Diarrheal diseases and household sanitary practices in Kakuuto County, Rakai District, Uganda, 2016

Author(s):  
Richardson Mafigiri ◽  
Alex Riolexus Ario ◽  
Federick Makumbi
2014 ◽  
Vol 306 (2) ◽  
pp. G123-G131 ◽  
Author(s):  
Arivarasu N. Anbazhagan ◽  
Shubha Priyamvada ◽  
Anoop Kumar ◽  
Daniel B. Maher ◽  
Alip Borthakur ◽  
...  

SLC26A3 [downregulated in adenoma (DRA)] is a Cl−/HCO3− exchanger involved in electroneutral NaCl absorption in the mammalian intestine. Altered DRA expression levels are associated with infectious and inflammatory diarrheal diseases. Therefore, it is critical to understand the regulation of DRA expression. MicroRNAs (miRNAs) are endogenous, small RNAs that regulate protein expression via blocking the translation and/or promoting mRNA degradation. To investigate potential modulation of DRA expression by miRNA, five different in silico algorithms were used to predict the miRNAs that target DRA. Of these miRNAs, miR-494 was shown to have a highly conserved putative binding site in the DRA 3′-untranslated region (3′-UTR) compared with other DRA-targeting miRNAs in vertebrates. Transfection with pmirGLO dual luciferase vector containing DRA 3′-UTR (pmirGLO-3′-UTR DRA) resulted in a significant decrease in relative luciferase activity compared with empty vector. Cotransfection of the DRA 3′-UTR luciferase vector with a miR-494 mimic further decreased luciferase activity compared with cells transfected with negative control. The transfection of a miR-494 mimic into Caco-2 and T-84 cells significantly increased the expression of miR-494 and concomitantly decreased the DRA protein expression. Mutation of the seed sequences for miR-494 in 3′-UTR of DRA abrogated the effect of miR-494 on 3′-UTR. These data demonstrate a novel regulatory mechanism of DRA expression via miR-494 and indicate that targeting this microRNA may serve to be a potential therapeutic strategy for diarrheal diseases.


1996 ◽  
Vol 1 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Quazi Manjurul Haque ◽  
Hitoshi Yoshimura ◽  
Yutaka Midorikawa ◽  
Satoshi Nakamura ◽  
Akira Sugiyama ◽  
...  

2002 ◽  
Vol 33 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Richard L. Guerrant ◽  
Margaret Kosek ◽  
Sean Moore ◽  
Breyette Lorntz ◽  
Richard Brantley ◽  
...  
Keyword(s):  

Gut ◽  
1992 ◽  
Vol 33 (6) ◽  
pp. 863-863 ◽  
Author(s):  
M J G Farthing
Keyword(s):  

1977 ◽  
Vol 62 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Eugene J. Gangarosa

1975 ◽  
Vol 43 (3) ◽  
pp. 341-346 ◽  
Author(s):  
Reynaldo Martorell ◽  
Charles Yarbrough ◽  
Aaron Lechtig ◽  
Jean-Pierre Habicht ◽  
Robert E. Klein

2014 ◽  
Vol 32 (1) ◽  
pp. 26-30 ◽  
Author(s):  
MUH Begum

The World Health Organization (WHO) and the American Academy of Pediatrics (AAP) emphasize the value of breastfeeding for mothers as well as children. Both recommend exclusive breastfeeding for the first six months of life. Human breast milk is the healthiest form of milk for babies. Breastfeeding promotes health and helps to prevent diseases including diarrheal diseases. It contains all nutrients including antibodies (IgA),and lactoferrin, that potentially prevent infection and diarrhea in infants and children. Studies conducted in both developed and under developed nations have found that breast feeding is associated with significantly ( upto 64%) less diarrheal disease and the protective effect of breast feeding does not persist beyond two months after breast feeding is stopped. On the other hand, formula fed infants are found an upto 80% increased in the risk of developing diarrhea compared to breast fed infants and there is significantly more diarrheal disease in formula fed infants. Infection may be attributable to contamination of bottles, teats, milk, and food in infants who are not exclusively breastfed. Exclusive breastfeeding for the first six months of life and there after complementary feedings while breastfeeding continues for up to two years of age or beyond, enthusiastic support and involvement from clinicians, obstetricians and pediatricians, are essential in “breastfeeding vs formula feeding” issue and to reduce incidence of diarrheal diseases in infants and children. DOI: http://dx.doi.org/10.3329/jbcps.v32i1.21033 J Bangladesh Coll Phys Surg 2014; 32: 26-30


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