In birch roots (Betula pendula Roth), two members of the
Bet v 1 gene family which encode PR 10 proteins have
previously been characterized. One of these members, named
Bet v 1-sc1, is significantly induced in response to
biotic or abiotic factors. We have analysed the expression of
Bet v 1-sc1 in birch roots treated either with 1 M
indole-3-acetic acid (IAA) or 1 M kinetin using reverse
transcription–polymerase chain reaction (RT–PCR), northern
blotting and competitive PCR. High accumulation of the
Bet v 1-sc1 transcripts was recorded only after auxin
application, while kinetin had no effect. By in situ
hybridization, we have investigated the localization of
Bet v 1-sc1 mRNA in birch roots after induction of the
gene by root treatment with 1 M IAA. Using root tip sections, we showed that
Bet v 1-sc1 is significantly expressed in the apical
meristem and the procambium. In sections taken in the zone producing lateral
roots, the presence of Bet v 1-sc1 was found at sites of
emerging secondary root primordia. This first report of localization of
Bet v 1-sc1 expression suggests that this gene could be
involved in the processes leading to lateral root initiation.
Localization of the human gene encoding the 13.3-kDa subunit of mitochondrial complex (UQCRB) to 8q22 by in situ hybridization
