Relationship between HCV RNA quantities and HCV genotypes and the outcome of patients with posttransfusion hepatitis C virus infection

The aims of this study were to (i) evaluate the prevalence and the incidence of hepatitis C virus (HCV) infection in hemodialysis patients in two different centers in São Paulo (Brazil), (ii) determine the time required to detect HCV infection among these patients by serology or PCR, (iii) establish the importance of alanine aminotransferase determination as a marker of HCV infection, and (iv) identify the HCV genotypes in this population. Serum samples were collected monthly for 1 year from 281 patients admitted to hospital for hemodialysis. Out of 281 patients, 41 patients (14.6%) were HCV positive; six patients seroconverted during this study (incidence = 3.1/1000 person-month). In 1.8% (5/281) of cases, RNA was detected before the appearance of antibodies (up to 5 months), and in 1.1% (3/281) of cases, RNA was the unique marker of HCV infection. The genotypes found were 1a, 1b, 3a, and 4a. The presence of genotype 4a is noteworthy, since it is a rare genotype in Brazil. These data pointed out the high prevalence and incidence of HCV infection at hemodialysis centers in Brazil and showed that routine PCR is fundamental for improving the detection of HCV carriers among patients undergoing hemodialysis.Key words: HCV genotypes, hemodialysis, hepatitis C, PCR, prevalence, incidence.

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Hepatitis C Virus Infection in Pregnant Women: Detection by Different Anti-HCV Immunoassays and Serum HCV-RNA

Asia-Oceania Journal of Obstetrics and Gynaecology ◽

10.1111/j.1447-0756.1994.tb00414.x ◽

2010 ◽

Vol 20 (1) ◽

pp. 13-18 ◽

Cited By ~ 1

Author(s):

Ho-Hsiung Lin ◽

Hong-Yuan Hsu ◽

Tzu-Yao Lee ◽

Jia-Horng Kao ◽

Pei-Jer Chen ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Virus Infection ◽

Pregnant Women ◽

Hcv Rna ◽

Hepatitis C Virus Infection

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Occult hepatitis C virus infection in patients with malignant lymphoproliferative disorders

International Journal of Immunopathology and Pharmacology ◽

10.1177/2058738420961202 ◽

2020 ◽

Vol 34 ◽

pp. 205873842096120

Author(s):

Abeya A Lotfi ◽

Asmaa E Mohamed ◽

Nahela A Shalaby ◽

Deena S Eissa ◽

Ehab El-Dabaa ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Virus Infection ◽

Treatment Protocol ◽

Lymphoproliferative Disorders ◽

Hcv Infection ◽

Hcv Rna ◽

Hepatitis C Virus Infection ◽

Occult Hepatitis ◽

Occult Hepatitis C

Despite the link between HCV and malignant lymphoproliferative disorders has been established, the association between occult hepatitis C virus infection and malignant lymphoproliferative disorders remains obscure. The present study intended to identify the possible association between occult HCV infection and malignant lymphoproliferative disorders. Newly diagnosed patients with LPDs were screened for the presence of HCV-RNA in both plasma and PBMCs. PBMCs of the subjects were also, examined by transmission and immuno-electron microscopy. LPD patients showed a high percentage of HCV infection (71.9%): OCI-HCV (37.5%) and HCV (34.38%). Meanwhile, 28.13% of LPD patients did not show any evidence of HCV infection. Ultrastructural examination of PBMCs revealed the presence of intracytoplasmic vacuoles enclosing viral like particles, which were less prominent in occult HCV patients. The possibility of occult HCV should be considered in patients with LPDs which can be helpful in the management of the treatment protocol in order to set up a balance between the control of the tumor progression and minimizing post chemotherapy complications related to HCV infection.

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Fluctuation of serum HCV-RNA levels in untreated blood donors with chronic hepatitis C virus infection

Journal of Viral Hepatitis ◽

10.1046/j.1365-2893.2002.00333.x ◽

2002 ◽

Vol 9 (1) ◽

pp. 36-42 ◽

Cited By ~ 12

Author(s):

I. K. Kuramoto ◽

T. Moriya ◽

V. Schoening ◽

P. V. Holland

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Virus Infection ◽

Chronic Hepatitis C ◽

Blood Donors ◽

Hcv Rna ◽

Hepatitis C Virus Infection ◽

Chronic Hepatitis C Virus ◽

Rna Levels

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Prevalence of Occult Hepatitis C Virus Infection in Beta-Thalassemia Major Patients in Ahvaz, Iran

10.21203/rs.3.rs-191652/v1 ◽

2021 ◽

Author(s):

sepideh Nasimzadeh ◽

azarakhsh azaran ◽

shahram Jalilian ◽

Manoochehr Makvandi ◽

Seyed Saeid Seyedian ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Virus Infection ◽

Thalassemia Major ◽

Beta Thalassemia ◽

Hcv Rna ◽

Hepatitis C Virus Infection ◽

Occult Hepatitis ◽

Beta Thalassemia Major ◽

Occult Hepatitis C

Abstract Background: Occult hepatitis C virus infection (OCI) is defined by the presence of HCV RNA in peripheral blood mononuclear cells (PBMCs) and liver tissue cells despite the absence of HCV RNA in plasma. Currently, OCI is classified into two types: seropositive OCI (anti-HCV positive and serum HCV-RNA negative) and seronegative OCI (anti-HCV and serum HCV-RNA negative). Beta-Thalassemia is described as a blood disorder, which decreases the synthesis of hemoglobin. Repeated blood transfusion is the standard treatment for patients with beta-thalassemia major (BTM) that increases the risk of exposure to infectious agents. This study aimed to investigate the prevalence of OCI among BTM patients.Materials and Methods: plasma and PBMCs were collected from 90 BTM patients and screened for HCV antibody using the ELISA kit commercially as the first step. Then nested-RT PCR was performed on extractions of plasma and PBMC. Positive samples of HCV RNA from PBMCs were sequenced and aligned to construct the HCV phylogenetic tree to assess the homology of sequences compared to the reference sequences retrieved from GenBank.Results: Seventy-nine out of 90 cases (87.8%) indicated negative results for HCV Ab (seronegative), while 11 patients (12.2%) were seropositive. HCV RNA was found in PBMCs samples of four patients (66.66%) with negative HCV Ab (seronegative) and two patients (33.3%) with positive HCV Ab (seropositive). HCV RNA was not detected in plasma samples of these six patients. Overall six out of 90 patients (6.7%) had OCI. HCV genotyping revealed that all six patients infected with HCV subtype 3a.Conclusion: We indicated the high frequency of OCI in BTM patients. Nevertheless, more attention is warranted, considering the importance of this infection. Also, further studies are necessary to determine the actual prevalence of OCI among BTM patients in Iran.

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The usefulness of Umelosa hepatitis C vírus qualitative kit as supplemental test for confirmation of hepatitis C virus infection

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822004000100007 ◽

2004 ◽

Vol 37 (1) ◽

pp. 25-27

Author(s):

Idania Gonzalez-Perez ◽

Yaimé Josefina González González ◽

Ariel Vina-Rodriguez ◽

Anny Armas Cayarga ◽

Rosa Lydia Solís

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Virus Infection ◽

Untranslated Region ◽

Blood Donors ◽

Pcr Analysis ◽

Hcv Rna ◽

Hepatitis C Virus Infection ◽

Pcr Assay ◽

Blood Banks

Forty voluntary blood donors from two different blood banks in Havana, Cuba, who were repeatedly reactive on the routine screening of antibodies to hepatitis C virus, by Umelisa HCV test, were analyzed for the presence of HCV RNA using a nested PCR assay of the HCV 5' untranslated region, Umelosa HCV qualitative. Sera from 45 patients of a specialized gastroenterology consultation, positive to Umelisa HCV, were also assayed with the Umelosa HCV qualitative, to establish their condition related to the presence of HCV RNA previously to the indication of a treatment or after three, six or twelve months of antiviral therapy. Serum HCV-RNA was detected in 21/40 (52.5%) donors who had repeatedly positive ELISA results, confirming the HCV infection for them. In specialized consultation HCV-RNA was detected by PCR analysis in 30/45 (66%) analyzed sera.

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