scholarly journals Micropropagation of Livingstone Potato (Plectranthus esculentus N.E.Br)

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
G. T. Kujeke ◽  
T. C. Chitendera ◽  
R. T. Masekesa ◽  
U. Mazarura ◽  
E. Ngadze ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Block Design ◽  
Growth Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Explant Type ◽  
Planting Material ◽  
Two Factors

Livingstone potato (Plectranthus esculentus N.E.Br) is an underutilised indigenous root vegetable grown by communal farmers in the eastern provinces of Zimbabwe. It is vegetatively propagated using unimproved retained tubers from the previous season. The risk of disease carryover is therefore high, leading to poor yields. The objective of the study was to exploit the tissue culture technique of micropropagation to produce a mass supply of healthy planting material for improved productivity. Two experiments were conducted: firstly, to determine the best explant type and secondly, to determine the best landrace and plant growth regulators for the growth of plantlets. The landraces, namely, Ndurwe, Musande, Chibanda, and Chizambezi, were sourced from communal farmers in the stated production areas. Naphthalene acetic acid (NAA) and benzyl amino purine (BAP) were the auxin and cytokinin used, respectively. The first experiment was laid out as a randomized complete block design (RCBD) with two factors: landrace and explant type (shoot tips, nodes, and leaves). After culturing the explants on a plain Murashige Skoog (MS) medium for ten weeks, the best explant was the node with regards to the number of nodes, shoots, and roots of the plantlets which were significant (P<0.05). The second experiment was laid out as a RCBD with two factors: landraces and the plant growth regulator combinations. The nodes were subcultured on an MS medium supplemented with the 16 combinations of plant growth regulators (0 mg/l, 0.5 mg/l, 1 mg/l, and 2 mg/l BAP concentrations: 0 mg/l, 0.2 mg/l, 0.5 mg/l, and 1 mg/l NAA concentrations), respectively. Chizambezi performed best and is, therefore, highly recommended for the rapid multiplication of Livingstone potato. Results from this study have clearly demonstrated that the addition of NAA: BAP at varying concentrations was significant and is essential for optimizing the growth media for micropropagation of Livingstone potato in Zimbabwe. Commercial production of plantlets can, therefore, be carried out to provide healthy planting material for the communal farmers for improved productivity while preserving the germplasm of the underutilised crop at the same time.

scholarly journals Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.)

2014 ◽  
Vol 32 (2) ◽  
pp. 170-179 ◽  
Author(s):  
Hernando Criollo ◽  
Margarita Perea ◽  
Mariano Toribio ◽  
Johanna Muñoz
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Plant Propagation ◽  
Cotyledonary Leaf

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 mM of NAA were added to the medium with sucrose (50.0 and 263.1 mM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 mM of NAA ; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 mM of NAA + 263.1 mM of sucrose (32%).

scholarly journals EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Khierallah & Al-Obaidy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Control Treatment ◽  
Ms Medium ◽  
Explant Type ◽  
Culture Initiation ◽  
The Impact

This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).  The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.

scholarly journals Impact of Plant Growth Regulators on Greek oregano Micropropagation and Antioxidant Activity

2016 ◽  
Vol 16 (2) ◽  
pp. 297-305
Author(s):  
Ely Georgieva Zayova ◽  
Maria Prokopova Geneva ◽  
Kamelia Dimitrova Miladinova-Georgieva ◽  
Marieta Georgieva Hristozkova ◽  
Ira Valkova Stancheva
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Butyric Acid ◽  
Growth Regulators ◽  
Multiple Shoots ◽  
Antioxidant Potential ◽  
Naphthalene Acetic Acid ◽  
Average Height ◽  
Ms Medium

This study highlights the development and achievements made for the micropropagation of Greek oregano (Origanum heracleoticum L. ) using stem tip explants. The shoots were cultured on Murashige and Skoog (MS) medium followed different concentrations of plant growth regulators (PGR) - 6-benzyl aminopurine, thidiazuron and zeatin at concentrations (0.5 or 1.0 mg L-1). The induction of multiple shoots from stem tip segments was the highest in MS medium supplemented with 1.0 mg L-1 zeatin. It was the most effective medium for shoot formation, which produced multiple shoots (2.7) with an average height of 3.5 cm. These shoots were transferred on half strength MS medium containing three different auxins: indole-3-butyric acid, α-naphthalene acetic acid or indole-3-acetic acid (0, 0.1 and 0.5 mg L-1) for rooting, Multiple shoots were the most efficiently rooted on ½ MS medium supplemented with 0.5 mg L-1indole-3-butyric acid. Rooted plants showed the best adaptation on pots containing peat: perlite (2: 1 v/v). The higher rates of shoots number and height per plant have a positive relationship with the production of metabolites with antioxidant potential as phenols and flavonoids as well as with ferric reducing antioxidant potential.

scholarly journals Influence of growth regulators on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 3) ◽  
pp. 118-122
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová ◽  
Z. Jureková
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Explant Type ◽  
Regeneration Capacity

We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm<sup>3</sup> zeatin and 0.1 mg/dm<sup>3</sup> IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90&ndash;92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. &nbsp;

Callogenesis and plant regeneration of sweet orange cv. Washington Navel from floral organ cultures

2020 ◽  
pp. 19-23
Author(s):  
Nebiha Metoui ◽  
Sabrine Nahdi ◽  
Fethia Dhaouadi ◽  
Dorsaf Yahiaoui ◽  
Malika Meziane
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Floral Organ ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Washington Navel ◽  
2,4 Dichlorophenoxyacetic Acid

Washington Navel orange (Citrus sinensis L.) can be infected with virus and virus like diseases that affect not only the production but also fruit quality and the plant’s longevity. For viral sanitation, Washington Navel regeneration was investigated in vitro via floral organ culture. Flowers were collected before opening from healthy Washington Navel trees kept under greenhouse. Floral organs (style/stigma and ovary) were cultured on Murashige and Skoog (MS) medium containing various plant growth regulators combinations of naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The highest rate of callogenesis (95%) was obtained from style/stigma explant cultures on MS medium enriched with 3 mgL-1 BAP, which also resulted in 100% rooted plantlets. Ovary cultures did not show any success on the culture medium with various plant growth regulators combinations. The acclimatization success of rooted plantlets by grafting on Citrus volkameriana rootstocks was about 83%. Thus, these results can be used for mass production of disease-free citrus plants and improve sanitation program of the local citrus genotypes in Tunisia.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Effect of Plant Growth Regulators on Growth and Quality Flower Production of Chrysanthemum (Chrysanthemum Indicum L.)

2022 ◽  
Vol 2 (1) ◽  
pp. 10-18
Author(s):  
Md Ehsanullah ◽  
Ahasan Ullah Khan ◽  
Md Kamruzzam ◽  
Sarah Tasnim
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Block Design ◽  
Research Centre ◽  
Vase Life ◽  
Flower Initiation ◽  
Growth Promoter ◽  
Flower Production ◽  
Minimum Number

A field study was conceded to assess the effect of plant growth regulators on growth and quality flower production of chrysanthemum at Horticulture Research Centre (HRC), Gazipur, Bangladesh. The experiment was laid out in Randomized Complete Block Design (RCBD) with ten (10) treatments and three replications. The treatments of plant growth regulators concentration were T1-50 ppm GA3, T2-100 ppm GA3, T3-150 ppm GA3, T4-400 ppm CCC, T5-600 ppm CCC, T6-800 ppm CCC, T7-250 ppm MH, T8-500 ppm MH, T9-750 ppm MH and, T10-Control. The maximum spreading of plant (27.0 cm) was observed when plants were treated with GA3 @ 150 ppm where the minimum plant spread (16.8 cm) was recorded in plants treated with CCC @ 800 ppm. The higher number of suckers (33) per pot was produced when pots were treated with GA3 @ 150 ppm whereas, application of CCC at three different concentrations produced lower number of suckers.  The highest number of flower (40) was recorded with 150 ppm GA3, where minimum number of flowers (25) per pot in 800 ppm CCC. The plants sprayed with 50 ppm GA3 took 48 days to flower initiation, whereas, it took 70 days with 750 ppm MH. the highest plants recorded (7.40 cm) with 800 ppm CCC, whereas, lowest size (6.50 cm) was obtained with the application of 500 ppm MH. The maximum vase life of flowers was recorded for the treatment 800 ppm CCC (15 days), which was at par with 13 days vase life obtained by spraying 600 ppm CCC. Therefore, it is concluded that the GA3 acted as growth promoter and the CCC acted as growth retardants on yield and quality of chrysanthemum.

scholarly journals VERSATILE ROLE OF AUXIN AND ITS CROSSTALK WITH OTHER PLANT HORMONES TO REGULATE PLANT GROWTH AND DEVELOPMENT

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hammad Ishtiaq ◽  
Savita Bhardwaj ◽  
Aaliya Ashraf ◽  
Dhriti Kapoor
Keyword(s):  
Cell Cycle ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Hormones ◽  
Growth And Development ◽  
Naphthalene Acetic Acid ◽  
Plant Growth And Development ◽  
Indole Butyric Acid ◽  
Cellular Processes

Plant growth regulators are significant chemical compounds which are synthesized inside the plant cells and play vital role in plant growth and development. Such compounds are usually active at very low concentrations. These plant growth regulators act as a signalling molecule, which influences the growth of plants. Throughout the previous year’s remarkable investigation have been done for understanding the synthesis of auxin and its effect on various physiological progressions. Auxin is a plant hormone that is involved in various physiological activities, including basic cellular processes such as cell enlargement, regulation of the cell cycle and distinction progress. Plants and several other microorganisms together produce auxin in order to carry out their cell cycle. The chemically synthesized auxins like NAA (naphthalene acetic acid) and IBA (Indole- butyric acid), also take part in various cellular processes. Against various types of biotic and abiotic stress conditions, these plant hormones significantly contribute in promoting acclimatization and adaptation in combination with other phytohormones. The present review highlights some of the important features of auxin role in regulation of plant growth either alone or in crosstalk with other plant hormones.

scholarly journals Influence of size and plant growth regulators on corm and cormel production of gladiolus (Gladiolus grandiflorus L.)

2018 ◽  
Vol 29 (2) ◽  
pp. 91-98
Author(s):  
MHA Rashid
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Tap Water ◽  
Block Design ◽  
Cut Flower ◽  
Combined Treatments ◽  
Maximum Weight ◽  
Major Constraint ◽  
Breaking Dormancy

Gladiolus is an excellent cut flower grown throughout the world for its spikes with florets of massive form, brilliant colours, attractive shapes, varying size and long shelf life. However, major constraint for gladiolus cultivation is the corm dormancy. Plant growth regulators (PGRs) play an important role in breaking dormancy and promote more number of quality corm and cormel productions in gladiolus. Therefore, an experiment was conducted to study the influence of corm size and plant growth regulators on corm and cormel production of gladiolus during the period from October 2017 to April 2018 at the Landscaping section of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh. The two-factor experiment included two corm sizes viz., 3-4 cm and 4.1-5 cm, and two PGRs viz., GA3 @ 250, 500 and 750 ppm; and NAA @ 100, 200 and 300 ppm along with tap water as control. The experiment was laid out in a randomised complete block design (RCBD) with three replications. The results revealed that the corm size and PGRs at different concentrations significantly influenced the corm and cormel producing attributes of gladiolus. Maximum number of corms and cormels per plant, maximum weight of single corm, maximum weight of corms and cormels per plant, biggest size of single corm, highest yield of corms and cormels per hectare were recorded from 4.1-5 cm sized corms and GA3 @ 500 ppm, compared to rest of the treatments. It was observed that combined treatments had significant influence on all the parameters studied. The treatment combination of 4.1-5 cm sized corms and GA3 @ 500 ppm was found to be best in terms of corm and cormel production of gladiolus.Progressive Agriculture 29 (2): 91-98, 2018

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