A Recombinant DNA Plasmid Encoding the Human Interleukin-5 Breaks Immunological Tolerance and Inhibits Airway Inflammation in a Murine Model of Asthma

2007 ◽  
Vol 145 (4) ◽  
pp. 313-323 ◽  
Author(s):  
Guang-Hong Tan ◽  
Jing-Mei Su ◽  
Cai-Chun Wang ◽  
Feng-Ying Huang ◽  
Hua Wang ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Murine Model ◽  
Recombinant Dna ◽  
Immunological Tolerance ◽  
Interleukin 5 ◽  
Dna Plasmid

scholarly journals Specific Immunotherapy in a Murine Model of Grass Pollen (Phl p5b)-Induced Airway Inflammation

2021 ◽  
Vol 2 ◽  
Author(s):  
Matthias Stiehm ◽  
Marcus Peters
Keyword(s):  
Airway Inflammation ◽  
Murine Model ◽  
Grass Pollen ◽  
Specific Immunotherapy ◽  
Allergen Challenge ◽  
Allergic Airway Inflammation ◽  
Interleukin 5 ◽  
Grass Pollen Allergen ◽  
Adjuvant Effects

Background: The use of ovalbumin as a model allergen in murine models of allergic asthma is controversially discussed since it is not an aeroallergen and sensitization can only be achieved by using strong Th2-inducing adjuvants. Therefore, in this study, a murine model of asthma has been established in which sensitization against the major grass pollen allergen Phl p5b was performed without using aluminum hydroxide (alum). We used this model for specific immunotherapy.Methods: Female, 5–6-week-old mice were sensitized by six subcutaneous (s.c.) injections of 20 μg Phl p5b followed by four provocations to induce allergic airway inflammation. For desensitization, 1 mg of Phl p5b was injected subcutaneously during allergen challenge for one to a maximum of four times. Three days after the last challenge, the allergic immune response was analyzed.Results: Sensitized and challenged animals showed a significant infiltration of eosinophils into the airways, and the production of interleukin-5 (IL-5) by in vitro re-stimulated splenocytes could be detected. Furthermore, hyper-responsiveness of the airways was verified by invasive measurement of airway resistance in methacholine-challenged animals. Desensitized animals showed a significant reduction of all parameters.Conclusion: In this study, a murine model of asthma has successfully been established by sensitization against the clinically relevant allergen Phl p5b without using alum. S.c. injection of allergen dose dependently led to desensitization of sensitized mice. We suggest that this model is useful to study adjuvant effects of immune modulatory substances on immunotherapy without the interference of alum.

A Recombinant DNA Plasmid Encoding the sIL-4R-NAP Fusion Protein Suppress Airway Inflammation in an OVA-Induced Mouse Model of Asthma

Inflammation ◽  
2016 ◽  
Vol 39 (4) ◽  
pp. 1434-1440 ◽  
Author(s):  
Xin Liu ◽  
Guo Fu ◽  
Zhenyu Ji ◽  
Xiabing Huang ◽  
Cong Ding ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fusion Protein ◽  
Airway Inflammation ◽  
Recombinant Dna ◽  
Dna Plasmid

DZNep attenuates allergic airway inflammation in an ovalbumin-induced murine model

2020 ◽  
Author(s):  
Hang Li ◽  
Jian Li ◽  
Tong Lu ◽  
Dehua Chen ◽  
Rui Xu ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Murine Model ◽  
Allergic Airway Inflammation

L -Selectin is required for the development of airway hyperresponsiveness but not airway inflammation in a murine model of asthma

2001 ◽  
Vol 107 (6) ◽  
pp. 1019-1024 ◽  
Author(s):  
Lynne C. Fiscus ◽  
Jenny Van Herpen ◽  
Douglas A. Steeber ◽  
Thomas F. Tedder ◽  
Mimi L.K. Tang
Keyword(s):  
Airway Inflammation ◽  
Murine Model ◽  
Airway Hyperresponsiveness

scholarly journals The triterpenoid lupeol attenuates allergic airway inflammation in a murine model

2008 ◽  
Vol 8 (9) ◽  
pp. 1216-1221 ◽  
Author(s):  
J.F. Vasconcelos ◽  
M.M. Teixeira ◽  
J.M. Barbosa-Filho ◽  
A.S.S.C. Lúcio ◽  
J.R.G.S. Almeida ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Murine Model ◽  
Allergic Airway Inflammation

Caffeic acid phenethyl ester attenuates allergic airway inflammation and hyperresponsiveness in murine model of ovalbumin-induced asthma

Life Sciences ◽  
2008 ◽  
Vol 82 (13-14) ◽  
pp. 797-805 ◽  
Author(s):  
Won-Kyo Jung ◽  
Da-Young Lee ◽  
Yung Hyun Choi ◽  
Sung Su Yea ◽  
Inhak Choi ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Caffeic Acid ◽  
Murine Model ◽  
Allergic Airway Inflammation ◽  
Caffeic Acid Phenethyl Ester
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