Skeletal Muscle–Specific Myosin Binding Protein-H Is Expressed in Purkinje Fibers of the Cardiac Conduction System

1997 ◽  
Vol 80 (5) ◽  
pp. 665-672 ◽  
Author(s):  
Tatiana Alyonycheva ◽  
Leona Cohen-Gould ◽  
Christiana Siewert ◽  
Donald A. Fischman ◽  
Takashi Mikawa
Keyword(s):  
Skeletal Muscle ◽  
Binding Protein ◽  
Conduction System ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Purkinje Fibers ◽  
Myosin Binding Protein ◽  
Myosin Binding

Slow skeletal muscle myosin-binding protein-C (MyBPC1) mediates recruitment of muscle-type creatine kinase (CK) to myosin

2011 ◽  
Vol 436 (2) ◽  
pp. 437-445 ◽  
Author(s):  
Zhe Chen ◽  
Tong-Jin Zhao ◽  
Jie Li ◽  
Yan-Song Gao ◽  
Fan-Guo Meng ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Creatine Kinase ◽  
Muscle Contraction ◽  
Binding Protein ◽  
High Energy ◽  
Functional Coupling ◽  
Muscle Type ◽  
Myosin Binding Protein ◽  
Slow Skeletal Muscle ◽  
Myosin Binding

Muscle contraction requires high energy fluxes, which are supplied by MM-CK (muscle-type creatine kinase) which couples to the myofibril. However, little is known about the detailed molecular mechanisms of how MM-CK participates in and is regulated during muscle contraction. In the present study, MM-CK is found to physically interact with the slow skeletal muscle-type MyBPC1 (myosin-binding protein C1). The interaction between MyBPC1 and MM-CK depended on the creatine concentration in a dose-dependent manner, but not on ATP, ADP or phosphocreatine. The MyBPC1–CK interaction favoured acidic conditions, and the two molecules dissociated at above pH 7.5. Domain-mapping experiments indicated that MM-CK binds to the C-terminal domains of MyBPC1, which is also the binding site of myosin. The functional coupling of myosin, MyBPC1 and MM-CK is further corroborated using an ATPase activity assay in which ATP expenditure accelerates upon the association of the three proteins, and the apparent Km value of myosin is therefore reduced. The results of the present study suggest that MyBPC1 acts as an adaptor to connect the ATP consumer (myosin) and the regenerator (MM-CK) for efficient energy metabolism and homoeostasis.

scholarly journals Why Do We Have Purkinje Fibers Deep in Our Heart?

2014 ◽  
pp. S9-S18 ◽  
Author(s):  
D. SEDMERA ◽  
R. G. GOURDIE
Keyword(s):  
Conduction System ◽  
Human Anatomy ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Model Species ◽  
Purkinje Fibers ◽  
Current State

Purkinje fibers were the first discovered component of the cardiac conduction system. Originally described in sheep in 1839 as pale subendocardial cells, they were found to be present, although with different morphology, in all mammalian and avian hearts. Here we review differences in their appearance and extent in different species, summarize the current state of knowledge of their function, and provide an update on markers for these cells. Special emphasis is given to popular model species and human anatomy.

Abstract 331: Loss of Myosin Binding Protein H-Like Causes Cardiac Conduction Abnormalities

2019 ◽  
Vol 125 (Suppl_1) ◽  
Author(s):  
David Y Barefield ◽  
Sean Yamakawa ◽  
Ibrahim Tahtah ◽  
Jordan J Sell ◽  
Michael Broman ◽  
...  
Keyword(s):  
Binding Protein ◽  
Cardiac Conduction ◽  
Myosin Binding Protein ◽  
Conduction Abnormalities ◽  
Myosin Binding

scholarly journals Increased expression of Myosin binding protein H in the skeletal muscle of amyotrophic lateral sclerosis patients

2014 ◽  
Vol 1842 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Antonio Conti ◽  
Nilo Riva ◽  
Mariasabina Pesca ◽  
Sandro Iannaccone ◽  
Carlo V. Cannistraci ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amyotrophic Lateral Sclerosis ◽  
Binding Protein ◽  
Myosin Binding Protein ◽  
Myosin Binding ◽  
Lateral Sclerosis

Dynamical behavior of cardiac conduction system under external disturbances: simulation based on microcontroller technology

2022 ◽  
Author(s):  
Rodrigue Fonkou ◽  
Patrick Louodop ◽  
Pierre Kisito Talla
Keyword(s):  
Blood Pressure ◽  
Electric Current ◽  
Sinus Node ◽  
Dynamical Behavior ◽  
Heart Rhythm ◽  
Conduction System ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Very High Frequency ◽  
Purkinje Fibers

Abstract The heart rhythm is one of the most interesting aspects of the dynamic behavior of biological systems. Understanding heart rhythms is essential in the dynamic analysis of the heart. Each type of dynamic behaviour can describe normal or pathological physiology. The heart is made up of nodes ranging from SA node (natural pacemaker) to Purkinje fibers. The electric current originates in the sinus node and travels through the heart until it reaches the Purkinje fibers, causing after its passage through each of the nodes a heartbeat thus constituting the electrocardiogram (ECG). Since the origin of the electric current is the sinus node, in this article we study numerically and experimentally by microcontroller the influence of the sinus node on the propagation of electric current through the heart. A study of the sinus node in its autonomous state shows us that in their coupled state, the nodes of the heart qualitatively reproduce the time series of the action potential of this latter, which leads to the recording of the ECG. A study when the sinus node is subjected to periodic pulsed excitation E 1(t) = kP(t), assumed to come from blood pressure, with P(t) the blood pressure, shows that for some selected frequencies, it is found that the nodes of the heart and the ECG exhibit responses having the same shape and the same frequencies as those of the pulsatile blood pressure. This suggests the possibility of using such a conversion and excitation mechanism to replicate the functioning of cardiac conduction system. The chaotic analysis of the sinus node subjected to a sinusoidal type disturbance (E 0sin(ωt)) is also presented, it shows that in its chaotic state, the nodes of the heart, as well as the ECG, provide very high frequency signals. This requires the control of the sinus node (natural pacemaker) in such a situation

scholarly journals Distribution and Structure of Purkinje Fibers in the Heart of Ostrich (Struthio camelus) with the Special References on the Ultrastructure

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Paria Parto ◽  
Mina Tadjalli ◽  
S. Reza Ghazi ◽  
Mohammad Ali Salamat
Keyword(s):  
Light And Electron Microscopy ◽  
Great Part ◽  
Conduction System ◽  
Point Of View ◽  
Cardiac Conduction ◽  
Cardiac Conduction System ◽  
Purkinje Fibers ◽  
Thin Ring ◽  
T Tubules ◽  
P Cells

Purkinje fibers or Purkinje cardiomyocytes are part of the whole complex of the cardiac conduction system, which is today classified as specific heart muscle tissue responsible for the generation of the heart impulses. From the point of view of their distribution, structure and ultrastructural composition of the cardiac conduction system in the ostrich heart were studied by light and electron microscopy. These cells were distributed in cardiac conducting system including SA node, AV node, His bundle and branches as well as endocardium, pericardium, myocardium around the coronary arteries, moderator bands, white fibrous sheet in right atrium, and left septal attachment of AV valve. The great part of the Purkinje fiber is composed of clear, structure less sarcoplasm, and the myofibrils tend to be confined to a thin ring around the periphery of the cells. They have one or more large nuclei centrally located within the fiber. Ultrastructurally, they are easily distinguished. The main distinction feature is the lack of electron density and having a light appearance, due to the absence of organized myofibrils. P-cells usually have two nuclei with a mass of short, delicate microfilaments scattered randomly in the cytoplasm; they contain short sarcomeres and myofibrillar insertion plaque. They do not have T-tubules.

Sign in / Sign up

Export Citation Format

Share Document