Effects of Cannabinoid Exposure during Adolescence on the Conditioned Rewarding Effects of WIN 55212-2 and Cocaine in Mice: Influence of the Novelty-Seeking Trait

Adolescent exposure to cannabinoids enhances the behavioural effects of cocaine, and high novelty-seeking trait predicts greater sensitivity to the conditioned place preference (CPP) induced by this drug. Our aim was to evaluate the influence of novelty-seeking on the effects of adolescent cannabinoid exposure. Adolescent male mice were classified as high or low novelty seekers (HNS and LNS) in the hole-board test. First, we evaluated the CPP induced by the cannabinoid agonist WIN 55212-2 (0.05 and 0.075 mg/kg, i.p.) in HNS and LNS mice. Then, HNS and LNS mice were pretreated i.p. with vehicle, WIN 55212-2 (0.1 mg/kg), or cannabinoid antagonist rimonabant (1 mg/kg) and were subsequently conditioned with WIN 55212-2 (0.05 mg/kg, i.p.) or cocaine (1 or 6 mg/kg, i.p.). Only HNS mice conditioned with the 0.075 mg/kg dose acquired CPP with WIN 55212-2. Adolescent exposure to this cannabinoid agonist increased the rewarding effects of 1 mg/kg of cocaine in both HNS and LNS mice, and in HNS mice it also increased the reinstating effect of a low dose of cocaine. Our results endorse a role for individual differences such as a higher propensity for sensation-seeking in the development of addiction.

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Propionic acid induced behavioural effects of relevance to autism spectrum disorder evaluated in the hole board test with rats

Progress in Neuro-Psychopharmacology and Biological Psychiatry ◽

10.1016/j.pnpbp.2019.109794 ◽

2020 ◽

Vol 97 ◽

pp. 109794 ◽

Cited By ~ 5

Author(s):

Melissa M. Meeking ◽

Derrick F. MacFabe ◽

Jennifer R. Mepham ◽

Kelly A. Foley ◽

Lisa J. Tichenoff ◽

...

Keyword(s):

Autism Spectrum Disorder ◽

Propionic Acid ◽

Autism Spectrum ◽

Spectrum Disorder ◽

Behavioural Effects ◽

Board Test ◽

Hole Board ◽

Hole Board Test

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Can the Hole–Board Test Predict a Rat’s Exploratory Behavior in a Free-Exploration Test?

Animals ◽

10.3390/ani11041068 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1068

Author(s):

Wojciech Pisula ◽

Klaudia Modlinska ◽

Katarzyna Goncikowska ◽

Anna Chrzanowska

Keyword(s):

Exploratory Behavior ◽

Predictive Value ◽

Previous Experience ◽

General Hypothesis ◽

Free Exploration ◽

Laboratory Rodents ◽

Board Test ◽

Motivational Mechanism ◽

Hole Board ◽

Hole Board Test

This study focuses on the rat activity in a hole–board setting that we considered a type of exploratory behavior. The general hypothesis is based on the claim that a motivational mechanism is central to both the response to novelty in a highly familiarized environment and the activity in the hole–board apparatus. Our sample consisted of 80 experimentally naive Lister Hooded rats. All rats were tested in the hole–board apparatus. Twenty individuals with the highest hole-board scores and twenty subjects with the lowest hole–board scores subsequently underwent an established free-exploration test. In our study, the scores obtained in the hole–board test had little predictive value for the rats’ activity in the free-exploration test. Based on our previous experience in studying exploratory behavior in the free-exploration test and the data presented in this paper, we suggest that the hole–board test is not an appropriate tool for measuring exploratory behavior in laboratory rodents.

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Neuropharmacological Activity of Various Fractions Obtained from Solanum aethiopicum (Linn.) Fruit in Mice.

Journal of Basic and Social Pharmacy Research ◽

10.52968/27459575 ◽

2020 ◽

Vol 1 (5) ◽

pp. 12-23

Author(s):

A.R. Abubakar ◽

◽

I.H. Sani ◽

S. Malami ◽

A.H. Yaro ◽

...

Keyword(s):

Open Field ◽

Elevated Plus Maze ◽

Toxicity Testing ◽

Aqueous Fraction ◽

Solanum Aethiopicum ◽

Elevated Plus Maze Test ◽

Board Test ◽

Plus Maze ◽

Hole Board ◽

Hole Board Test

Background: Solanum aethiopicum (L.), family Solanaceae, is known as garden eggs. The fruit is used in the treatment of insomnia, diabetes and constipation. Objective: The aim of this study was to evaluate anxiolytic-like activity of fractions obtained from crude methanol extract of Solanum aethiopicum fruit. Method: Acute toxicity testing was conducted according to the OECD guidelines 420 via oral and intraperitoneal routes (ip). n-Hexane (HF), chloroform (CHF), ethyl-acetate (EAF), n-butanol (BF) and residual aqueous fraction (RAF) at doses of 25, 50 and 100 mg/kg ip were experimented using the open field, elevated plus maze, staircase, light dark box and hole-board tests. Results: Results: In open field test, there was statistically significant increase in frequency of central square entry by EAF 25mg/kg, 50mg/kg and 100mg/kg and RAF 25mg/kg, 50mg/kg and 100mg/kg all at p<0.05 compared to distilled water (D/W) group. Elevated plus maze test showed statistically significant increases in open arm entry and duration by CHF 25mg/kg, RAF 25mg/kg and 50mg/kg again at p<0.05. Also, in the staircase test, statistically significant decrease in frequency of rearing with no effect on step climbing was observed by RAF 25mg/kg (p< 0.05) compared to D/W. Light and dark box test produced increased light box entry and duration by EAF 25mg/kg, RAF 25mg/kg and 50mg/ kg at p<0.05. Furthermore, the hole -board test showed statistically significant increases in number of head dips by EAF 50mg/kg and 100mg/kg as well as RAF 25mg/kg, 50mg/kg and 100 mg/kg at p<0.05. Conclusion: The fractions obtained from Solanum aethiopicum fruits possesses anxiolytic-like activity.

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Interference of propylene glycol with the hole-board test

Brazilian Journal of Medical and Biological Research ◽

10.1590/s0100-879x2001000400016 ◽

2001 ◽

Vol 34 (4) ◽

pp. 545-547 ◽

Cited By ~ 13

Author(s):

A. Lourenço da Silva ◽

E. Elisabetsky

Keyword(s):

Propylene Glycol ◽

Board Test ◽

Hole Board ◽

Hole Board Test

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Evaluation of anxiolytic and hypoglycemic potential of Cissus adnata Roxb. in animal model

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2018-0130 ◽

2019 ◽

Vol 17 (2) ◽

Author(s):

Mohammed Shamim Hasan ◽

Md. Giash Uddin ◽

Mohammed Shoibe ◽

Abdullah Al Mahmud ◽

Sujan Banik

Keyword(s):

Glucose Tolerance Test ◽

Methanolic Extract ◽

Anxiolytic Effect ◽

Tolerance Test ◽

Mice Model ◽

Board Test ◽

Plus Maze ◽

Hole Board ◽

Phytochemical Constituents ◽

Hole Board Test

AbstractBackgroundThis study was designed to evaluate the anxiolytic and hypoglycemic potential of methanolic extract of Cissus adnata Roxb. is a crucial medicinal plant used in many disorders belongs to Vitaceae family.MethodsElevated plus maze (EPM) test and hole board test was applied for the anxiolytic activity with the Swiss albino mice. The hypoglycemic activity was measured by the glucose tolerance test in mice model. The capacity to produce the desired effect of the plant extract (200 and 400 mg/kg) was compared with the anxiolytic drug of standard diazepam (1 mg/kg i.p.) and anti-diabetic drug glibenclamide (10 mg/kg i.p.), respectively.ResultsThe phytochemical screening of Cissus adnata extract exposed the presence of carbohydrate, phenol, flavonoid, saponins, cardiac glycoside, tannin, and gum. The anxiolytic effect was detected in both experiments which significantly raised the number of head dips and the time spent in the open arm of the EPM (p<0.05) as the dose enlarged. Hypoglycemic study of the extracts shows better effect by reducing blood glucose level.ConclusionsThe better anxiolytic and hypoglycemic activities in the present study are due to the existence of various phytochemical constituents like saponins, flavonoids, terpenoids, phenols, and tannins in this methanolic extract.

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