Replication banding and FISH analysis reveal the origin of the Hyla femoralis karyotype and XY/XX sex chromosomes

2003 ◽  
Vol 101 (1) ◽  
pp. 80-83 ◽  
Author(s):  
J.E. Wiley
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Replication Banding ◽  
Hyla Femoralis

The conservation of number and location of 18S sites indicates the relative stability of rDNA in species of Pentatomomorpha (Heteroptera)

Genome ◽  
2013 ◽  
Vol 56 (7) ◽  
pp. 425-429 ◽  
Author(s):  
Vanessa Bellini Bardella ◽  
Thiago Fernandes ◽  
André Luís Laforga Vanzela
Keyword(s):  
Sex Chromosomes ◽  
18S Rdna ◽  
Fluorescent In Situ Hybridization ◽  
Evolutionary History ◽  
Conclusive Evidence ◽  
Fish Analysis ◽  
Comparative Cytogenetics ◽  
Rdna Sites ◽  
History Of

Fluorescent in situ hybridization (FISH) with rDNA probes has been used for comparative cytogenetics studies in different groups of organisms. Although heteropterans are a large suborder within Hemiptera, studies using rDNA are limited to the infraorder Cimicomorpha, in which rDNA sites are present in the autosomes or sex chromosomes. We isolated and sequenced a conserved 18S rDNA region of Antiteuchus tripterus (Pentatomidae) and used it as a probe against chromosomes of 25 species belonging to five different families of Pentatomomorpha. The clone pAt05, with a length of 736 bp, exhibited a conserved stretch of 590 bp. FISH analysis with the probe pAt05 always demonstrated hybridization signals in sub-terminal positions, except for Euschistus heros. Apparently, there is a tendency for 18S rDNA sites to locate in autosomes, except for Leptoglossus gonagra and Euryophthalmus rufipennis, which showed signals in the m- and sex chromosomes, respectively. Although FISH has produced evidence that rearrangements are involved in rDNA repositioning, whether in different autosomes or between sex and m-chromosomes, we have no conclusive evidence of what were the pathways of these rearrangements based on the evolutionary history of the species studied here. Nevertheless, the diversity in the number of species analyzed here showed a tendency of 18S rDNA to remain among the autosomes.

Segregation of sex chromosomes into sperm nuclei in a man with 47,XXY Klinefelter's karyotype: a FISH analysis

1997 ◽  
Vol 99 (4) ◽  
pp. 474-477 ◽  
Author(s):  
M. Guttenbach ◽  
H. W. Michelmann ◽  
B. Hinney ◽  
W. Engel ◽  
M. Schmid
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Sperm Nuclei

PCR-PRINS-FISH analysis of structurally abnormal sex chromosomes in eight patients with Turner phenotype

2001 ◽  
Vol 60 (5) ◽  
pp. 385-392 ◽  
Author(s):  
A Cervantes ◽  
R Guevara-Yáñez ◽  
M López ◽  
N Monroy ◽  
M Aguinaga ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Turner Phenotype

Early replication banding in Leporinus species (Osteichthyes, Characiformes) bearing differentiated sex chromosomes (ZW)

Genetica ◽  
2006 ◽  
Vol 130 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Wagner Franco Molina ◽  
Pedro Manoel Galetti
Keyword(s):  
Sex Chromosomes ◽  
Replication Banding ◽  
Early Replication

scholarly journals A Turner syndrome case associated with dic(Y;22)

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Rie Kawamura ◽  
Hidehito Inagaki ◽  
Midori Yamada ◽  
Fumihiko Suzuki ◽  
Yuki Naru ◽  
...  
Keyword(s):  
Turner Syndrome ◽  
Loss Of Heterozygosity ◽  
Sex Chromosomes ◽  
Chromosome 22 ◽  
Fish Analysis ◽  
Mosaic Pattern ◽  
Case Presentation ◽  
Snp Microarray ◽  
Female Case ◽  
Pseudoautosomal Regions

Abstract Background Constitutional telomeric associations are very rare events and the mechanism underlying their development is not well understood. Case presentation We here describe a female case of Turner syndrome with a 45,X,add(22)(p11.2)[25]/45,X[5]. We reconfirmed this karyotype by FISH analysis as 45,X,dic(Y;22)(p11.3;p11.2)[28]/45,X[2].ish dic(Y;22)(SRY+,DYZ1+). A possible mechanism underlying this mosaicism was a loss of dic(Y;22) followed by a monosomy rescue of chromosome 22. However, SNP microarray analysis revealed no loss of heterozygosity (LOH) in chromosome 22, although a mosaic pattern of LOH was clearly detectable at the pseudoautosomal regions of the sex chromosomes. Conclusions Our results suggest that the separation of the dicentric chromosome at the junction resulted in a loss of chromosome Y without a loss of chromosome 22, leading to this patient’s unique mosaicism. Although telomere signals were not detected by FISH at the junction, it is likely that the original dic(Y;22) chromosome was generated by unstable telomeric associations. We propose a novel “pulled apart” mechanism as the process underlying this mosaicism.

Duck-billed platypus boasts ten sex chromosomes

Nature ◽  
2004 ◽  
Author(s):  
Roxanne Khamsi
Keyword(s):  
Sex Chromosomes

Immunophenotyping and cytogenetic profile of children with acute lymphoblastic leukemia: A study from a tertiary care center from Kashmir.

JMS SKIMS ◽  
2020 ◽  
Vol 23 (2) ◽  
Author(s):  
Faisal R Guru ◽  
Nisar Ahmad Syed ◽  
Shumail Bashir ◽  
Sanudev Sadanandan Vp ◽  
Hashim Kunju Ismail ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Cytogenetic Study ◽  
Lymphoblastic Leukemia ◽  
Tertiary Care ◽  
Medical Science ◽  
Fish Analysis ◽  
Jammu And Kashmir ◽  
Mll Gene ◽  
Cytogenetic Profile ◽  
Study Participants

Background The complete cytogenetic and immunophenotyping data in children suffering from acute lymphoblastic leukemia (ALL) in Jammu and Kashmir is scarce. To bridge this knowledge gap the present study proposes to evaluate the immunophenotype and cytogenetic profile of pediatric ALL patients treated in our hospital. Material and methods This hospital-based observational study was conducted on 180 pediatric patients aged between 1  to 18 years who had visited the Paediatric unit of the  Department of Medical Oncology at Sher-I -Kashmir Institute of Medical Science, Srinagar ,Jammu and Kashmir between the January 2015 to December 2019. Result Among the study participants, 57.8% were male and 42.2% were female with a mean age of 9.24 years and median of 8 Years. Among the participants, 57.2% were below 10 years of age and 42.8% were above 10years of age. CNS disease was reported in 7.8%  of the study participants.  63.3% patients  had a TLC count of less than 20000. Immunophenotyping data revealed pre-B ALL in 77.8% of children. Cytogenetic study was conducted on 153 patients among them 74.4% had a normal karyotype, 7.2% s had hyperdiploidy and 3.3% had hypodiploidy. The FISH analysis showed that 23.3% of study participants were positive for the TEL-AML study, 11.1% were positive for BCR-ABL analysis and 4.4% of participants were positive for MLL gene analysis. The overall survival in the study population was 78.9% among the study participants. Only the MLL gene rearrangement analysis showed a statistically significant correlation with the survival analysis (P<0.5). Conclusion In summary, the present study reported the complete cytogenetic and immunophenotyping profile of the children suffering from acute lymphoblastic leukemia in Jammu and Kashmir.

Application of bovine heterosome painting probes to identification sex chromosomes in goral (Nemorhaedus caudatus) / Wykorzystanie bydlęcych sond malujących heterosomy do identyfikacji chromosomów płci u gorala (Nemorhaedus caudatus)

2013 ◽  
Vol 30 (4) ◽  
Author(s):  
Anna Kozubska-Sobocińska ◽  
Barbara Danielak-Czech ◽  
Marek Babicz ◽  
Barbara Rejduch ◽  
Agnieszka Bąk
Keyword(s):  
Sex Chromosomes ◽  
Painting Probes
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