The succinic dehydrogenase and lipid content of follicular and luteal cells of the bovine ovary

1968 ◽  
Vol 71 (4) ◽  
pp. 542-564 ◽  
Author(s):  
Janis Priedkalns ◽  
Alvin F. Weber
1977 ◽  
Vol 55 (6) ◽  
pp. 617-624 ◽  
Author(s):  
J. M. Molnar ◽  
E. V. Parups

The starch, lipid, cytochrome oxidase (EC 1.9.3.1), succinic dehydrogenase (EC 1.3.99.1), peroxidase (EC 1.11.1.7), and acid phosphatase (EC 3.1.3.2) levels were determined periodically by histochemical methods in transverse sections of cut stem of the rose, Rosa hybrida L. cv. Forever Yours, kept in water or in an aqueous preservative solution containing 4% sucrose, 100 ppm sodium isoascorbate, and 100 ppm 8-hydroxyquinoline sulfate. Senescence of the cutrose stem, including leaves and flowers, was delayed by use of the sugar solution. The levels of cytochrome oxidase and succinic dehydrogenase were not significantly affected by either of the treatments. Starch, lipid, peroxidase, and acid phosphatase levels decreased in the tissues of rapidly senescing stems kept in water. In tissues where senescence was retarded by use of sugar solutions the lipid content and peroxidase were maintained at a relatively high level; starch, and acid phosphatase levels steadily increased. It is suggested that in cut rose stems, the onset or retardation of senescence is not related to the activities of acid phosphatase or peroxidase.


1968 ◽  
Vol 59 (2_Suppl) ◽  
pp. S7-S33 ◽  
Author(s):  
B. L. Lobel ◽  
E. Levy

ABSTRACT Activities of several hydrolytic enzymes (acid and alkaline phosphatases, ATP-ase and β-glucuronidase) as well as several dehydrogenases (succinic dehydrogenase, NADP and NADPH2 diaphorases, Δ5-3β-, 17β- and 20β-hydroxysteroid dehydrogenases) were studied during the growth and maturation of follicles from primordial to pre-ovulatory in the bovine ovary. Follicular growth apparently commenced with enlargement of the oocyte, and enzymic activities (alkaline phosphatase, ATP-ase, β-glucuronidase and the diaphorases) of this stage were strong in the granulosa; vesicular follicles exhibited strong enzymic activities mainly in the theca. Steroid dehydrogenases were demonstrable in both theca and granulosa of vesicular follicles only. Luteinization of pre-ovulatory follicles, as judged by cytochemical and morphological characteristics, began prior to rupture. A correlation was observed between cytochemically demonstrable enzymic activities and secretory function.


2001 ◽  
Vol 24 (6) ◽  
pp. 327-334 ◽  
Author(s):  
A. Force ◽  
G. Grizard ◽  
M. N. Giraud ◽  
C. Motta ◽  
B. Sion ◽  
...  

2001 ◽  
Vol 120 (5) ◽  
pp. A466-A466
Author(s):  
H KUSUNOKI ◽  
K HARUMA ◽  
J HATA ◽  
K FUTAGAMI ◽  
M HARA ◽  
...  
Keyword(s):  

2002 ◽  
Vol 46 (2) ◽  
pp. 127
Author(s):  
Sun Jin Hur ◽  
Seok Hwan Shin ◽  
Geum Nan Jee ◽  
Eun Joo Yun ◽  
Soon Gu Cho ◽  
...  

1967 ◽  
Vol 56 (3_Suppla) ◽  
pp. S7-S45 ◽  

ABSTRACT Autoradiographic, enzymic and histologic studies on uteri of pregnant rats were carried out to follow the endometrial modifications which take place during progestation (days L0 – L4) and culminate in the state of uterine receptivity essential for ovum-implantation. Pulse labelling with tritiated thymidine (radioactive DNA precursor) on L0, L1 and L2 revealed a sequence of cell renewal in luminal and glandular epithelium and endometrial stroma. On L3 and L4 stromal cells showed extensive incorporation of tritiated thymidine. This synthetic activity was associated with endometrial preparation for decidualization and was evoked at least in part, by the surge of oestrogen on L3. All layers of the uterine wall were heavily infiltrated on L0 and resembled the site of an acute inflammatory reaction. Subsequently, polymorphonuclear infiltration diminished and monocytic cells predominated. On L3 a spatial arrangement was observed: eosinophiles were concentrated in the basal endometrium and monocytic cells in the subepithelial stroma. A comparison was made between such a shift in migratory cells in the uterus and similar phenomena which occur in inflammatory and immune reactions. Activities of acid and alkaline phosphatases, of ATP-ase and succinic dehydrogenase were low on L0 and L1 during the periods of infiltration, degeneration and regeneration of luminal and glandular epithelium. Enzymic activities increased on the following days, (L3 and L4). Vascular dilation and engorgement and endometrial oedema were observed near the blastocysts on L4. Most blastocysts incorporated tritiated thymidine after 14.00 h on L4, but some showed uptake before loss of the zona which occurs usually between 14.00 and 16.00 h; therefore, it was assumed that the permeability of the zona increases prior to being shed. Activities of succinic dehydrogenase and acid and alkaline phosphatases were demonstrable in blastocysts on L4 while they were still »free« in the uterine lumen.


1963 ◽  
Vol 42 (4) ◽  
pp. 480-484 ◽  
Author(s):  
B. Eckstein ◽  
R. Landsberg

ABSTRACT The succinic, malic and isocitric dehydrogenases in the ovary of immature and mature, normal and serum gonadotrophin injected rats were examined. The Qo2 of these enzymes were markedly enhanced in the gonadotrophin injected rats of both age groups, except in the case of succinic dehydrogenase in the ovary of the immature rats, where a slight non-significant decrease was noted. It is concluded that in the mature rat ovary, gonadotrophin administration stimulates the activity of all the examined dehydrogenases of the citric acid cycle, whereas in the immature rat ovary, at least the isocitric- and malic dehydrogenases are thus stimulated.


1954 ◽  
Vol 16 (4) ◽  
pp. 315-322 ◽  
Author(s):  
Antti Telkkä ◽  
A. N Kuusisto ◽  
Kimmo K. Mustakallio

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