An optimized protocol for handling and processing fragile acini cultured with the hanging drop technique

2011 ◽  
Vol 419 (2) ◽  
pp. 348-350 ◽  
Author(s):  
Celia Snyman ◽  
Edith Elliott
Keyword(s):  
Hanging Drop ◽  
Optimized Protocol ◽  
Drop Technique

Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail

1999 ◽  
Vol 55 (11) ◽  
pp. 1903-1905 ◽  
Author(s):  
John N. Lisgarten ◽  
James E. Pitts ◽  
Rex A. Palmer ◽  
Colin D. Reynolds ◽  
Minh Hoa Dao-Thi ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Diffraction Pattern ◽  
Helix Pomatia ◽  
Unit Cell ◽  
Hanging Drop ◽  
Helix Pomatia Agglutinin ◽  
Cell Dimensions ◽  
Protein Molecules ◽  
Unit Cell Dimensions ◽  
Drop Technique

Crystals of Helix pomatia agglutinin (HPA) have been grown by the hanging-drop technique using polyethylene glycol as the precipitant at 293 K. Over a period of one to two weeks the crystals grew to maximum dimensions of 0.10 × 0.05 × 0.02 mm. The crystals belong to space group P6322, with unit-cell dimensions a = b = 63.3, c = 105.2 Å and Z = 12 identical monomers of Mr = 13 kDa, aggregating into two 78 kDa hexameric protein molecules per unit cell, each with symmetry 32 (D 3). The diffraction pattern extends to 3.6 Å at 293 K.

scholarly journals Application of Hanging Drop Technique for Kidney Tissue Culture

2017 ◽  
Vol 42 (2) ◽  
pp. 220-231 ◽  
Author(s):  
Shaohui Wang ◽  
Ximing Wang ◽  
Jasmine Boone ◽  
Jin Wie ◽  
Kay-Pong Yip ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Kidney Tissue ◽  
Hanging Drop ◽  
Drop Technique

MODIFIED HANGING DROP TECHNIQUE

Science ◽  
1932 ◽  
Vol 76 (1978) ◽  
pp. 496-497
Author(s):  
I. H. Blank
Keyword(s):  
Hanging Drop ◽  
Drop Technique

scholarly journals Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique

2016 ◽  
Vol 22 (4) ◽  
pp. 312-321 ◽  
Author(s):  
Matthew J. Ware ◽  
Kevin Colbert ◽  
Vazrik Keshishian ◽  
Jason Ho ◽  
Stuart J. Corr ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Three Dimensional ◽  
Hanging Drop ◽  
Cell Spheroids ◽  
Drop Technique

Crystallization of Pleurotus ostreatus (oyster mushroom) lectin

1999 ◽  
Vol 55 (9) ◽  
pp. 1589-1590 ◽  
Author(s):  
T. K. Chattopadhyay ◽  
J. N. Lisgarten ◽  
R. Brechtel ◽  
H. Rüdiger ◽  
R. A. Palmer
Keyword(s):  
Ammonium Sulfate ◽  
Pleurotus Ostreatus ◽  
Unit Cell ◽  
Oyster Mushroom ◽  
Hanging Drop ◽  
Unit Cell Parameters ◽  
Space Group ◽  
Cell Parameters ◽  
Drop Technique ◽  
Mushroom Lectin

Crystals of Pleurotus ostreatus (oyster mushroom) lectin have been grown by the hanging-drop technique using ammonium sulfate as the precipitant at 293 K. Over a period of between two and three weeks, crystals of hexagonal bipyramidal morphology grew to maximum dimensions of 0.2 × 0.2 × 0.5 mm. The crystals belong to space group P6122 or P6522, with unit-cell parameters a = b = 155.9, c = 149.8 Å, V = 3153078 Å3, Z = 12 (assuming 50% solvent), and diffract to 4.1 Å at 293 K.

scholarly journals Unintentional epidural catheter migration to subarachnoid space followed by continuous spinal anaesthesia: a case report

2014 ◽  
Vol 23 (1) ◽  
pp. 37-39
Author(s):  
K Sardar ◽  
AKMN Chowdhury ◽  
MK Rahman
Keyword(s):  
Epidural Catheter ◽  
Spinal Anaesthesia ◽  
Epidural Space ◽  
Test Dose ◽  
Hemodynamic Instability ◽  
Hanging Drop ◽  
Continuous Spinal Anaesthesia ◽  
Catheter Migration ◽  
Severe Hypotension ◽  
Drop Technique

Among the complications of epidural anaesthesia catheter migration is a very rare one. A 45 years old lady was scheduled for repairing of post caesarean incisional hernia. We prefer the hanging drop technique for epidural space identification, and 3 ml air injection to reconfirm the epidural space. After a test dose of 2% lignocaine 2 ml with 10 microgram adrenaline, the catheter was secured with at 3 cm of its length within the epidural space. Immediately after test dose, she complained of lower limb motor lost. On monitor, bradycardia and severe hypotension was shown. Hemodynamic instability was corrected promptly. After proper resuscitation, we aspirate through epidural catheter. CSF was coming freely. We decided to continue with continuous spinal anaesthesia. We assembled a syringe pump. Continuous spinal anaesthesia was maintained with 0.125% bupivacaine @ 3ml/hour DOI: http://dx.doi.org/10.3329/jbsa.v23i1.18158 Journal of BSA, 2010; 23(1): 34-36

Influence of bacterial density and mating on life history traits of Heterorhabditis bacteriophora

Nematology ◽  
2016 ◽  
Vol 18 (8) ◽  
pp. 963-972 ◽  
Author(s):  
Temesgen Addis ◽  
Shiferaw Demissie ◽  
Olaf Strauch ◽  
Ralf-Udo Ehlers
Keyword(s):  
Life History ◽  
Life History Traits ◽  
Insect Pests ◽  
Heterorhabditis Bacteriophora ◽  
The Body ◽  
Bacterial Density ◽  
Photorhabdus Luminescens ◽  
Hanging Drop ◽  
Offspring Production ◽  
Drop Technique

Heterorhabditis bacteriophora, associated with Photorhabdus luminescens, is commonly used against insect pests. Dauer juveniles (DJ) develop into self-fertilising hermaphrodites that lay eggs until juveniles hatch inside the uterus and feed on the body content of the mother (endotokia matricida). The life history traits of H. bacteriophora were studied at 2.5 × 109, 5 × 109, 10 × 109 and 20 × 109 cells ml−1 of P. luminescens at 25°C using a hanging drop technique. The number of offspring produced per hermaphrodite increased from 50 at 2.5 × 109 cells ml−1 to 269 at 20 × 109 cells ml−1 of P. luminescens. The bacterial density did not influence the beginning of endotokia matricida, hermaphrodite death, DJ release from the maternal carcass and the percentage of juveniles obtained through endotokia matricida. Mating of automictic females could not increase offspring production and survival. Endotokia matricida is an obligatory developmental step in H. bacteriophora.

Successful arterial cannulation in neonates: the ‘hanging drop’ technique

2016 ◽  
Vol 26 (4) ◽  
pp. 458-459
Author(s):  
Riddhi Kundu ◽  
Ganga Prasad
Keyword(s):  
Arterial Cannulation ◽  
Hanging Drop ◽  
Drop Technique
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