Steroid Signaling System Responds Differently to Temperature and Hormone Manipulation in the Red-Eared Slider Turtle (Trachemys scripta elegans), a Reptile with Temperature-Dependent Sex Determination

2007 ◽  
Vol 1 (3) ◽  
pp. 181-196 ◽  
Author(s):  
M. Ramsey ◽  
D. Crews
Keyword(s):  
Sex Determination ◽  
Trachemys Scripta ◽  
Signaling System ◽  
Temperature Dependent ◽  
Trachemys Scripta Elegans ◽  
Slider Turtle ◽  
Steroid Signaling

Temperature-dependent sex determination is mediated by pSTAT3 repression of Kdm6b

Science ◽  
2020 ◽  
Vol 368 (6488) ◽  
pp. 303-306 ◽  
Author(s):  
Ceri Weber ◽  
Yingjie Zhou ◽  
Jong Gwan Lee ◽  
Loren L. Looger ◽  
Guoying Qian ◽  
...  
Keyword(s):  
Causal Link ◽  
Trachemys Scripta ◽  
Temperature Sensitive ◽  
Signal Transducer ◽  
Temperature Dependent ◽  
Trachemys Scripta Elegans ◽  
Slider Turtle ◽  
Stat3 Phosphorylation ◽  
Epigenetic Regulator ◽  
Transcription 3

In many reptiles, including the red-eared slider turtle Trachemys scripta elegans (T. scripta), sex is determined by ambient temperature during embryogenesis. We previously showed that the epigenetic regulator Kdm6b is elevated at the male-producing temperature and essential to activate the male pathway. In this work, we established a causal link between temperature and transcriptional regulation of Kdm6b. We show that signal transducer and activator of transcription 3 (STAT3) is phosphorylated at the warmer, female-producing temperature, binds the Kdm6b locus, and represses Kdm6b transcription, blocking the male pathway. Influx of Ca2+, a mediator of STAT3 phosphorylation, is elevated at the female temperature and acts as a temperature-sensitive regulator of STAT3 activation.

scholarly journals An Annotated Chromosome-Level Reference Genome of the Red-Eared Slider Turtle (Trachemys scripta elegans)

2020 ◽  
Vol 12 (4) ◽  
pp. 456-462 ◽  
Author(s):  
Warren Brian Simison ◽  
James F Parham ◽  
Theodore J Papenfuss ◽  
Athena W Lam ◽  
James B Henderson
Keyword(s):  
Reference Genome ◽  
Single Copy ◽  
Trachemys Scripta ◽  
Future Research ◽  
Chromosome Counts ◽  
Trachemys Scripta Elegans ◽  
Chromosome Fusion ◽  
Slider Turtle ◽  
Tetrapod Evolution ◽  
Chromosome Level

Abstract Among vertebrates, turtles have many unique characteristics providing biologists with opportunities to study novel evolutionary innovations and processes. We present here a high-quality, partially phased, and chromosome-level Red-Eared Slider (Trachemys scripta elegans, TSE) genome as a reference for future research on turtle and tetrapod evolution. This TSE assembly is 2.269 Gb in length, has one of the highest scaffold N50 and N90 values of any published turtle genome to date (N50 = 129.68 Mb and N90 = 19 Mb), and has a total of 28,415 annotated genes. We introduce synteny analyses using BUSCO single-copy orthologs, which reveal two chromosome fusion events accounting for differences in chromosome counts between emydids and other cryptodire turtles and reveal many fission/fusion events for birds, crocodiles, and snakes relative to TSE. This annotated chromosome-level genome will provide an important reference genome for future studies on turtle, vertebrate, and chromosome evolution.

scholarly journals Genetic network underlying temperature-dependent sex determination is endogenously regulated by temperature in isolated cultured Trachemys scripta gonads

2010 ◽  
Vol 239 (4) ◽  
pp. 1061-1075 ◽  
Author(s):  
Christina M. Shoemaker-Daly ◽  
Kyle Jackson ◽  
Ryohei Yatsu ◽  
Yuiko Matsumoto ◽  
David Crews
Keyword(s):  
Sex Determination ◽  
Genetic Network ◽  
Trachemys Scripta ◽  
Temperature Dependent
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