scholarly journals Inhibitory Activities ofStauntonia hexaphyllaLeaf Constituents on Rat Lens Aldose Reductase and Formation of Advanced Glycation End Products and Antioxidant

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Seung Hwan Hwang ◽  
Shin Hwa Kwon ◽  
Set Byeol Kim ◽  
Soon Sung Lim
Keyword(s):  
Aldose Reductase ◽  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Rat Lens Aldose Reductase ◽  
Glycation End Products ◽  
End Products ◽  
Etoac Fraction

Stauntonia hexaphylla(Thunb.) Decne. (Lardizabalaceae) leaves (SHL) have been used traditionally as analgesics, sedatives, diuretics, and so on, in China. To date, no data have been reported on the inhibitory effect of SHL and its constituents on rat lens aldose reductase (RLAR) and advanced glycation end products (AGEs). Therefore, the inhibitory effect of compounds isolated from SHL extract on RLAR and AGEs was investigated to evaluate potential treatments of diabetic complications. The ethyl acetate (EtOAC) fraction of SHL extract showed strong inhibitory activity on RLAR and AGEs; therefore, EtOAc fraction (3.0 g) was subjected to Sephadex LH-20 column chromatography, for further fractionation, with 100% MeOH solvent system to investigate its effect on RLAR and AGEs. Phytochemical investigation of SHL led to the isolation of seven compounds. Among the isolated compounds, chlorogenic acid, calceolarioside B, luteolin-3′-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, and luteolin-7-O-β-D-glucopyranoside exhibited significant inhibitory activity against RLAR with IC50in the range of 7.34–23.99 μM. In addition, 3-(3,4-dihydroxyphenyl) propionic acid, neochlorogenic acid, and luteolin-3′-O-β-D-glucopyranoside exhibited the most potent inhibitory activity against formation of AGEs, with an IC50value of 115.07–184.06 μM, compared to the positive control aminoguanidine (820.44 μM). Based on these findings, SHL dietary supplements could be considered for the prevention and/or treatment of diabetes complication.

scholarly journals Aldose reductase and advanced glycation end products formation inhibitory activity of standardized extracts of Picrorhiza kurroa (Royle ex benth) and Hibiscus rosa-sinensis (Linn.)

2017 ◽  
Author(s):  
Anasuri Santhosh ◽  
C. Veeresham ◽  
A. Rama Rao
Keyword(s):  
Aldose Reductase ◽  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Reference Compound ◽  
Advanced Glycation ◽  
Hibiscus Rosa Sinensis ◽  
Glycation End Products ◽  
End Products

Objective: The objectives were to study the in-vitro and in-vivo aldose reductase and in-vitro advanced glycation end products formation inhibitory activities of the standardized extracts of Picrorhiza kurroa roots and Hibiscus rosa-sinensis flowers.Methods: In-vitro Aldose reductase inhibitory activity was studied by using isolated rat lens and kidney Aldose reductase by UV-Visible spectro photo metric method by using Quercetin as reference compound. In-vivo Aldose reductase inhibitory activity was evaluated by using experimental rat models of galactosemia and the final lens galactitol was evaluated by High performance liquid chromatography and Gas chromatography methods by using Quercetin as reference compound. In-vitro advanced glycation end products formation inhibitory activity was estimated by using laboratory test reaction with protein and sugars by spectro fluorimetric method by sung aminoguanidine as reference compound. Statistical analysis of the results was done by using Analysis of the variance method.Results: The plant extracts were found to possess significant aldose reductase and advanced glycation end products formation inhibitory activity.Conclusions: More study is required for isolation and characterization of the chief chemical constituents responsible for the biological activity of the plant extracts.

scholarly journals Inhibitory Effect of Several Mangifera indica Cultivar Leaf Extracts on the Formation of Advanced Glycation End Products (AGEs)

2020 ◽  
Vol 9 (2) ◽  
pp. 33
Author(s):  
Kimihisa Itoh ◽  
Tetsuya Matsukawa ◽  
Kanasa Minami ◽  
Mamoru Okamoto ◽  
Norimichi Tomohiro ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Mangifera Indica ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Indica Cultivar

As a part of our ongoing research to find novel functions in mango leaves, we have reported that the methanolic extract of pruned old dark green mango leaf (Mangifera indica ‘Irwin’) exhibited inhibitory effects on the formation of advanced glycation end products (AGEs) in nonenzymatic glycation of albumin. The purpose of this study was to find other mango cultivars with more potent activity in this regard. We examined the inhibitory effect of seventeen mango (Mangifera indica) cultivar leaf extracts on AGEs formation. We also investigated the relationship between the inhibitory activity of the extracts and the contents of their active components, 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll (3). On the basis of the evaluation of the inhibitory activity of mango cultivar leaf extracts, the HPLC determination of the contents of 1 and 2, and the spectrophotometric determination of 3, it was found that almost all extract showed a significant activity, and the content of 2 and 3 detected in each was similar. In contrast, AGEs formation inhibition tended to be higher as the content of 1 in the leaf extracts increased. This is the first report of phytochemical analysis of compounds 1, 2 and 3 in various cultivars of mango leaf. From the phytochemical point of view, these results suggest that the pruned leaves of any cultivar of Mangifera indica except ‘Chiin Hwang No. 1’ and ‘Kyo Savoy’ may be useful for the preparation of natural ingredients with inhibitory activity of AGEs formation.

scholarly journals Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract

2017 ◽  
Vol 6 (2) ◽  
pp. 102 ◽  
Author(s):  
Kimihisa Itoh ◽  
Kazuya Murata ◽  
Nao Sakaguchi ◽  
Kohei Akai ◽  
Tomoka Yamaji ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Mangifera Indica ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Dark Green

The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.

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