scholarly journals Improvement of Blood Processing and Safety by Automation and Pathogen Reduction Technology

2021 ◽  
pp. 1-8
Author(s):  
Ana Isabel Pérez Aliaga ◽  
Gorka Labata ◽  
Alfonso Aranda ◽  
Marcia Cardoso ◽  
Fernando Puente ◽  
...  
Keyword(s):  
Processing System ◽  
Buffy Coat ◽  
Quality Data ◽  
Platelet Concentrates ◽  
Hemoglobin Content ◽  
Blood Processing ◽  
Self Sufficiency ◽  
The Mean ◽  
Pathogen Reduction ◽  
Second Period

<b><i>Introduction:</i></b> The objective of the present study was to describe the experience of the Blood and Tissues Bank of Aragon with the Reveos® Automated Blood Processing System and Mirasol® Pathogen Reduction Technology (PRT) System, comparing retrospectively routine quality data obtained in two different observation periods. <b><i>Methods:</i></b> Comparing quality data encompassing 6,525 blood components from the period 2007–2012, when the semi-automated buffy coat method was used in routine, with 6,553 quality data from the period 2014–2019, when the Reveos system and subsequently the Mirasol system were implemented in routine. <b><i>Results:</i></b> Moving from buffy coat to Reveos led to decreased discard rates of whole blood units (1.2 to 0.1%), increased hemoglobin content (48.1 ± 7.6 to 55.4 ± 6.6 g/unit), and hematocrit (58.9 ± 6.5% to 60.0 ± 4.9%) in red blood cell concentrates. Platelet concentrates (PCs) in both periods had similar yields (3.5 ×10<sup>11</sup>). Whereas in the earlier period, PCs resulted from pooling 5 buffy coats, in the second period 25% of PCs were prepared from 4 interim platelet units. The mean level of factor VIII in plasma was significantly higher with Reveos (92.8 vs. 97.3 IU). Mirasol PRT treatment of PCs reduced expiry rates to 1.2% in 2019. One septic transmission was reported with a non-PRT treated PCs, but none with PRT-treated PCs. <b><i>Conclusion:</i></b> Automation contributed to standardization, efficiency, and improvement of blood processing. Released resources enabled the effortless implementation of PRT. The combination of both technologies guaranteed the self-sufficiency and improvement of blood safety.

Pathogen reduction treatment alters the immunomodulatory capacity of buffy coat-derived platelet concentrates

Transfusion ◽  
2013 ◽  
Vol 54 (3) ◽  
pp. 577-584 ◽  
Author(s):  
Yen S. Loh ◽  
Lacey Johnson ◽  
Matthew Kwok ◽  
Denese C. Marks
Keyword(s):  
Buffy Coat ◽  
Platelet Concentrates ◽  
Reduction Treatment ◽  
Immunomodulatory Capacity ◽  
Pathogen Reduction

Pathogen reduction treatment of buffy coat platelet concentrates in additive solution induces proapoptotic signaling

Transfusion ◽  
2012 ◽  
Vol 52 (10) ◽  
pp. 2094-2103 ◽  
Author(s):  
Samantha Reid ◽  
Lacey Johnson ◽  
Narelle Woodland ◽  
Denese C. Marks
Keyword(s):  
Buffy Coat ◽  
Platelet Concentrates ◽  
Reduction Treatment ◽  
Pathogen Reduction ◽  
Additive Solution

Stem Cell Recovery Following Implementation of an Automated Cord Blood Processing System in a High Volume Laboratory.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4919-4919
Author(s):  
David T. Harris ◽  
Ingrid E. Paulson ◽  
Heather Brown ◽  
Joseph Rosenthal
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Processing System ◽  
Post Processing ◽  
Cell Recovery ◽  
Recovery Rates ◽  
Cell Dose ◽  
Blood Processing ◽  
Percent Recovery ◽  
The Mean

Abstract Background: Many cord blood (CB) processing facilities are transitioning to automated systems to standardize methods and decrease human error. The AutoXpress Platform™ (AXP™) is an automated, functionally closed, sterile CB processing system. When placed within the AXP device and centrifuged, the whole blood product is separated into its composite cell populations and the TNC fraction of the CB unit is separated and automatically delivered into a blow-molded freezing bag at a uniform volume of 21 mL. Objective: This study evaluated the use of the AXP system in a directed donor (DD) family cord blood bank, targeted at producing consistently high TNC and MNC recovery rates regardless of broad variability in collection volume. Methods: Cord blood units were collected between 12/5/2006 and 2/24/2007 from 1414 consenting mothers who elected to preserve and bank CB at Cord Blood Registry (CBR). Collection kits were provided at the time of enrollment, and after delivery, CB was collected from the umbilical cord and transported to CBR’s processing facility in Tucson, Arizona. During the study period, all units arriving at the CBR laboratory were allocated to either ficoll or AXP processing based on the volume, age, and the degree of clotting in each unit. Units processed using AXP had a volume of 40–130 mL, an age of less than 48 hours since collection, and a clotting score of 0 to 2+ (based on an internal scale). TNC and MNC counts were measured both pre- and post-processing, using the Sysmex analyzer. Results: The mean age of cord blood units arriving at the laboratory was 23.59 hours, and the mean collection volume was 72.93 mL (±18.10mL). The mean TNC count post-processing was 9.94x108, the TNC percent recovery was 96.19%, and the mean MNC percent recovery was 98.65%. Volume (mL) Mean Post-Processing TNC (x108) TNC Percent Recovery (%) Mean Post-Processing MNC (x108) MNC Percent Recovery (%) Mean 72.93 9.94 96.19 4.05 98.65 Standard Deviation 18.10 4.43 11.87 1.59 9.16 Conclusions: AXP automated cord blood processing provides consistently high TNC and MNC recovery rates, which has important implications for stem cell dose if the sample is used in transplant. Because limited cell dose is frequently cited as an obstacle to CB transplantation, processing results could impact the usability of each unit. Because DD family CB banks process all units, regardless of collection volume, percent recovery becomes particularly important in evaluating the differences between processing centers. The AXP system yields the highest published cell recovery rate to date and can be easily integrated into a CB processing center such that it decreases the labor and time required for processing while maintaining MNC recovery of greater than 98%.

scholarly journals Comparison of Platelet Concentrates Prepared from Platelet Rich Plasma-Platelet Concentrate and Buffy Coat Poor-Platelet Concentrate on Storage Days 1 and 5

2016 ◽  
Vol 35 (2) ◽  
pp. 5
Author(s):  
Darinnat Buathong ◽  
Jarin Buakaew ◽  
Chutarat Nukfon
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Platelet Rich Plasma ◽  
Buffy Coat ◽  
Platelet Concentrate ◽  
P Value ◽  
Platelet Concentrates ◽  
Hypotonic Shock ◽  
Shock Response ◽  
The Mean

Objective: The purpose of this study is to assess the quality of platelet concentrates on storage days 1 and 5 prepared by platelet rich plasma-platelet concentrate (PRP-PC) and buffy coat poor-platelet concentrate (BC-PC) methods comparing to the American Association of Blood Banks (AABB) recommendations.Material and Method: Totally of 120 platelet concentrates (PC) units on storage days 1 and 5 (60 of PRP-PC triple blood bag and 60 of BC-PC quadruple AS-5 blood bag) were separated from whole blood donations at Songklanagarind Hospital. The prepared PC were assessed with 5 parameters such as volume, platelet count, white blood cell count per unit, pH, swirling phenomenon score and hypotonic shock response. The independent t-tests, paired Student’s t-tests and SPSS program were utilized in statistical analysis step.Results: The mean±standard deviation (S.D.) of each parameter were as follow : (1) Volume of PRP-PC and BC-PC met the standard (40-70 ml). (2) All of the platelet concentrates met the standard (≥ 5.5x1010/unit). The mean±S.D.: PRP-PC and BC-PC (day 1) were 6.820±1.480 x1010 and 7.010±1.300 x1010/unit (p-value=0.260), while PRP-PC and BC-PC (day 5) were 6.620±1.160x1010 and 6.720± 1.150x1010/unit (p-value=0.040). (3) The white blood cell in platelet concentrates met the standard (<0.2x1010/unit). The mean±S.D.: PRP-PC and BC-PC (day 1) were 0.030±0.017 x1010and 0.026±0.019x1010/unit (p-value=0.040), while PRP-PC and BC-PC (day 5) were0.033±0.013x1010 and 0.027±0.019x1010/unit (p-value= 0.580). (4) The pH of all units (PRP-PC and BC-PC) met the standard (≥6.2). The mean±S.D.: PRP-PC and BC-PC (day 1) were 7.430±0.330 and 7.750±0.160 (p-value=0.006), while PRP-PC and BC-PC (day 5) were 7.590±0.350 and 7.620±0.280 (p-value=0.710). The swirling phenomenon score and hypotonic shock response were the same as standard AABB and were not statistically difference.Conclusion: The quality of PRP-PC and BC-PC after storing on days 1 and 5 as follow (1) Volume of PRP-PC and BC-PC met the standard. (2) The platelet count per unit of PRP-PC and BC-PC (day 1), PRP-PC and BC-PC (day 5) were not statistically difference. (3) The white blood cell count per unit of PRP-PC and BC-PC (day 1) were statistically difference, while PRP-PC and BC-PC (day 5) were not statistically difference. (4) The pH of PRP-PC and BC-PC (day 1) were statistically difference, while PRP-PC and BC-PC (day 5) were not statistically difference. The swirling phenomenon score and hypotonic shock response of PRP-PC and BC-PC were not statistically difference. Platelet concentrates of both method storing on days 1 and 5 fulfilled the quality guideline of AABB.

scholarly journals Verbal estimation of the magnitude of time, number, and length

2020 ◽  
Author(s):  
R. S. Ogden ◽  
F. R. Simmons ◽  
J. H. Wearden
Keyword(s):  
Processing System ◽  
Visual Displays ◽  
Verbal Responses ◽  
Physical Length ◽  
Estimation Performance ◽  
Verbal Estimation ◽  
The Mean ◽  
The Way

AbstractPerformance similarities on tasks requiring the processing of different domains of magnitude (e.g. time, numerosity, and length) have led to the suggestion that humans possess a common processing system for all domains of magnitude (Bueti and Walsh in Philos Trans R Soc B 364:1831–1840, 2009). In light of this, the current study examined whether Wearden’s (Timing Time Percept 3:223–245, 2015) model of the verbal estimation of duration could be applied to verbal estimates of numerosity and length. Students (n = 23) verbally estimated the duration, number, or physical length of items presented in visual displays. Analysis of the mean verbal estimates indicated the data were typical of that found in other studies. Analysis of the frequency of individual verbal estimates produced suggested that the verbal responses were highly quantized for duration and length: that is, only a small number of estimates were used. Responses were also quantized for number but to a lesser degree. The data were modelled using Wearden’s (2015) account of verbal estimation performance, which simulates quantization effects, and good fits could be obtained providing that stimulus durations were scaled as proportions (0.75, 1.06, and 0.92 for duration, number, and length, respectively) of their real magnitudes. The results suggest that despite previous reports of similarities in the processing of magnitude, there appear to be differences in the way in which the underlying representations of the magnitudes are scaled and then transformed into verbal outputs.

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