Abstract 4834: Microarray gene expression analysis on formalin-fixed, paraffin embedded material is feasible and the resulting profiles are comparable to profiles from fresh frozen matched material

2011 ◽  
Author(s):  
Lorenza Mittempergher ◽  
Jorma J. de Ronde ◽  
Marja Nieuwland ◽  
Ron M. Kerkhoven ◽  
Iris Simon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Microarray Gene Expression ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Fresh Frozen ◽  
Microarray Gene ◽  
Microarray Gene Expression Analysis ◽  
Formalin Fixed

scholarly journals Maximizing RNA Yield from Archival Renal Tumors and Optimizing Gene Expression Analysis

2009 ◽  
Vol 15 (1) ◽  
pp. 80-85 ◽  
Author(s):  
Sean T. Glenn ◽  
Karen L. Head ◽  
Bin T. Teh ◽  
Kenneth W. Gross ◽  
Hyung L. Kim
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Reverse Transcription ◽  
Gene Expression Analysis ◽  
Rna Isolation ◽  
Proteinase K ◽  
Specific Primers ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Formalin-fixed, paraffin-embedded tissues are widely available for gene expression analysis using TaqMan ® PCR. Five methods, including 4 commercial kits, for recovering RNA from paraffin-embedded renal tumor tissue were compared. The MasterPure™ kit from Epicentre produced the highest RNA yield. However, the difference in RNA yield between the kit from Epicenter and Invitrogen’s TRIzol method was not significant. Using the top 3 RNA isolation methods, the manufacturers’ protocols were modified to include an overnight Proteinase K digestion. Overnight protein digestion resulted in a significant increase in RNA yield. To optimize the reverse transcription reaction, conventional reverse transcription with random oligonucleotide primers was compared to reverse transcription using primers specific for genes of interest. Reverse transcription using gene-specific primers significantly increased the quantity of cDNA detectable by TaqMan ® PCR. Therefore, expression profiling of formalin-fixed, paraffin-embedded tissue using TaqMan® qPCR can be optimized by using the MasterPure™ RNA isolation kit modified to include an overnight Proteinase K digestion and gene-specific primers during the reverse transcription.

scholarly journals cDNA Microarray Gene Expression Analysis and Toxicological Phenotype for Anticancer Drug

2004 ◽  
Vol 66 (11) ◽  
pp. 1339-1345 ◽  
Author(s):  
Gyoung-Jae LEE ◽  
Wan-Seon LEE ◽  
Ki-Seon JEON ◽  
Chan-Hwi UM ◽  
Yang-Suk KIM ◽  
...  
Keyword(s):  
Gene Expression ◽  
Anticancer Drug ◽  
Expression Analysis ◽  
Cdna Microarray ◽  
Gene Expression Analysis ◽  
Microarray Gene Expression ◽  
Microarray Gene ◽  
Microarray Gene Expression Analysis

scholarly journals Microarray gene expression analysis of neutrophils from elderly septic patients

Genomics Data ◽  
2015 ◽  
Vol 6 ◽  
pp. 51-53 ◽  
Author(s):  
Diogo Vieira da Silva Pellegrina ◽  
Patricia Severino ◽  
Marcel Cerqueira Machado ◽  
Fabiano Pinheiro da Silva ◽  
Eduardo Moraes Reis
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Microarray Gene Expression ◽  
Microarray Gene ◽  
Microarray Gene Expression Analysis
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