scholarly journals Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Zhanglong Peng ◽  
Supinder Bedi ◽  
Vivek Mann ◽  
Alamelu Sundaresan ◽  
Kohei Homma ◽  
...  

To mimic Alzheimer’s disease, transgenic mice overexpressing the amyloid precursor protein (APP) were used in this study. We hypothesize that the neuroprotective effects of ETAS®50, a standardized extract of Asparagus officinalis stem produced by Amino Up Co., Ltd. (Sapporo, Japan), are linked to the inhibition of the apoptosis cascade through an enhancement of the stress-response proteins: heat shock proteins (HSPs). APP-overexpressing mice (double-transgenic APP and PS1 mouse strains with a 129s6 background), ages 6-8 weeks old, and weighing 20-24 grams were successfully bred in our laboratory. The animals were divided into 5 groups. APP-overexpressing mice and wild-type (WT) mice were pretreated with ETAS®50 powder (50% elemental ETAS and 50% destrin) at 200 mg/kg and 1000 mg/kg body weight. Saline, the vehicle for ETAS®50, was administered in APP-overexpressing mice and WT mice. ETAS®50 and saline were administered by gavage daily for 1 month. Cognitive assessments, using the Morris Water Maze, demonstrated that memory was recovered following ETAS®50 treatment as compared to nontreated APP mice. At euthanization, the brain was removed and HSPs, amyloid β, tau proteins, and caspase-3 were evaluated through immunofluorescence staining with the appropriate antibodies. Our data indicate that APP mice have cognitive impairment along with elevated amyloid β, tau proteins, and caspase-3. ETAS®50 restored cognitive function in these transgenic mice, increased both HSP70 and HSP27, and attenuated pathogenic level of amyloid β, tau proteins, and caspsase-3 leading to neuroprotection. Our results were confirmed with a significant increase in HSP70 gene expression in the hippocampus.

2002 ◽  
Vol 19 (5) ◽  
pp. 601-614 ◽  
Author(s):  
James R. Stone ◽  
David O. Okonkwo ◽  
Richard H. Singleton ◽  
Leman K. Mutlu ◽  
Gregory A. Helm ◽  
...  

2013 ◽  
Vol 1 (1) ◽  
pp. 77 ◽  
Author(s):  
Ajeet Rijal Upadhaya ◽  
Frederik Scheibe ◽  
Irina Kosterin ◽  
Dorothee Abramowski ◽  
Janina Gerth ◽  
...  

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159119 ◽  
Author(s):  
Isobel J. Whitehouse ◽  
Deborah Brown ◽  
Herbert Baybutt ◽  
Abigail B. Diack ◽  
Katherine A. B. Kellett ◽  
...  

2010 ◽  
Vol 116 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Beatriz G. Perez Nievas ◽  
Thea Hammerschmidt ◽  
Markus P. Kummer ◽  
Dick Terwel ◽  
Juan C. Leza ◽  
...  

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