Preliminary Study on the Tears Oxidative Stress Status and Sleep Disturbances in Irritable Bowel Syndrome Patients

According to the latest gastrointestinal disorders diagnostic criteria (ROME IV), the irritable bowel syndrome (IBS) is mainly characterized by the presence of abdominal pain and changes in intestinal transit. However, both sleep impairments and oxidative status changes (in patients’ sera, mucosal level, and other body fluids) were reported IBS. Thus, in this study, we aimed to evaluate several aspects regarding the oxidative stress status in patients’ tears as well as sleep disturbances by comparison with the intensity of IBS symptoms, as assessed by the visual analogue scale for irritable bowel syndrome (VAS-IBS). Ten IBS patients and fourteen healthy sex- and age-matched volunteers were recruited from the Oftaprof Ophthalmological Clinic (Iași, Romania). Visual analogue scale for irritable bowel syndrome and the Pittsburgh Sleep Quality Index (PSQI) questionnaires were administered to all the patients. Tear samples were collected using the Schirmer test procedure and were subjected to biochemical analysis—superoxide dismutase and glutathione peroxidase activities, malondialdehyde, and total soluble proteins levels were determined. Standard statistical analysis was applied. We found significant differences in oxidative stress marker dynamics in IBS patients as compared to healthy age- and sex-matched controls: increased superoxide dismutase activity (p=0.02), increased malondialdehyde (p=0.007), and total soluble proteins levels (p=0.019). We found no significant differences in tear glutathione peroxidase activity in IBS patients as compared to healthy age- and sex-matched controls (p=0.55). Furthermore, we observed that the oxidative stress tear markers are correlated with gastrointestinal symptoms severity (as evaluated by VAS-IBS) but not correlated to the sleep quality index and items (as evaluated by PSQI), with significant differences according to patient sex and IBS subtype stratification. In this way, this study brings additional evidence of the oxidative stress role in IBS pathology alongside the evaluation of tear fluid molecular dynamics in IBS for the first time in our best knowledge.

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The brief Visual Analogue Scale for Irritable Bowel Syndrome questionnaire can be used to evaluate psychological well-being in patients with irritable bowel syndrome

European Journal of Internal Medicine ◽

10.1016/j.ejim.2013.05.013 ◽

2013 ◽

Vol 24 (7) ◽

pp. e82-e83 ◽

Cited By ~ 5

Author(s):

Mariette Bengtsson ◽

Bodil Ohlsson

Keyword(s):

Irritable Bowel Syndrome ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Well Being ◽

Psychological Well Being ◽

Irritable Bowel

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Development and psychometric testing of the Visual Analogue Scale for Irritable Bowel Syndrome (VAS-IBS)

BMC Gastroenterology ◽

10.1186/1471-230x-7-16 ◽

2007 ◽

Vol 7 (1) ◽

Cited By ~ 60

Author(s):

Mariette Bengtsson ◽

Bodil Ohlsson ◽

Kerstin Ulander

Keyword(s):

Irritable Bowel Syndrome ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Psychometric Testing ◽

Irritable Bowel

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Further Validation of the Visual Analogue Scale for Irritable Bowel Syndrome After Use in Clinical Practice

Gastroenterology Nursing ◽

10.1097/sga.0b013e3182945881 ◽

2013 ◽

Vol 36 (3) ◽

pp. 188-198 ◽

Cited By ~ 19

Author(s):

Mariette Bengtsson ◽

Jesper Persson ◽

Kristina Sjölund ◽

Bodil Ohlsson

Keyword(s):

Irritable Bowel Syndrome ◽

Clinical Practice ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Irritable Bowel

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Preliminary Biochemical Description of Brain Oxidative Stress Status in Irritable Bowel Syndrome Contention-Stress Rat Model

Medicina ◽

10.3390/medicina55120776 ◽

2019 ◽

Vol 55 (12) ◽

pp. 776 ◽

Cited By ~ 3

Author(s):

Ioana-Miruna Balmus ◽

Radu Lefter ◽

Alin Ciobica ◽

Sabina Cojocaru ◽

Samson Guenne ◽

...

Keyword(s):

Oxidative Stress ◽

Nervous System ◽

Irritable Bowel Syndrome ◽

Rat Model ◽

Complex Model ◽

Control Group ◽

Oxidative Stress Status ◽

Male Wistar Rats ◽

Brain Oxidative Stress ◽

Irritable Bowel

Background and objectives: Oxidative stress and inflammation have been implicated in the etiology of irritable bowel syndrome (IBS), a common gastrointestinal functional disease. This study aimed to further characterize the contention-stress rat model by exploring a possible correlation between oxidative stress markers measured in brain tissues with behavioral components of the aforementioned model. Thus, it is hereby proposed a possible IBS animal model relevant to pharmacological and complementary medicine studies. Materials and Methods: Wild-type male Wistar rats (n = 5/group) were chronically exposed to 6-hour/day contention, consisting of isolating the animals in small, vital space-granting plastic devices, for seven consecutive days. Following contention exposure, temporal lobes were extracted and subjected to biochemical analyses to assess oxidative stress-status parameters. Results: Our results show increased brain oxidative stress in contention-stress rat model: decreased superoxide dismutase and glutathione peroxidase activities and increased malondialdehyde production in the IBS group, as compared to the control group. Furthermore, the biochemical ratios which are used to evaluate the effectiveness of an antioxidant system on oxidative stress could be described in this model. Conclusions: The correlations between the behavioral patterns and biochemical oxidative stress features could suggest that this may be a complex model, which can successfully mimic IBS symptomatology further providing evidence of a strong connection between the digestive system, enteric nervous system, and the central nervous system.

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Evaluation of gastrointestinal symptoms in different patient groups using the visual analogue scale for irritable bowel syndrome (VAS-IBS)

BMC Gastroenterology ◽

10.1186/1471-230x-11-122 ◽

2011 ◽

Vol 11 (1) ◽

Cited By ~ 21

Author(s):

Mariette Bengtsson ◽

Oskar Hammar ◽

Thomas Mandl ◽

Bodil Ohlsson

Keyword(s):

Irritable Bowel Syndrome ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Gastrointestinal Symptoms ◽

Patient Groups ◽

Irritable Bowel

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Validation of the Korean version of visual analogue scale for irritable bowel syndrome questionnaire for assessment of defecation pattern changes

Annals of Surgical Treatment and Research ◽

10.4174/astr.2018.94.5.254 ◽

2018 ◽

Vol 94 (5) ◽

pp. 254

Author(s):

Hong Yeol Yoo ◽

Boram Park ◽

Jungnam Joo ◽

Jung-Sup Kim ◽

Yumi Lee ◽

...

Keyword(s):

Irritable Bowel Syndrome ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Korean Version ◽

Irritable Bowel

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Evaluation of Non-Celiac Gluten Sensitivity in Patients with Previous Diagnosis of Irritable Bowel Syndrome: A Randomized Double-Blind Placebo-Controlled Crossover Trial

Nutrients ◽

10.3390/nu12030705 ◽

2020 ◽

Vol 12 (3) ◽

pp. 705 ◽

Cited By ~ 2

Author(s):

Michele Barone ◽

Eugenio Gemello ◽

Maria Teresa Viggiani ◽

Fernanda Cristofori ◽

Caterina Renna ◽

...

Keyword(s):

Irritable Bowel Syndrome ◽

Visual Analogue Scale ◽

Analogue Scale ◽

Challenge Test ◽

Gluten Sensitivity ◽

Gluten Free ◽

Double Blind ◽

Previous Diagnosis ◽

Irritable Bowel ◽

Fodmap Diet

Background. To date, there is no reliable marker for the diagnosis of non-celiac gluten sensitivity (NCGS), which benefits from a gluten-free diet (GFD). This condition is characterized by functional gastrointestinal symptoms similar to those occurring in the course of irritable bowel syndrome (IBS). However, IBS has a higher prevalence, and often benefits from the administration of a low fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAP) diet. The overlap of symptoms between these two pathologies has led to an overestimation of self-made diagnosis NCGS. Aims. To better identify NCGS in subjects with a previous diagnosis of IBS. Methods. All subjects received a low FODMAP diet that was also gluten-free (low FODMAP-GFD), and those presenting an improvement of symptoms were exposed to gluten or placebo (double-blind challenge with wash-out and crossover). The response to dietary treatments was evaluated by visual analogue scale (VAS). Results. Of 30 patients (23 women, seven men, aged 42.2 ± 12.5 years, body mass index (BMI) 24.7 ± 4.1 kg/m2), 26 benefited from the administration of low FODMAP-GFD and were exposed to the gluten/placebo challenge. After the challenge, using an increase of visual analogue scale VAS (Δ-VAS) ≥30%, 46.1% of the patients were NCGS+. However, this percentage became only 19.2% using a different method (mean ∆-VAS score plus two standard deviations). Conclusions. FODMAP intolerance could hide the response to a challenge test with gluten for the identification of NCGS in IBS patients. A low FODMAP-GFD followed by gluten/placebo challenge is able to identify patients with NCGS better. ClinicalTrials.gov registration number NCT04017585.

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Antioxidant Capacity and Behavioral Relevance of a Polyphenolic Extract of Chrysanthellum americanum in a Rat Model of Irritable Bowel Syndrome

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/3492767 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 5

Author(s):

Roxana Cojocariu ◽

Alin Ciobica ◽

Ioana-Miruna Balmus ◽

Samson Guenne ◽

Anca Trifan ◽

...

Keyword(s):

Oxidative Stress ◽

Irritable Bowel Syndrome ◽

Rat Model ◽

Specific Activity ◽

Polyphenolic Extract ◽

Y Maze ◽

Oxidative Stress Status ◽

Cognitive Disturbances ◽

Enzyme Specific Activity ◽

Irritable Bowel

Chrysanthellum americanum L. (Vatke) is a medicinal plant from the Compositae family used in west-African traditional medicine, known for its flavonoid and saponin richness and for its strong antioxidant potential. In the present study, we assessed the effects of Chrysanthellum americanum polyphenolic extract in the psychological stress-induced rat model of irritable bowel syndrome (IBS), a chronic functional digestive tract disorder marked by immune and inflammatory-related disturbances of central nervous and peripheral intestinal systems, which is often associated with mood disorders including depression and anxiety. Consequently, memory impairment, anxiety and depression behavioral indicators, and cerebral oxidative stress biomarker dynamics were evaluated in a multifactorial heterotypic stress-exposed IBS rats after 6-day gavage with polyphenolic C. americanum extract (100 mg/kg body weight). Y-maze, elevated plus maze, and forced swimming tests were used for assessing behavioral responses. Administration of the extract exhibited significant anxiolytic and antidepressant-like effects coupled with significantly increased temporal lobe antioxidant enzyme specific activity (superoxide dismutase and glutathione peroxidase) and decreased malondialdehyde levels, a well-known lipid peroxidation marker. Furthermore, linear regression statistical analyses showed significant correlations between the oxidative stress parameters and behavioral tests. In conclusion, our results suggest that the administration of Chrysanthellum americanum polyphenolic extract could ameliorate mood and cognitive disturbances related to stress-induced in an IBS rat model. This could be also related to cerebral oxidative stress status attenuation.

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