scholarly journals Comparison of Platelet Concentrates Prepared from Platelet Rich Plasma-Platelet Concentrate and Buffy Coat Poor-Platelet Concentrate on Storage Days 1 and 5

2016 ◽  
Vol 35 (2) ◽  
pp. 5
Author(s):  
Darinnat Buathong ◽  
Jarin Buakaew ◽  
Chutarat Nukfon
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Platelet Rich Plasma ◽  
Buffy Coat ◽  
Platelet Concentrate ◽  
P Value ◽  
Platelet Concentrates ◽  
Hypotonic Shock ◽  
Shock Response ◽  
The Mean

Objective: The purpose of this study is to assess the quality of platelet concentrates on storage days 1 and 5 prepared by platelet rich plasma-platelet concentrate (PRP-PC) and buffy coat poor-platelet concentrate (BC-PC) methods comparing to the American Association of Blood Banks (AABB) recommendations.Material and Method: Totally of 120 platelet concentrates (PC) units on storage days 1 and 5 (60 of PRP-PC triple blood bag and 60 of BC-PC quadruple AS-5 blood bag) were separated from whole blood donations at Songklanagarind Hospital. The prepared PC were assessed with 5 parameters such as volume, platelet count, white blood cell count per unit, pH, swirling phenomenon score and hypotonic shock response. The independent t-tests, paired Student’s t-tests and SPSS program were utilized in statistical analysis step.Results: The mean±standard deviation (S.D.) of each parameter were as follow : (1) Volume of PRP-PC and BC-PC met the standard (40-70 ml). (2) All of the platelet concentrates met the standard (≥ 5.5x1010/unit). The mean±S.D.: PRP-PC and BC-PC (day 1) were 6.820±1.480 x1010 and 7.010±1.300 x1010/unit (p-value=0.260), while PRP-PC and BC-PC (day 5) were 6.620±1.160x1010 and 6.720± 1.150x1010/unit (p-value=0.040). (3) The white blood cell in platelet concentrates met the standard (<0.2x1010/unit). The mean±S.D.: PRP-PC and BC-PC (day 1) were 0.030±0.017 x1010and 0.026±0.019x1010/unit (p-value=0.040), while PRP-PC and BC-PC (day 5) were0.033±0.013x1010 and 0.027±0.019x1010/unit (p-value= 0.580). (4) The pH of all units (PRP-PC and BC-PC) met the standard (≥6.2). The mean±S.D.: PRP-PC and BC-PC (day 1) were 7.430±0.330 and 7.750±0.160 (p-value=0.006), while PRP-PC and BC-PC (day 5) were 7.590±0.350 and 7.620±0.280 (p-value=0.710). The swirling phenomenon score and hypotonic shock response were the same as standard AABB and were not statistically difference.Conclusion: The quality of PRP-PC and BC-PC after storing on days 1 and 5 as follow (1) Volume of PRP-PC and BC-PC met the standard. (2) The platelet count per unit of PRP-PC and BC-PC (day 1), PRP-PC and BC-PC (day 5) were not statistically difference. (3) The white blood cell count per unit of PRP-PC and BC-PC (day 1) were statistically difference, while PRP-PC and BC-PC (day 5) were not statistically difference. (4) The pH of PRP-PC and BC-PC (day 1) were statistically difference, while PRP-PC and BC-PC (day 5) were not statistically difference. The swirling phenomenon score and hypotonic shock response of PRP-PC and BC-PC were not statistically difference. Platelet concentrates of both method storing on days 1 and 5 fulfilled the quality guideline of AABB.

White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods

Vox Sanguinis ◽  
2005 ◽  
Vol 88 (4) ◽  
pp. 275-277 ◽  
Author(s):  
M. J. Dijkstra-Tiekstra ◽  
C. E. van der Schoot ◽  
R. N. I. Pietersz ◽  
H. W. Reesink
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Platelet Rich Plasma ◽  
Buffy Coat ◽  
Platelet Concentrates ◽  
Cell Fragments

Blood Cell Count and the Presence or Absence of Infection in Venous Ulcers Treated With Platelet-Rich Plasma

2021 ◽  
Vol 33 (5) ◽  
pp. 113-118
Author(s):  
Beatriz de Oliveira ◽  
Joyce de Abreu Castro ◽  
Bruna Pires ◽  
Márcia de Assunção Ferreira ◽  
Jane Pinto ◽  
...  
Keyword(s):  
Blood Cell ◽  
Wound Infection ◽  
Cell Count ◽  
White Blood Cell ◽  
Platelet Rich Plasma ◽  
P Value ◽  
Blood Cell Count ◽  
Venous Ulcers ◽  
Cell Counts ◽  
Case Series Study

Introduction. In general, chronic wounds are colonized by bacteria; however, when microorganisms start to multiply at higher levels, wounds can become infected, causing prolongation of the inflammatory phase and retardation of collagen synthesis and epithelialization. Objective. The objective of this study was to evaluate the presence of infection in venous ulcers after 12 weeks of treatment with autologous platelet-rich plasma (PRP) and determine global white blood cell counts. Materials and Methods. This case series study involved a sequential sample of 17 patients with venous ulcers treated with PRP for 12 weeks. Descriptive and inferential statistical analysis was performed using the McNemar test and χ² test. Results. At baseline, 10 patients (58.8%) had wound infection. During the sixth week of treatment with PRP, only 3 patients (17.6%) continued to exhibit wound infection. After 12 weeks of PRP treatment, only 1 patient (5.9%) continued to exhibit wound infection. McNemar and χ² tests used to assess the presence of infection in the intervention group produced a P value of .0039 for a comparison of baseline and week 6 and a P value of .0078 for a comparison of baseline and week 12. These results demonstrated significant differences from baseline at both 6 weeks and 12 weeks of treatment, with greater significance at 12 weeks. There was no relationship between global white blood cell count and the presence of infection. Conclusion. After intervention with PRP, 94% of patients experienced improvement concerning the infection of ulcers.

scholarly journals Qualitative Evaluation of Platelet Concentrates Prepared with Different Methods

2021 ◽  
Author(s):  
. Varshashree ◽  
Subhashis Das ◽  
K Prabhakar
Keyword(s):  
Blood Cell ◽  
Tertiary Care ◽  
Quality Parameters ◽  
Platelet Concentrate ◽  
Director General ◽  
P Value ◽  
Platelet Concentrates ◽  
Distribution Width ◽  
Platelet Indices ◽  
Arterial Blood

Introduction: One of the most important responsibility of Blood Bank is to ensure uninterrupted supply of quality blood products, namely Platelet Concentrate (PC), which are in high demand particularly during pandemic. In India, PCs are of three types prepared by different methods like Platelet Rich Plasma-Platelet Concentrate (PRP-PC), Buffy Coat Platelet Concentrates (BC- PC), and Single Donor Platelets (SDP) units. Hence, establishing optimum quality standards across all the three types of PC remains a challenge in order to maximum benefit to recipients. Aim: To assess and compare the quality parameters of platelet concentrate prepared by PRP-PC, BC-PC and apheresis methods (SDP). Materials and Methods: This laboratory based observational study was conducted at rural, tertiary care, postgraduate, teaching hospital located at South India from December 2018 to November 2019. A total of 156 PCs were studied with 52 units belonging to PRP method, 52 units BC-PC method and the remaining were 52 units of SDP prepared by apheresis method. Quality parameters include volume, pH, swirling, White Blood Cell (WBC) count/bag, platelet count/bag and platelet indices. Platelet indices include Mean Platelet Volume (MPV), Platelet Distribution Width (PDW), and Platelet Large Cell Ratio (PLCR). Arterial Blood Gas (ABG) analysis was also done to assess (pH, partial pressure of Carbon Dioxide (pCO2), plasma bicarbonate (pHCO3-)). The p-value less than 0.05 were considered statistically significant. Statistical Package for the Social Sciences (SPSS) software version 23.0 used for statistical analysis. Results: All the 156 PC units were analysed as per the Director General of Health Services (DGHS) India, criteria. Among the 156 platelet concentrates scored as per DGHS criteria, a score of 5 was obtained for 34.61% [18/52], 30.76 [16/52] and 11.5% [6/52] SDP, BC-PC and PRP-PC units, respectively. All the PC units were sterile and Red Blood Cell (RBC) contamination was within acceptable limits. Conclusion: In this study, quality parameters of SDP was better than BC-PC and PRP-PC but with improvement and more standardisation of procedures and storage, BC-PC units can give a yield similar to that of SDP units.

Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction

Vox Sanguinis ◽  
2004 ◽  
Vol 87 (4) ◽  
pp. 250-256 ◽  
Author(s):  
M. J. Dijkstra-Tiekstra ◽  
C. E. van der Schoot ◽  
R. N. I. Pietersz ◽  
P. C. Huijgens ◽  
P. F. van der Meer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Cell ◽  
Real Time ◽  
White Blood Cell ◽  
Platelet Concentrates ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
And Storage ◽  
Cell Fragments

scholarly journals Decrease in white blood cell counts after thiopentone barbiturate therapy for refractory intracranial hypertension: A common complication

2013 ◽  
Vol 04 (S 01) ◽  
pp. S31-S34 ◽  
Author(s):  
Shin Yi Ng ◽  
Ki Jinn Chin ◽  
Tong Kiat Kwek
Keyword(s):  
Statistical Analysis ◽  
Blood Cell ◽  
Intracranial Hypertension ◽  
White Blood Cell ◽  
Retrospective Review ◽  
T Test ◽  
Barbiturate Coma ◽  
Barbiturate Therapy ◽  
The Mean ◽  
Wbc Count

ABSTRACT Background: Leucopenia has been reported after induction of thiopentone barbiturate therapy for refractory intracranial hypertension. However, the incidence and characterisitics are not well described. Aims: We performed a retrospective review to describe the incidence and characteristics of leucopenia after induction of thiopentone barbiturate therapy. Setting and Design: Our centre is a national referral centre for neurotrauma and surgery in a tertiary medical institution.Materials and Methods: We performed a retrospective review of all patients who received thiopentone barbiturate therapy for refractory intracranial hypertension during an 18 month period from January 2004 to June 2005 in our neurosurgical intensive care unit. Statistical Analysis Used: Statistical analysis was performed using SPSS version 15.0. All data are reported as mean ± standard deviation or median (interquartile range). The Chi square test was used to analyze categorical data and student t test done for comparison of means. For paired data, the paired t?test was used.-test was used. Results: Thirty eight (80.9%) out of 47 patients developed a decrease in white blood cell (WBC) count after induction of thiopentone barbiturate coma. The mean decrease in WBC from baseline to the nadir was 6.4 × 10 9 /L (P <lt; 0.001) and occurred 57 (3-147) h after induction. The mean nadir WBC was 8.6 < 3.6 × 10 9 /L. Three (6.4%) patients were leucopenic, with a WBC count of 2.8, 3.1, and 3.6 < 10 9 /L. None of them were neutropenic. We did not find an association between decrease in WBC count and clinical diagnosis of infection. We did not find any association between possible risk factors such as admission GCS, maximum ICP prior to induction of barbiturate coma, APACHE II score, total duration and dose of thiopentone given, and decrease in WBC count. Conclusions: Decrease in WBC count is common, while development of leucopenia is rare after thiopentone barbiturate coma. Regular monitoring of WBC counts is recommended.

Differences in Residual White Blood Cell Subset Counts in Buffy Coat-Depleted Red Cell Concentrates Prepared with Bottom and Top or Quadruple-Bag Systems

Vox Sanguinis ◽  
1999 ◽  
Vol 77 (2) ◽  
pp. 97-102 ◽  
Author(s):  
J. Wildt-Eggen ◽  
J.G. Schrijver ◽  
P.A. Kuiper-Kramer ◽  
M. Bins ◽  
H.C. Prooijen
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Cell Subset ◽  
Buffy Coat ◽  
Red Cell
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