scholarly journals Application of Hanging Drop Technique for Kidney Tissue Culture

2017 ◽  
Vol 42 (2) ◽  
pp. 220-231 ◽  
Author(s):  
Shaohui Wang ◽  
Ximing Wang ◽  
Jasmine Boone ◽  
Jin Wie ◽  
Kay-Pong Yip ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Hanging Drop ◽  
Drop Technique

scholarly journals In Vitro Production of Erythropoietin by Kidneys Perfused With a Serum-free Solution

Blood ◽  
1974 ◽  
Vol 44 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Allan J. Erslev
Keyword(s):  
Tissue Culture ◽  
Atmospheric Pressure ◽  
Culture Medium ◽  
Kidney Tissue ◽  
In Vitro Production ◽  
In Vitro Perfusion ◽  
Serum Free ◽  
Enzymatic Activation ◽  
Vitro Production

Abstract Normal rabbits exposed to 0.4 atmospheric pressure for 3 hr will generate about 40-60 U of erythropoietin during a subsequent 3-hr period. If the kidneys were removed from 3-hr hypoxic animals, washed carefully, and perfused for 3 hr by recirculation with a serum-tissue culture mixture, each kidney generated about 14 U of erythropoietin in vitro. Perfusion of normal kidneys did not result in the production of erythropoietin, and only small amounts were generated if the perfusate contained Puromycin. Three-hour hypoxic kidneys perfused for 3 hr with a serum-free tissue culture medium were found to generate about 8 U of erythropoietin per kidney and similar kidneys perfused with saline about 1 U. These results indicate that erythropoietin is synthesized by kidney tissue and not produced by enzymatic activation of a plasma substrate.

Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail

1999 ◽  
Vol 55 (11) ◽  
pp. 1903-1905 ◽  
Author(s):  
John N. Lisgarten ◽  
James E. Pitts ◽  
Rex A. Palmer ◽  
Colin D. Reynolds ◽  
Minh Hoa Dao-Thi ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Diffraction Pattern ◽  
Helix Pomatia ◽  
Unit Cell ◽  
Hanging Drop ◽  
Helix Pomatia Agglutinin ◽  
Cell Dimensions ◽  
Protein Molecules ◽  
Unit Cell Dimensions ◽  
Drop Technique

Crystals of Helix pomatia agglutinin (HPA) have been grown by the hanging-drop technique using polyethylene glycol as the precipitant at 293 K. Over a period of one to two weeks the crystals grew to maximum dimensions of 0.10 × 0.05 × 0.02 mm. The crystals belong to space group P6322, with unit-cell dimensions a = b = 63.3, c = 105.2 Å and Z = 12 identical monomers of Mr = 13 kDa, aggregating into two 78 kDa hexameric protein molecules per unit cell, each with symmetry 32 (D 3). The diffraction pattern extends to 3.6 Å at 293 K.

Formation of poliovirus in monkey kidney tissue culture cells

Virology ◽  
1962 ◽  
Vol 16 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Heather Donald Mayor ◽  
Liane E. Jordan
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Culture Cells ◽  
Tissue Culture Cells

Cultivation of Enteroviruses in Hamster Kidney Tissue Culture

1959 ◽  
Vol 100 (2) ◽  
pp. 316-320 ◽  
Author(s):  
A. L. Barron ◽  
D. T. Karzon
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue

Morphology of Echo 4 virus grown in monkey kidney tissue culture

Virology ◽  
1962 ◽  
Vol 16 (3) ◽  
pp. 350-353 ◽  
Author(s):  
Philip E. Duffy ◽  
Allen Bell ◽  
Max G. Menefee
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Monkey Kidney
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