MAPK/Erk pathway. Conclusions The fact that ICA can alleviate OVA-induced asthma at least partly through inhibition of ASMC proliferation via MAPK/Erk pathway provides a solid theoretical basis for ICA as a replacement therapy for asthma. These data reveal the underlying reasons of the use of ICA-rich herbs in Traditional Chinese Medicine to achieve good results in treating asthma.

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