scholarly journals External quality assessment of AFB smear microscopy performances and its associated factors in selected private health facilities in Addis Ababa, Ethiopia

Author(s):  
Lemi Mosissa ◽  
Abebaw Kebede ◽  
Tedla Mindaye ◽  
Muluwork Getahun ◽  
Sisay Tulu ◽  
...  

Background: In several countries with a high incidence of tuberculosis (TB), direct sputum smear microscopy remains the most cost effective tool for diagnosing patients with infectious tuberculosis and for monitoring their progress on treatment. The objective of the current study was to assess the quality of laboratory service in AFB microscopy of Tigray Region. Randomly selected health facilities were participated in the external quality assessment. Data was entered and analysed using SPSS version 20. Results: Between December 2015 and February 2016, a total of 80 laboratories were participated. Most (87%) of the laboratories have continuous electric supply. Only 70% of the health facilities have continuous pipe water and 56.5 % have no separate table for smear preparation, staining, microscopy and recording. From the distributed panel slides, 91% of the Health facilities were scored 100% concordant results but 9% of the health facilities were scored discordant results. The main reasons for discordant results were shortage of training, non-functional microscope, have no lens cleaning and using of unfiltered carbol fuchsin reagent. Conclusions: The performance of health facilities for smear microscopy reading and smear quality were relatively low in this study area, because smear positivity rate and annual negative slide volume of the health facilities were below the standard. Therefore Strengthening the EQA program and technical support on smear quality indicators are recommended to ensure for better quality diagnostic service of tuberculosis


2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Banchamlak Tegegne ◽  
Kefale Ejigu ◽  
Getaneh Alemu ◽  
Yeshimebet Fetene ◽  
Kindye Endaylalu ◽  
...  

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Habtamu Asrat ◽  
Abebaw Kebede ◽  
Abnet Abebe ◽  
Abyot Meaza ◽  
Getinet Hailu ◽  
...  

Background: Tuberculosis is an infectious disease caused by the bacillus Mycobacterium tuberculosis. According to the Ethiopian Federal Ministry of Health’s 2013–2014 report, the tuberculosis case detection rate was 53.7%, which was below the target of 81% set for that year.Objective: This study assessed the performance of tuberculosis smear microscopists at external quality assessment rechecking laboratories in Ethiopia.Methods: A cross-sectional study was conducted at 81 laboratories from April to July 2015. Panel slides were prepared and validated at the National Tuberculosis Reference Laboratory. The validated panel slides were used to evaluate the performance of microscopists at these laboratories compared with readers from the reference laboratory.Results: A total of 389 external quality assessment rechecking laboratory microscopists participated in the study, of which 268 (68.9%) worked at hospitals, 241 (62%) had more than five years of work experience, 201 (51.7%) held Bachelors degrees, and 319 (82%) reported tuberculosis smear microscopy training. Overall, 324 (83.3%) participants scored ≥ 80%. Sensitivity for detecting tuberculosis bacilli was 84.5% and specificity was 93.1%. The overall percent agreement between participants and reference readers was 87.1 (kappa=0.72). All 10 slides were correctly read (i.e., scored 100%) by 80 (20.6%) participants, 156 (40.1%) scored 90% – 95%, 88 (22.6%) scored 80% – 85% and 65 (16.7%) scored below 80%. There were 806 (20.7%) total errors, with 143 (3.7%) major and 663 (17%) minor errors.Conclusion: The overall performance of participants in reading the slides showed good agreement with the reference readers. Most errors were minor, and the ability to detect tuberculosis bacilli can be improved through building the capacity of professionals.


2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Desalegn Admassu Ayana ◽  
Zelalem Teklemariam Kidanemariam ◽  
Habtamu Mitiku Tesfaye ◽  
Fitsum Weldegebreal Milashu

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Hiwot Amare Hailu ◽  
Melashu Balew Shiferaw ◽  
Leykun Demeke ◽  
Mulatu Melese Derebe ◽  
Zelalem Dessie Gelaw ◽  
...  

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Eva Chang ◽  
Fabio Luelmo ◽  
Zamira Baydulloeva ◽  
Marija Joncevska ◽  
Guljamol Kasymova ◽  
...  

Introduction: Tajikistan has a laboratory network with three levels of tuberculosis (TB) laboratories. The external quality assessment (EQA) of sputum smear microscopy was implemented in 2007. The objective of this study was to evaluate the EQA system and identify potential performance improvement strategies in TB microscopic laboratories in Sughd, Tajikistan.Methods: This is a cross-sectional study based on retrospective record review and secondary data analyses on Acid-Fast Bacilli (AFB) microscopy data and EQA reading results collected between the first quarter of 2011 and the fourth quarter of 2013. Descriptive analyses were conducted to examine the overview of microscopy laboratories activities, EQA results, and laboratory performance. Result: Of the 123,874 smears examined between 2011 and 2013, 11,522 (9.30%) were re-checked by the EQA system. The population TB screening rate rose from 0.46% in 2011 to 0.57% in 2013, and the case positivity rate decreased from 6.98% to 4.80%. The regional EQA results showed a reduction in high false-positive, high false-negative, and low false-negative errors. False-positive errors had decreased from 0.13% in 2011 to 0.07% in 2013, and false-negative errors from 0.91% in 2011 to 0.15% in 2013. Regional sensitivity of smear microscopy, when compared to re-checking controller, increased from 88.2% in 2011 to 97.2% in 2013. The regional specificity level remained relatively stable at above 99%.Conclusion: Our study found that a decreasing trend of case positivity rate from 2011 to 2013 in Sughd, though the overall laboratory workload was on the rise. In addition, EQA results showed an overall error reduction and an improved sensitivity of smear microscopy in the region. The overview of microscopic laboratory activities and the actual evaluation of the EQA system on sputum smear microscopy complement each other in providing a better picture on the progress of TB laboratory strengthening. We recommend similar approaches to be adapted by future evaluations on TB microscopic laboratories, particularly among countries of high burden. Interactive training and feedback loops are crucial to improving TB surveillance in Tajikistan.


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