Експериментальна оцінка процесів регенерації кісткової тканини шляхом аналізу ферментативної активності кислої та лужної фосфатаз

We explored the dynamics of changes activity indexes of acid and alkaline phosphatases on the models of created bone defect and compared the results with the data obtained after leading bone defects plastic with the material based on hydroxyapatite and polilaktide with different percentage of components (the first and the Second research groups). We established that the demineralization process lasts up to 7th days in all groups. At a control already on 14th day it starts to dominate the reverse processes that reach their maximum at 30th day, followed by a balance of both these antagonistic processes. In the first experimental group mineralization processes are sharply intensive in between 14th and 30ty day, and keeping a positive balance even at the 90th and 180th day. In the second experimental group the processes of mineralization is implementing till the 21st day with the highest efficiency. Then, at the 30th day mineralization intensity decreases rapidly and almost completely normalizes on the 180th day.

Effect of heating oil on the activity of soil enzymes and the yield of yellow lupine

The aim of the study was to determine the response of soil enzymes such as dehydrogenases, urease and acid and alkaline phosphatases to heating oil contaminating (0.0, 0.25, 0.5, 0.75, 1.0, 1.5% of soil) the experimental soil supplemented with lime and used for cultivation of yellow lupine of the Markiz variety. An increasing contamination of soil with heating oil stimulated the activity of dehydrogenases and acid and alkaline phosphatases but had a toxic effect on yellow lupine. Lime supplements did not have a significant effect on an average activity of soil dehydrogenases. However, such soil treatment had a significant effect on urease. Increasing heating oil doses in lime-supplemented soil stimulated urease activity, whereas in lime-free soil urease activity was inhibited. The activity of acid and alkaline phosphatase was lower in limed soil than in lime-free soil. The activity of dehydrogenases, urease and alkaline phosphatase in the soil with lupine cultivation was significantly higher than in the unsown soil.