Abstract 261: Long-read sequencing characterization of a patient with bilateral Wilms tumor of unknown etiology

There have been many reports of the association between hemihypertrophy and intraabdominal masses, including Wilms' tumor, hepatoma, and adrenal cortical neoplasias.1 The present report describes a patient with segmental hemihypertrophy, multiple ovarian cysts, and bilateral Wilms' tumor and suggests a screening regimen for patients with hemihypertrophy. CASE REPORT The patient was a 9 lb 14 oz product of a term pregnancy, who was well until a mass filling the entire right side of the abdomen was discovered on routine examination at 4 months of age. Intravenous pyelogram (IVP) confirmed a large prerenal mass which on ultrasound was felt to be an ovarian cyst.

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Dual Isoform Sequencing Reveals a Multifaceted Transcriptional Architecture of a Prototype Baculovirus

10.21203/rs.3.rs-637036/v1 ◽

2021 ◽

Author(s):

Gábor Torma ◽

Dóra Tombácz ◽

Norbert Moldován ◽

Ádám Fülöp ◽

István Prazsák ◽

...

Keyword(s):

Protein Coding ◽

Rna Molecules ◽

Non Coding Rna ◽

Oxford Nanopore ◽

The Pacific ◽

Viral Genes ◽

Long Read ◽

Oxford Nanopore Technologies ◽

Overlapping Transcripts

Abstract In this study, we used two long-read sequencing (LRS) techniques, Sequel from the Pacific Biosciences and MinION from Oxford Nanopore Technologies, for the transcriptional characterization of a prototype baculovirus, Autographacalifornica multiple nucleopolyhedrovirus. LRS is able to read full-length RNA molecules, and thereby to distinguish between transcript isoforms, mono- and polycistronic RNAs, and overlapping transcripts. Altogether, we detected 875 transcripts, of which 759 are novel and 116 have been annotated previously. These RNA molecules include 41 novel putative protein coding transcript (each containing 5’-truncated in-frame ORFs), 14 monocistronic transcripts, 99 multicistronic RNAs, 101 non-coding RNA, and 504 length isoforms. We also detected RNA methylation in 12 viral genes and RNA hyper-editing in the longer 5’-UTR transcript isoform of ORF 19 gene.

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Nodular regenerative hyperplasia of the liver simulating metastases following treatment for bilateral Wilms tumor

Medical and Pediatric Oncology ◽

10.1002/mpo.10038 ◽

2003 ◽

Vol 41 (1) ◽

pp. 85-87 ◽

Cited By ~ 10

Author(s):

Winnie C.W. Chu ◽

Derek J. Roebuck

Keyword(s):

Wilms Tumor ◽

Nodular Regenerative Hyperplasia ◽

Bilateral Wilms Tumor

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First Report of a Foodborne Salmonella enterica Serovar Gloucester (4:i:l,w) ST34 Strain Harboring blaCTX–M–55 and qnrS Genes Located in IS26-Mediated Composite Transposon

Frontiers in Microbiology ◽

10.3389/fmicb.2021.646101 ◽

2021 ◽

Vol 12 ◽

Author(s):

Lili Li ◽

Rikke Heidemann Olsen ◽

Anhua Song ◽

Jian Xiao ◽

Chong Wang ◽

...

Keyword(s):

Salmonella Enterica ◽

Structural Unit ◽

Bacterial Species ◽

Meat Products ◽

Sequence Type ◽

E Coli ◽

Serovar Typhimurium ◽

Long Read ◽

Phenotypic And Genotypic Characterization

Extended-spectrum β-lactamases (ESBLs) production and (fluoro)quinolone (FQ) resistance among Salmonella pose a public health threat. The objective of this study was the phenotypic and genotypic characterization of an ESBL-producing and nalidixic acid-resistant Salmonella enterica serovar Gloucester isolate (serotype 4:i:l,w) of sequence type 34 (ST34) from ready-to-eat (RTE) meat products in China. Whole-genome short and long read sequencing (HiSeq and MinION) results showed that it contained blaCTX–M–55, qnrS1, and tetB genes, with blaCTX–M–55 and qnrS1 located in chromosomal IS26-mediated composite transposon (IS26–qnrS1–IS3–Tn3–orf–blaCTX–M–55–ISEcp1–IS26). The same genetic structure was found in the chromosome of S. enterica subsp. enterica serovar Typhimurium strain and in several plasmids of Escherichia coli, indicating that the IS26-mediated composite transposon in the chromosome of S. Gloucester may originate from plasmids of E. coli and possess the ability to disseminate to Salmonella and other bacterial species. Besides, the structural unit qnrS1–IS3–Tn3–orf–blaCTX–M–55 was also observed to be linked with ISKpn19 in both the chromosomes and plasmids of various bacteria species, highlighting the contribution of the insertion sequences (IS26 and ISKpn19) to the co-dissemination of blaCTX–M–55 and qnrS1. To our knowledge, this is the first description of chromosomal blaCTX–M–55 and qnrS in S. Gloucester from RTE meat products. Our work expands the host range and provides additional evidence of the co-transfer of blaCTX–M–55 and qnrS1 among different species of Salmonella through the food chain.

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