scholarly journals Tissue-specific expression of type 1 angiotensin II receptor subtypes. An in situ hybridization study.

Hypertension ◽  
1994 ◽  
Vol 24 (5) ◽  
pp. 531-537 ◽  
Author(s):  
J M Gasc ◽  
S Shanmugam ◽  
M Sibony ◽  
P Corvol
Keyword(s):  
In Situ Hybridization ◽  
Angiotensin Ii ◽  
Receptor Subtypes ◽  
Hybridization Study ◽  
Angiotensin Ii Receptor ◽  
Specific Expression ◽  
Tissue Specific Expression ◽  
Angiotensin Ii Receptor Subtypes

Ontogeny of the two angiotensin II type 1 receptor subtypes in rats

1994 ◽  
Vol 267 (6) ◽  
pp. E828-E836 ◽  
Author(s):  
S. Shanmugam ◽  
P. Corvol ◽  
J. M. Gasc
Keyword(s):  
In Situ Hybridization ◽  
Angiotensin Ii ◽  
Adrenal Gland ◽  
Cerebral Arteries ◽  
Receptor Subtypes ◽  
Angiotensin Ii Receptor ◽  
Receptor Mrna ◽  
Young Rat

The two subtypes (AT1A and AT1B) of the type 1 (AT1) angiotensin II receptor mRNA were localized by in situ hybridization in rat fetal tissues from day 11 to 19 of gestation and in the young rat from day 0 to 10 postpartum, by use of 35S-labeled cRNA probes. Both subtype mRNAs were present in the kidney and in the adrenal gland. Organs such as liver, lung, heart, and undifferentiated mesenchymes expressed only AT1A mRNA. In contrast to the adult, only AT1A subtype was expressed during fetal and postnatal periods in the pituitary gland. Large blood vessels (e.g., aorta and cerebral arteries) expressed exclusively AT1A mRNA during fetal stages. The expression of each subtype appears to be differentially regulated, in a tissue- and age-specific way. This spatotemporal regulation of AT1A and AT1B expression suggests that angiotensin II could act as a differentiation factor during organogenesis in addition to its classical role as a regulator of the cardiovascular system.

Tissue-Specific Expression of Human Angiotensin II AT1 and AT2 Receptors and Cellular Localization of Subtype mRNAs in Adult Human Renal Cortex Using in situ Hybridization

Nephron ◽  
1998 ◽  
Vol 80 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Hiroaki Matsubara ◽  
Takeshi Sugaya ◽  
Satoshi Murasawa ◽  
Yoshihisa Nozawa ◽  
Yasukiyo Mori ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Angiotensin Ii ◽  
Cellular Localization ◽  
Renal Cortex ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Adult Human ◽  
At2 Receptors

Distribution of angiotensin II receptor subtypes in rat and human kidney

1992 ◽  
Vol 262 (2) ◽  
pp. F236-F240 ◽  
Author(s):  
L. A. Sechi ◽  
E. F. Grady ◽  
C. A. Griffin ◽  
J. E. Kalinyak ◽  
M. Schambelan
Keyword(s):  
Angiotensin Ii ◽  
Binding Affinity ◽  
Human Kidney ◽  
Angiotensin Ii Receptors ◽  
Receptor Subtypes ◽  
Renal Tubules ◽  
Physiological Effects ◽  
Angiotensin Ii Receptor ◽  
Angiotensin Ii Receptor Subtypes

Angiotensin II initiates a variety of physiological effects in the kidney by binding to high-affinity receptors on plasma membranes. Recently, two subtypes of angiotensin II receptors have been distinguished on the basis of differences in signal transduction mechanisms, binding affinity to agonists and antagonists, and inhibition of binding by dithiothreitol. To evaluate the density and distribution of these receptor subtypes in the kidney, we performed an in situ autoradiographic study on frozen tissue sections obtained from rat and human kidneys. Sections were incubated with 125I-[Sar1,Ile8]angiotensin II and binding specificity was verified by competition with unlabeled [Sar1]angiotensin II. Angiotensin II receptor subtypes were characterized by competition with the nonpeptide receptor antagonists, DuP 753 (type 1) and PD123177 (type 2). Both rat and human kidney exhibited a high concentration of angiotensin II receptors in glomeruli and in the longitudinal bands traversing the outer portion of the medulla, corresponding to the medullary vascular bundles. Binding affinity (Kd = 0.6 +/- 0.4 nM), determined in rat kidney, was similar to that reported previously in isolated glomeruli and membrane vesicles prepared from renal tubules. Angiotensin II binding was almost completely inhibited by DuP 753, whereas PD123177 had little effect. Thus the predominant angiotensin II receptor subtype in both rat and human kidney is type 1. The distribution of angiotensin II receptors correlates well with the intrarenal sites at which the peptide has its major physiological effects.

Type 1 Angiotensin II Receptor Subtypes in Kidney of Normal and Salt-Sensitive Hypertensive Rats

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 392-398 ◽  
Author(s):  
Nadine Bouby ◽  
Lise Bankir ◽  
Catherine Llorens-Cortes
Keyword(s):  
Angiotensin Ii ◽  
Receptor Subtypes ◽  
Angiotensin Ii Receptor ◽  
Hypertensive Rats ◽  
Angiotensin Ii Receptor Subtypes

scholarly journals Desensitization of Type 1 Angiotensin II Receptor Subtypes in the Rat Kidney

Endocrinology ◽  
2001 ◽  
Vol 142 (11) ◽  
pp. 4683-4692 ◽  
Author(s):  
A. Hus-Citharel ◽  
N. Bouby ◽  
J. Marchetti ◽  
D. Chansel ◽  
D. Goidin ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Rat Kidney ◽  
Receptor Subtypes ◽  
Angiotensin Ii Receptor ◽  
Angiotensin Ii Receptor Subtypes

Analysis of Angiotensin II Receptor Subtypes in Individual Rat Brain Nuclei

1992 ◽  
Vol 55 (5) ◽  
pp. 563-573 ◽  
Author(s):  
Brian P. Rowe ◽  
David L. Saylor ◽  
Robert C. Speth
Keyword(s):  
Angiotensin Ii ◽  
Rat Brain ◽  
Receptor Subtypes ◽  
Angiotensin Ii Receptor ◽  
Brain Nuclei ◽  
Angiotensin Ii Receptor Subtypes
Sign in / Sign up

Export Citation Format

Share Document