Repair of Critical-Size Bone Defects Using Bone Marrow Stem Cells or Autogenous Bone With or Without Collagen Membrane: A Histomorphometric Study in Rabbit Calvaria

2015 ◽  
Vol 30 (1) ◽  
pp. 208-215 ◽  
Author(s):  
Antonio Aloise ◽  
André Pelegrine ◽  
Allan Zimmermann ◽  
Rafael de Mello e Oliveira ◽  
Lydia Ferreira
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Critical Size ◽  
Bone Defects ◽  
Bone Marrow Stem Cells ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Histomorphometric Study

scholarly journals Slowly Delivered Icariin/Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells to Promote the Healing of Calvarial Critical-Size Bone Defects

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Tianlin Liu ◽  
Xin Zhang ◽  
Yuan Luo ◽  
Yuanliang Huang ◽  
Gang Wu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Large Volume ◽  
Critical Size ◽  
Bone Defects ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Trabecular Thickness

Bone tissue engineering technique is a promising strategy to repair large-volume bone defects. In this study, we developed a 3-dimensional construct by combining icariin (a small-molecule Chinese medicine), allogeneic bone marrow-derived mesenchymal stem cells (BMSCs), and a siliceous mesostructured cellular foams-poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (SMC-PHBHHx) composite scaffold. We hypothesized that the slowly released icariin could significantly promote the efficacy of SMC-PHBHHx/allogeneic BMSCs for repairing critical-size bone defects in rats. In in vitro cellular experiments, icariin at optimal concentration (10−6 mol/L) could significantly upregulate the osteogenesis- and angiogenesis-related genes and proteins, such as Runx2, ALP, osteocalcin, vascular endothelial growth factors, and fibroblast growth factors, as well as the mineralization of BMSCs. Icariin that was adsorbed onto the SMC-PHBHHx scaffold showed a slow release profile within a 2-week monitoring span. Eight weeks after implantation in calvarial critical-size bone defects, the constructs with icariin were associated with significantly higher bone volume density, trabecular thickness, trabecular number, and significantly lower trabecular separation than the constructs without icariin. Histomorphometric analysis showed that icariin was also associated with a significantly higher density of newly formed blood vessels. These data suggested a promising application potential of the icariin/SMC-PHBHHx/allogeneic BMSCs constructs for repairing large-volume bone defects in clinic.

scholarly journals Cell Scaffolds for Bone Tissue Engineering

2020 ◽  
Vol 7 (4) ◽  
pp. 119
Author(s):  
Kazutoshi Iijima ◽  
Hidenori Otsuka
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defects ◽  
Autogenous Bone ◽  
Cell Scaffolds

Currently, well-known surgical procedures for bone defects are classified into four types: (1) autogenous bone graft transplantation, (2) allogeneic bone graft transplantation, (3) xenogeneic bone graft transplantation, and (4) artificial bone graft transplantation. However, they are often risky procedures and related to postoperative complications. As an alternative, tissue engineering to regenerate new bone often involves the use of mesenchymal stem cells (MSCs), derived from bone marrow, adipose tissues, and so on, which are cultured into three-dimensional (3D) scaffolds to regenerate bone tissue by osteoinductive signaling. In this manuscript, we provide an overview of recent treatment of bone defects and the studies on the creation of cell scaffolds for bone regeneration. Bone regeneration from bone marrow-derived mesenchymal stem cells using silica nonwoven fabric by the authors’ group were provided. Potential application and future direction of the present systems were also described.

scholarly journals Alveolar Cleft Reconstruction Using Different Grafting Techniques

2019 ◽  
Vol 7 (8) ◽  
pp. 1369-1373 ◽  
Author(s):  
Aida Mossaad ◽  
Tarek El Badry ◽  
Moustapha Abdelrahman ◽  
Ahmad Abd Elazeem ◽  
Wael Ghanem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Iliac Crest ◽  
Cleft Lip And Palate ◽  
Calcium Hydroxyapatite ◽  
Bone Marrow Stem Cells ◽  
Collagen Membrane ◽  
Alveolar Cleft ◽  
Group A ◽  
Group B

BACKGROUND: Cleft lip and palate CLP is a frequent congenital malformation that manifests in several varieties including unilateral or bilateral anomalies due to either genetic or acquired causes. Alveolar cleft graft ACG remains controversial as regard timing, grafting materials and surgical techniques. The primary goal of alveolar cleft grafting in ACG patients is to provide an intact bony ridge at the cleft site to allow maxillary continuity for teeth eruption, proper orthodontic treatment for dental arch alignment, oronasal fistula closure and providing alar support for nasal symmetry. AIM: This study aims to compare different grafting techniques to treat the alveolar cleft defect. METHODS: This study included 24 cases divided into three groups of patients: Group A was treated with autogenous iliac crest bone; Group B was treated with nano calcium hydroxyapatite with collagen membrane and Group C was treated with tissue engineering method using bone marrow stem cells extract and PRF membrane. RESULTS: According to clinical and radiographic examination measuring bone density in the CT preoperatively compared to six months postoperatively. Group C with bone marrow stem cells extract showed superior results among all followed by group B, while group A with autogenous iliac crest showed resorption in some cases and gave the least values, in addition to its drawbacks as regard donor site affection with pain & scar formation. CONCLUSION: Bone substitutes as Nano calcium hydroxyapatite and bone marrow stem cells extract showed to be reliable methods for bone grafting than autogenous iliac crest.

Bone marrow stem cells make hepatocytes with replicative potential

2001 ◽  
Vol 120 (5) ◽  
pp. A62-A62
Author(s):  
S FORBES ◽  
M ALISON ◽  
K HODIVALADILKE ◽  
R JEFFERY ◽  
R POULSOM ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells
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